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- Table of Contents
SMARCA4 Antibodies
AND SMARCA4 ELISA kits, SMARCA4 Proteins
Many biological assays use antibodies to detect SMARCA4, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with SMARCA4 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop SMARCA4 antibodies...
Designing an IHC experiment for SMARCA4? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.
Open the SMARCA4 IHC GuideSMARCA4 Infographic by Boster Bio
All SMARCA4 Products
Product
Figures
Specificity
Applications
Price
Human, Mouse, Rat
ELISA, IF, IHC, ICC, WB
$370
Cat # A00223-1
100 μg/vial
Cat # A00223-1
100 μg/vial
Human, Mouse, Rat
Flow Cytometry, IHC, WB
$370
Cat # M00223-1
100 μg/vial
Cat # M00223-1
100 μg/vial
Human, Mouse, Rat
IP, IF, IHC, ICC, WB
$370
Cat # M00223
100 μl
Cat # M00223
100 μl
Human, Mouse, Rat
ELISA, IF, IHC-P, WB
$481
Cat # A00223
0.1 mg
Cat # A00223
0.1 mg
Human, Rat
ELISA, IP, IF, IHC, WB
$146
Cat # M00223-3
20μL, 40μL, 100μL, 200μL
Cat # M00223-3
20μL, 40μL, 100μL, 200μL
SMARCA4 Overview
Facts about Transcription activator BRG1.
Transcription activator BRG1 (SMARCA4)
Part of SWI/SNF chromatin remodeling complexes that carry out key enzymatic actions, changing chromatin structure by changing DNA-histone contacts within a nucleosome within an ATP-dependent manner. Part of the CREST-BRG1 complicated, a multiprotein complex that regulates promoter activation by orchestrating the calcium-dependent discharge of a repressor complex and the recruitment of an activator complex.
At the exact same time, there's increased recruiting of CREBBP to the promoter with a CREST- dependent mechanism, which contributes to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression includes the release of HDAC1 and recruiting of CREBBP.
Gene Information
| Human | |
|---|---|
| Gene Name: | SMARCA4 |
| Uniprot: | P51532 |
| Entrez: | 6597 |
Family
| Belongs to: |
|---|
| SNF2/RAD54 helicase family |
Alternative Names
ATP-dependent helicase SMARCA4; BAF190; BAF190A; brahma protein-like 1; Brg1; BRG1-associated factor 190A; BRG1SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A member 4; BRM/SWI2-related gene 1; EC 3.6.1; EC 3.6.4.-; FLJ39786; global transcription activator homologous sequence; homeotic gene regulator; hSNF2b; Mitotic growth and transcription activator; nuclear protein GRB1; Protein brahma homolog 1; Protein BRG-1; RTPS2; SMARCA4; SNF2; SNF2B; SNF2-beta; SNF2L4; SNF2LB; SNF2-like 4; subfamily a, member 4; sucrose nonfermenting-like 4; SWI/SNF related, matrix
Molecular Weight
Mass (kDA):
184.646 kDA
Genomic Context
| Human | |
|---|---|
| Location: | 19p13.2 |
| Sequence: | 19; NC_000019.10 (10960997..11062277) |
Tissue Specificity
Colocalizes with ZEB1 in E-cadherin-negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas (at protein level).
Subcellular Localizations
Nucleus. Colocalizes with long non-coding RNA Evf2 in nuclear RNA clouds.
Diseases
- Neoplasms
- Malignant Neoplasms
- Carcinoma
- Cell Transformation, Neoplastic
- Retinoblastoma
- Mammary Neoplasms
- Malignant Paraganglionic Neoplasm
- Malignant Neoplasm Of Lung
- Adenocarcinoma
- Hyperplasia
- Growth Arrest
- Lung Neoplasms
- Malignant Neoplasm Of Breast
Interacting Proteins
- ARID1A
- ARID1B
- ARID2
- H2AFX
- KDM1A
- NR2E1
- Nr3c1
- PBRM1
- RBPJ
- REST
Pathways
- Chromatin Remodeling
- Cell Cycle
- Atp-dependent Chromatin Remodeling
- Methylation
- Cell Proliferation
- Dna Repair
- Cell Growth
- Histone Acetylation
- Regulation Of Gene Expression
- Cell Differentiation
- Rna Interference
- Cell Cycle Arrest
- Dna Methylation
PTMs
- Acetylation
- Methylation
- Phosphorylation
- Deacetylation
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/SMARCA4
bosterbio.com/bosterbio-gene-info-cards/SMARCA4
References
PMID: 8232556 by Khavari P.A., et al. BRG1 contains a conserved domain of the SWI2/SNF2 family necessary for normal mitotic growth and transcription.
PMID: 8208605 by Chiba H., et al. Two human homologues of Saccharomyces cerevisiae SWI2/SNF2 and Drosophila brahma are transcriptional coactivators cooperating with the estrogen receptor and the retinoic acid receptor.