DGKA Antibodies

AND DGKA ELISA kits, DGKA Proteins

Many biological assays use antibodies to detect DGKA, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with DGKA in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop DGKA antibodies...

We validate the specificity of these antibodies to DGKA by testing them on tissues known to express DGKA positively and negatively. Browse below to find the DGKA antibody that suites your experiment. We have 5 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

Designing an IHC experiment for DGKA? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.

Open the DGKA IHC Guide

DGKA Infographic by Boster Bio

All DGKA Products

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Product

Figures

Specificity

Applications

Price

Anti-DGK- alpha Antibody

Immunofluorescence analysis of MCF7 cell

Western Blot analysis of various cells using DGK-α Polyclonal Antibody

Western Blot analysis of Lovo cells using DGK-α Polyclonal Antibody

Human, Mouse, Rat

ELISA, IF, IHC, WB

$399
Cat # A06354
100μl

Anti-DGKA Antibody Picoband®

Western blot analysis of DGKA using anti-DGKA antibody (A06354-3)

Flow Cytometry analysis of Jurkat cells using anti-DGKA antibody (A06354-3)

Human, Mouse, Rat

ELISA, Flow Cytometry, WB

$370
Cat # A06354-3
100 μg/vial

DGKA (NM_201444) Human Over-expression Lysate

DGKA Human Over-expression Lysates NM_001345

Human

$628
Cat # LS001606
20 µg, 100 µg

DGKA Colorimetric Cell-Based ELISA

Western blot analysis of extracts from Jurkat cells

Human

ELISA

$480
Cat # EKC1694
1 kit, containing one 96-well plate and all necessary reagents

DGKA (NM_201445) Human Recombinant Protein

Coomassie blue staining of purified DGKA protein

$1249
Cat # PROTP23743
20 µg

DGKA Overview

Facts about Diacylglycerol kinase alpha.

DGKA 3D structure. Source: alphafold

Diacylglycerol kinase alpha (DGKA)

Upon cell stimulation transforms the second messenger diacylglycerol into phosphatidate, initiating the resynthesis of phosphatidylinositols and attenuating protein kinase C action. .

Gene Information

Human
Gene Name:	DGKA
Uniprot:	P23743
Entrez:	1606

Family

Belongs to:

eukaryotic diacylglycerol kinase family

Alternative Names

DAG kinase alpha; DAGK; DAGK1; DAGK1diacylglycerol kinase, alpha (80kD); DGKA; DGKalpha; DGK-alpha; DGK-alphaEC 2.7.1.107; diacylglycerol kinase alpha; diacylglycerol kinase, alpha 80kDa; Diglyceride kinase alpha; MGC12821,80 kDa diacylglycerol kinase; MGC42356

Molecular Weight

Mass (kDA):
82.63 kDA

Genomic Context


Human

Location:	12q13.2
Sequence:	12; NC_000012.12 (55931091..55954023)

Tissue Specificity

Lymphocytes and oligodendroglial cells.

Diseases

Malignant Neoplasms
Adenocarcinoma
Cell Transformation, Neoplastic
Lung Neoplasms
Neoplasms
Pancreatic Neoplasm
Endometrial Polyp
Malignant Neoplasm Of Pancreas
Tissue Adhesions

Adenocarcinoma Of The Gastroesophageal Junction
Esophageal Neoplasms
Physiological Stress

Pathways

Cell Adhesion
Nodulation
Cell Cycle
Phospholipid Homeostasis
Sporulation
Insemination
Glycolysis

Embryo Development

PTMs

Phosphorylation

Research Areas

Lipid and Metabolism

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/DGKA

References

PMID: 2175712 by Schaap D., et al. Purification, cDNA-cloning and expression of human diacylglycerol kinase.

PMID: 8180475 by Hart T.C., et al. Assignment of the gene for diacylglycerol kinase (DAGK) to human chromosome 12.