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- Table of Contents
SOCS3 · Western blot design guide
SOCS3 Western Blot Planning Guide
Plan a SOCS3 Western blot around the catalog-observed 24.8 kDa band, image-backed A00274-2 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
SOCS3 Western Blot Experimental Design Guide
Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 24.8 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 12-15% | |
| Positive control | Fallopian tube | |
| Negative control | Lymph node |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 24.8 kDa |
| ⓘ | Localization | Not reported |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse / Rat |
Section 1
Real Curated SOCS3 Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Fallopian tube |
| Gel % | 12-15% |
| Load | 20-30 µg total protein per lane |
| Transfer | Semi-dry, short transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | A00274-2 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected SOCS3 Western Blot Band Size?
Use the product-observed 24.8 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 24.8 kDa | Matches the authoritative product WB observation. |
| 24.8 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected SOCS3 appearancePlan around 24.8 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 24.8 kDa; use this as the primary experimental expectation. |
| Calculated mass | 24.8 kDa from UniProt O14543; retain as context. |
| Gel selection | 12-15%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with A00274-2. |
| Situation | Likely cause | Next action |
|---|---|---|
| 24.8 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing SOCS3 band
A00274-2 · SOCS3
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for SOCS3 Western blot
🧪Use Fallopian tube as the first positive-control candidate and Lymph node as the HPA Not detected negative candidate.
Positive control: Fallopian tube (High)
Negative control: Lymph node (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for SOCS3
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Fallopian tube | Reported tissue cells | High | Protein (HPA) | HPA → |
| Gallbladder | Reported tissue cells | High | Protein (HPA) | HPA → |
| Adrenal gland | Reported tissue cells | Medium | Protein (HPA) | HPA → |
| Bone marrow | Reported tissue cells | Medium | Protein (HPA) | HPA → |
Section 3
Advanced SOCS3 Western Blot Tips
Deeper troubleshooting and optimisation questions for SOCS3, answered from its protein features.
Which band should guide the blot?
Use 24.8 kDa, the observation attached to the authoritative A00274-2 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 24.8 kDa expectation.
Which positive control should I start with?
Start with Fallopian tube, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Lymph node as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 12-15% consistently across the quick facts, protocol table, and poster.
What transfer method to use for SOCS3 Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should A00274-2 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
Compare only the supplied target-verified records: PMC3196771, PMC9940702, PMC7408579.
Boster reagents
SOCS3 Western Blot Reagents
Human/Mouse/Rat-reactive SOCS3 Western blot reagents with authoritative product imagery.
Real WB data
Anti-SOCS3 Antibody Picoband®
Cat # A00274-2
Real WB data
Anti-SOCS3 Antibody Picoband®
Cat # PA1707
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt O14543
- Human Protein Atlas — SOCS3
- A00274-2 product record
- PMC3196771 — Zoledronic acid inhibits macrophage SOCS3 expression and enhances cytokine production.
- PMC9940702 — ATP citrate lyase links increases in glycolysis to diminished release of vesicular suppressor of cytokine signaling 3 by alveolar macrophages.
- PMC7408579 — Oxidative Inactivation of the Proteasome Augments Alveolar Macrophage Secretion of Vesicular SOCS3.