Met (Phospho-Tyr1234) Colorimetric Cell-Based ELISA Kit

HGFR/c-MET ELISA kit, phospho-specific, cell-based

The Met (Phospho-Tyr1234) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Met (Phospho-Tyr1234) protein expression profile in cells. The kit can be used for measuring the relative amounts of Met (Phospho-Tyr1234) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Met (Phospho-Tyr1234).

Product Info Summary

SKU: EKC2461
Size: 1 kit, containing two 96-well plates and all necessary reagents
Reactive Species: Human, Mouse, Rat
Application: ELISA

Product Name

Met (Phospho-Tyr1234) Colorimetric Cell-Based ELISA Kit

See all HGFR/c-MET products

SKU/Catalog Number

EKC2461

Size

1 kit, containing two 96-well plates and all necessary reagents

Description

The Met (Phospho-Tyr1234) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Met (Phospho-Tyr1234) protein expression profile in cells. The kit can be used for measuring the relative amounts of Met (Phospho-Tyr1234) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Met (Phospho-Tyr1234).

Storage & Handling

Store at 4°C for up to 6 months.

Cite This Product

Met (Phospho-Tyr1234) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2461)

Clonality of Antibodies

See Datasheet for details

Assay Range

> 5000 cells/well

Reactive Species

EKC2461 is reactive to MET in Human, Mouse, Rat samples

Application Guarantee

EKC2461 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 2 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-MET (Phospho-specific) Antibody (Rabbit Polyclonal) 60 µl (100x) Red
100x Anti-MET Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 12ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 12ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 12ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

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Gene/Protein Information For MET (Source: Uniprot.Org, NCBI)

Gene Name

MET

Full Name

Hepatocyte growth factor receptor

Weight

155.541kDa

Superfamily

protein kinase superfamily

Alternative Names

AUTS9; cMET; c-MET; EC 2.7.10; EC 2.7.10.1; hepatocyte growth factor receptor; HGF R; HGF receptor; HGF/SF receptor; HGFR; Met (c-Met); met proto-oncogene (hepatocyte growth factor receptor); met proto-oncogene tyrosine kinase; MET; oncogene MET; Proto-oncogene c-Met; RCCP2; Scatter factor receptor; SF receptor; Tyrosine-protein kinase Met MET AUTS9, DFNB97, HGFR, RCCP2, c-Met MET proto-oncogene, receptor tyrosine kinase hepatocyte growth factor receptor|HGF receptor|HGF/SF receptor|SF receptor|proto-oncogene c-Met|scatter factor receptor|tyrosine-protein kinase Met

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on MET, check out the MET Infographic

MET infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for MET: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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1 Customer Q&As for Met (Phospho-Tyr1234) Colorimetric Cell-Based ELISA Kit

Question

Does EKC2461 contain any proteins, recombinant antibody, cells and cell lysate?

Verified customer

Asked: 2021-02-11

Answer

The Met (Phospho-Tyr1234) Colorimetric Cell-Based ELISA Kit (EKC2461) does not contain protein, recombinant protein, cells or cell lysates.

Boster Scientific Support

Answered: 2021-02-11

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