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Product Info Summary
| SKU: | EK0744 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human C-MET/HGFR ELISA Kit PicoKine®
View all HGFR/c-MET ELISA kits
SKU/Catalog Number
EK0744
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human C-MET/HGFR ELISA Kit PicoKine™ (96 Tests). Quantitate Human MET in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human C-MET/HGFR ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0744)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: E25-R307 (alpha) & S308-T932 (beta)
Sensitivity
<5 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0744 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0744 is reactive to MET in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0744 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of HGFR/c-MET
C-Met (MET or MNNG HOS Transforming gene) is a proto-oncogene that encodes a protein known as hepatocyte growth factor receptor (HGFR). MET proto-oncogene has a total length of 125,982 bp, and it is located in the 7q31 locus of chromosome 7. MET is a membrane receptor that is essential for embryonic development and wound healing. Activation of MET triggers mitogenesis, and morphogenesis.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0744-CAP | Anti-Human MET Pre-coated 96-well strip microplate | 1 |
| EK0744-ST | Human MET Standard | 2 vials, 10 ng/tube |
| EK0744-DA | Human MET Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.036 | 0.081 | 0.118 | 0.236 | 0.340 | 0.552 | 1.035 | 1.955 |
Data Example Images
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Human C-MET/HGFR PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human C-MET/HGFR ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 210 | 601 | 1336 | 204 | 644 | 1384 |
| Standard deviation | 8.61 | 31.25 | 84.16 | 10.81 | 39.28 | 95.49 |
| CV (%) | 4.1% | 5.2% | 6.1% | 5.3% | 6.1% | 6.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of HGFR/c-MET in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 210 | 196 | 181 | 199 | 196 | 10.35 | 5.2% |
| Sample 2 | 601 | 676 | 644 | 633 | 638 | 26.8 | 4.2% |
| Sample 3 | 1336 | 1534 | 1527 | 1377 | 1443 | 88.23 | 6.1% |
Protein Target Info & Infographic
Gene/Protein Information For MET (Source: Uniprot.Org, NCBI)
Gene Name
MET
Full Name
Hepatocyte growth factor receptor
Weight
155.541kDa
Superfamily
protein kinase superfamily
Alternative Names
AUTS9; cMET; c-MET; EC 2.7.10; EC 2.7.10.1; hepatocyte growth factor receptor; HGF R; HGF receptor; HGF/SF receptor; HGFR; Met (c-Met); met proto-oncogene (hepatocyte growth factor receptor); met proto-oncogene tyrosine kinase; MET; oncogene MET; Proto-oncogene c-Met; RCCP2; Scatter factor receptor; SF receptor; Tyrosine-protein kinase Met MET AUTS9, DFNB97, HGFR, RCCP2, c-Met MET proto-oncogene, receptor tyrosine kinase hepatocyte growth factor receptor|HGF receptor|HGF/SF receptor|SF receptor|proto-oncogene c-Met|scatter factor receptor|tyrosine-protein kinase Met
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on MET, check out the MET Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MET: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human C-MET/HGFR ELISA Kit PicoKine® (EK0744)
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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5 Customer Q&As for Human C-MET/HGFR ELISA Kit PicoKine®
Question
Q: can I use citrate plasma as samples in Human HGFR Picokine® ELISA Kit (Catalog # EK0744)?
Verified Customer
Verified customer
Asked: 2020-05-12
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of HGFR causing disruption of its protein structure. HGFR may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the HGFR ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-05-12
Question
Q: how to proceed with the analysis of ELISA data? I have obtained HGFR level in plasma.
Verified Customer
Verified customer
Asked: 2019-11-01
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-11-01
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification HGFR using the EK0744 Human HGFR Picokine® ELISA Kit?
S. Zhou
Verified customer
Asked: 2019-02-28
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2019-02-28
Question
Q: how to thaw whole blood sample for HGFR ELISA after freezing?
R. Patel
Verified customer
Asked: 2019-02-15
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test HGFR for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2019-02-15
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-12-21
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-12-21
