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Product Info Summary
| SKU: | FEK0707 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants and serum. |
| Assay Time: | 120 Minutes |
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Product info
Introduction
Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.
Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol
Product Name
Human B7-1/CD80 PicoKine® Quick ELISA Kit
SKU/Catalog Number
FEK0707
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human B7-1/CD80 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CD80 in cell culture supernatants and serum. Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human B7-1/CD80 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0707)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: V35-N242
Sensitivity
<10 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of FEK0707 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK0707 is reactive to CD80 in Human samples
Validated Sample Types
cell culture supernatants and serum.
Application Guarantee
FEK0707 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of B7-1/CD80
The protein CD80 (Cluster of Differentiation 80) is a molecule found on activated B cells and monocytes which provides a costimulatory signal necessary for T cell activation and survival. It is also known as B7-1. The cDNA for B7-1 predicts a type I membrane protein, i.e., one synthesized with a signal peptide that is cleaved upon translocation across the endoplasmic membrane. The protein is predicted to contain 2 extracellular domains structurally similar to those of Ig, a hydrophobic transmembrane region, and a short cytoplasmic domain. The CD80 and CD86 genes encode B7-1 and B7-2, respectively, which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types. B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4.
Kit Components
| Catalog number | Description | Quantity |
|---|---|---|
| FEK0707-CAP | 96-well plate precoated with anti-Human B7-1/CD80 antibody | 1 |
| EK0707-ST | lyophilized recombinant Human B7-1/CD80 standard | 10 ng/tube |
| FEK0707-DA | Biotinylated anti-Human B7-1/CD80 detection antibody (50x) | 100 ul |
| - | Avidin-Biotin-Peroxidase Complex (30x) | 400 µl |
| AR1106-1 | Sample diluent | 30 ml |
| AR1106-2 | Antibody diluent | 12 ml |
| - | Avidin-Biotin-Peroxidase Diluent | 12 ml |
| AR1106-5 | Wash Buffer (25x) | 20 ml |
| AR1104 | Color Developing Reagent (TMB) | 10 ml |
| AR1105 | Stop Solution | 10 ml |
| PLA-SEA | Plate Sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.006 | 0.06 | 0.129 | 0.256 | 0.442 | 0.829 | 1.384 | 1.979 |
Data Example Images
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Human B7-1/CD80 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 162 | 445 | 1656 | 163 | 425 | 1791 |
| Standard deviation | 7.29 | 34.71 | 69.55 | 7.98 | 34 | 100.29 |
| CV (%) | 4.5% | 7.8% | 4.2% | 4.9% | 8% | 5.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of B7-1/CD80 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 162 | 138 | 164 | 139 | 150 | 12.27 | 8.1% |
| Sample 2 | 445 | 495 | 515 | 457 | 478 | 28.23 | 5.9% |
| Sample 3 | 1656 | 1667 | 1402 | 1560 | 1571 | 106.21 | 6.7% |
Protein Target Info & Infographic
Gene/Protein Information For CD80 (Source: Uniprot.Org, NCBI)
Uniprot ID
P33681
Gene ID
941
Gene Name
CD80
Full Name
T-lymphocyte activation antigen CD80
Weight
33.048kDa
Alternative Names
Activation B7-1 antigen; B7; B71; B7-1; BB1; CD28LG1; CD28LGB7-1 antigen); CD80 antigen; CD80 molecule; CD80; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; T-lymphocyte activation antigen CD80 CD80 B7, B7-1, B7.1, BB1, CD28LG, CD28LG1, LAB7 CD80 molecule T-lymphocyte activation antigen CD80|B-lymphocyte activation antigen B7|CD80 antigen (CD28 antigen ligand 1, B7-1 antigen)|CTLA-4 counter-receptor B7.1|activation B7-1 antigen|costimulatory factor CD80|costimulatory molecule variant IgV-CD80
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CD80, check out the CD80 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CD80: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human B7-1/CD80 PicoKine® Quick ELISA Kit (FEK0707)
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No publications found for FEK0707
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Human B7-1/CD80 PicoKine® Quick ELISA Kit
Question
Is FEK0707 suitable for evaluation of urinary CD80 level in children with primary nephrotic syndrome and its relation with response to corticosteroids?
Verified customer
Asked: 2019-11-04
Answer
Our lab has not worked with urine samples using the Human B7-1/CD80 PicoKine™ Fast ELISA Kit (FEK0707). We cannot guarantee the kits would work for urine samples and we suggest to run a pilot test if you want to try. In theory the ELISA kit will work for all sample types if the target protein is present at a level that falls within the linear range of the ELISA kit detection range. We guarantee the kit to work on the sample types that we have tested. For sample types that we have not tested for, we suggest to run pilot experiments to decide the optimal sample dilution.
Boster Scientific Support
Answered: 2019-11-05
