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- Table of Contents
FOXJ1 · Western blot design guide
FOXJ1 Western Blot Planning Guide
Plan a FOXJ1 Western blot around the catalog-observed 45.2 kDa band, image-backed A04942-3 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
FOXJ1 Western Blot Experimental Design Guide
Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 45.2 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 12-15% | |
| Positive control | Fallopian tube | |
| Negative control | Adipose tissue |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 45.2 kDa |
| ⓘ | Localization | Nucleus |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Rat |
Section 1
Real Curated FOXJ1 Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Bronchus |
| Gel % | 12-15% |
| Load | 20-30 µg total protein per lane |
| Transfer | Semi-dry, standard transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | A04942-3 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected FOXJ1 Western Blot Band Size?
Use the product-observed 45.2 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 45.2 kDa | Matches the authoritative product WB observation. |
| 45.2 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected FOXJ1 appearancePlan around 45.2 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 45.2 kDa; use this as the primary experimental expectation. |
| Calculated mass | 45.2 kDa from UniProt Q92949; retain as context. |
| Gel selection | 12-15%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with A04942-3. |
| Situation | Likely cause | Next action |
|---|---|---|
| 45.2 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing FOXJ1 band
A04942-3 · FOXJ1
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for FOXJ1 Western blot
🧪Use Fallopian tube as the first positive-control candidate and Adipose tissue as the HPA Not detected negative candidate.
Positive control: Fallopian tube (High)
Negative control: Adipose tissue (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for FOXJ1
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Fallopian tube | Reported tissue cells | High | Protein (HPA) | HPA → |
| Bronchus | Reported tissue cells | High | Protein (HPA) | HPA → |
| Endometrium | Reported tissue cells | High | Protein (HPA) | HPA → |
| Efferent ducts | Reported tissue cells | Medium | Protein (HPA) | HPA → |
Section 3
Advanced FOXJ1 Western Blot Tips
Deeper troubleshooting and optimisation questions for FOXJ1, answered from its protein features.
Which band should guide the blot?
Use 45.2 kDa, the observation attached to the authoritative A04942-3 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 45.2 kDa expectation.
Which positive control should I start with?
Start with Fallopian tube, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Adipose tissue as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 12-15% consistently across the quick facts, protocol table, and poster.
What transfer method to use for FOXJ1 Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should A04942-3 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
FOXJ1 Western Blot Reagents
Human/Rat-reactive FOXJ1 Western blot reagents with authoritative product imagery.
Real WB data
Anti-FOXJ1 Antibody Picoband®
Cat # A04942-3
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt Q92949
- Human Protein Atlas — FOXJ1
- A04942-3 product record
- PMC6884487 — Altered generation of ciliated cells in chronic obstructive pulmonary disease (Scientific reports, 2019)
- PMC9243675 — FoxJ1 inhibits African swine fever virus replication and viral S273R protein decreases the expression of FoxJ1 to impair its antiviral effect (Virologica Sinica, 2022)
- PMC3710277 — RFX3 modulation of FOXJ1 regulation of cilia genes in the human airway epithelium (Respiratory research, 2013)