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Product Info Summary
| SKU: | EK1455 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, milk and urine. |
Product info
Product Name
Human APOE/Apolipoprotein E ELISA Kit PicoKine®
SKU/Catalog Number
EK1455
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human APOE/Apolipoprotein E ELISA Kit PicoKine™ (96 Tests). Quantitate Human APOE in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, milk and urine. Sensitivity: 50pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human APOE/Apolipoprotein E ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1455)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: K19-H317
Sensitivity
<50 pg/ml
Assay Range
3.12 ng/ml - 200 ng/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1455 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1455 is reactive to APOE in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, milk and urine.
Application Guarantee
EK1455 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of APOE
Apolipoprotein E (APOE) is a class of apolipoprotein found in the chylomicron and Intermediate-density lipoprotein (IDLs) that is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. In peripheral tissues, APOE is primarily produced by the liver and macrophages, and mediates cholesterol metabolism in an isoform- dependent manner. In the central nervous system, APOE is mainly produced by astrocytes, and transports cholesterol to neurons via APOE receptors, which are members of the low density lipoprotein receptor gene family. This protein is involved in Alzheimer’s disease and cardiovascular disease.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1455-CAP | Anti-Human APOE Pre-coated 96-well strip microplate | 1 |
| EK1455-ST | Human APOE Standard | 2 vials, 200 ng/tube |
| EK1455-DA | Human APOE Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (ng/ml) | 0 | 3.12 | 6.25 | 12.5 | 25 | 50 | 100 | 200 |
| O.D. | 0.010 | 0.073 | 0.138 | 0.243 | 0.484 | 0.907 | 1.475 | 2.004 |
Data Example Images
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Human APOE PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect APOE, Dilution ratio of 1:1000, concentration in serum and plasma is around 20-40 ug/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human APOE/Apolipoprotein E ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 7334 | 30860 | 101114 | 7480 | 33927 | 97046 |
| Standard deviation | 506.04 | 2191.06 | 7886.89 | 598.4 | 2917.72 | 7957.77 |
| CV (%) | 6.9% | 7.1% | 7.8% | 8% | 8.6% | 8.2% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of APOE in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 7334 | 7205 | 6656 | 6991 | 7046 | 256.58 | 3.6% |
| Sample 2 | 30860 | 28598 | 30251 | 27221 | 29232 | 1426.08 | 4.8% |
| Sample 3 | 101114 | 104676 | 102789 | 88986 | 99391 | 6138.2 | 6.1% |
Protein Target Info & Infographic
Gene/Protein Information For APOE (Source: Uniprot.Org, NCBI)
Gene Name
APOE
Full Name
Apolipoprotein E
Weight
36.154kDa
Superfamily
apolipoprotein A1/A4/E family
Alternative Names
APOE; Apo-E; Apolipoprotein E; apolipoprotein E3; late onset); MGC1571 APOE AD2, APO-E, ApoE4, LDLCQ5, LPG apolipoprotein E apolipoprotein E|apolipoprotein E3
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on APOE, check out the APOE Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for APOE: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human APOE/Apolipoprotein E ELISA Kit PicoKine® (EK1455)
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No publications found for EK1455
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Customer Reviews
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Customer Q&As
Have a question?
Find answers in Q&As, reviews.
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9 Customer Q&As for Human APOE/Apolipoprotein E ELISA Kit PicoKine®
Question
Q: Can APOE ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
M. Jackson
Verified customer
Asked: 2019-05-06
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-05-06
Question
Q: which procedure should I follow in order to thaw whole blood sample for APOE ELISA after freezing?
Verified Customer
Verified customer
Asked: 2019-03-22
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test APOE for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2019-03-22
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2018-07-13
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-07-13
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2018-07-02
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-07-02
Question
Q: What is the optimal O.D. value for APOE ELISA kit? I used your APOE ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain APOE even though the O.D. values are not very high?
H. Lopez
Verified customer
Asked: 2018-04-06
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this APOE ELISA kit to have sensitivity of 50pg/ml, that means the minimum amount of APOE that can be declared/interpreted as positive by the above standard is 50pg/ml.
Boster Scientific Support
Answered: 2018-04-06
Question
Q: is it okay to use citrate plasma as samples in Human APOE Picokine® ELISA Kit (Catalog # EK1455)?
Verified Customer
Verified customer
Asked: 2017-09-21
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of APOE causing disruption of its protein structure. APOE may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the APOE ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2017-09-21
Question
A customer wanted to know if EK1455 is cross-reactive with Monkey or other non-human primate species also?
Verified Customer
Verified customer
Asked: 2017-06-05
Answer
We haven't experimentally validate the cross-reactivity with Monkey or other non-human primate species. Sequence of the antibodies in the kit is K19-H317. Please see the sequence homology between human and Monkey. https://www.uniprot.org/blast/uniprot/B201904306746803381A1F0E0DB47453E0216320D0152AEL
Boster Scientific Support
Answered: 2017-06-05
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of APOE in Human plasma? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2017-02-11
Answer
A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the APOE ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2017-02-11
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2017-01-08
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2017-01-08
