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Product Info Summary
| SKU: | PROTP06684 |
|---|---|
| Size: | 2ug, 10ug, 1mg |
| Origin Species: | Mouse |
| Source: | Escherichia coli |
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Product info
Product Name
Complement Component C5a Mouse Recombinant Protein
View all C5 recombinant proteins
SKU/Catalog Number
PROTP06684
Size
2ug, 10ug, 1mg
Description
C5a Mouse Recombinant produced in E. coli is a single, non-glycosylated, Polypeptide chain containing 77 amino acids and having a molecular mass of 9kDa. The Mouse C5a is purified by proprietary chromatographic techniques.
Storage & Handling
Lyophilized Mouse C5a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Mouse C5a should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Cite This Product
Complement Component C5a Mouse Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP06684)
Form
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Mouse C5a was lyophilized from a concentrated (1mg/ml) solution in 20mM PB, pH 7.5 and 350mM NaCl.
Purity
Greater than 95.0% as determined by (a) Analysis by RP-HPLC and (b) Analysis by SDS-PAGE.
Predicted MW
188.305kDa
Reconstitution
It is recommended to reconstitute the lyophilized Mouse C5a in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Amino Acid Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Asn-Leu-His-Leu-Leu.
Biological Activity
The ED50 of Recombinant Mouse C5a as determined by its ability to induce N-acetyl-b-D-glucosaminidase release from differentiated U937 human histiocytic lymphoma cells was 5-20ng/ml.
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Assay dilution & Images
Reconstitution
It is recommended to reconstitute the lyophilized Mouse C5a in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Validation Images & Assay Conditions
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Recombinant protein fun image
Protein Target Info & Infographic
Gene/Protein Information For C5 (Source: Uniprot.org, NCBI)
Gene Name
C5
Full Name
Complement C5
Weight
188.305kDa
Alternative Names
C5; Complement Component C5/C5a C5 C5Da, C5b, CPAMD4, ECLZB, C5 complement C5 complement C5|C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4|C5a anaphylatoxin|anaphylatoxin C5a analog|complement component 5|prepro-C5
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on C5, check out the C5 Infographic
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In this infographic, you will see the following information for C5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].
Specific Publications For Complement Component C5a Mouse Recombinant Protein (PROTP06684)
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