Product Info Summary
SKU: | EK1426 |
---|---|
Size: | 96 wells/kit, with removable strips. |
Reactive Species: | Human |
Application: | ELISA |
Sample Types: | cell culture supernatantsand serum. |
Product info
Product Name
Human Caspase 8/CASP8 ELISA Kit PicoKine®
SKU/Catalog Number
EK1426
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Caspase 8/CASP8 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CASP8 in cell culture supernatantsand serum. Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Caspase 8/CASP8 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1426)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: S217-D384 & L385-D479
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1426 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1426 is reactive to CASP8 in Human samples
Validated Sample Types
cell culture supernatantsand serum.
Application Guarantee
EK1426 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Caspase-8
Caspase-8 is a caspase protein, encoded by the CASP8 gene. It is mapped to human chromosome 2q33-q34 and mouse chromosome 1B-proximal C. This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. And this protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.
Kit Components
Catalog Number | Description | Quantity |
---|---|---|
EK1426-CAP | Anti-Human CASP8 Pre-coated 96-well strip microplate | 1 |
EK1426-ST | Human CASP8 Standard | 2 vials, 10 ng/tube |
EK1426-DA | Human CASP8 Biotinylated antibody (100x) | 100ul |
AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
AR1106-1 | Sample Diluent | 30ml |
AR1106-2 | Antibody Diluent | 12ml |
AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop Solution | 10ml |
AR1106-5 | Wash Buffer (25x) | 20ml |
PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.062 | 0.091 | 0.153 | 0.216 | 0.335 | 0.636 | 1.112 | 1.758 |
Data Example Images
Click image to see more details
Human Caspase 8 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Caspase-8, Dilution ratio of 1:1, concentration in serum is around 120 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Caspase 8/CASP8 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 89 | 257 | 911 | 90 | 251 | 854 |
Standard deviation | 6.49 | 16.44 | 46.46 | 6.57 | 20.58 | 46.11 |
CV (%) | 6.4% | 6.4% | 5.4% | 7.3% | 8.2% | 5.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Caspase-8 in ELISA kits from four different production batches/lots.
Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
---|---|---|---|---|---|---|---|
Sample 1 | 89 | 99 | 100 | 96 | 96 | 4.3 | 4.4% |
Sample 2 | 257 | 285 | 267 | 304 | 278 | 17.93 | 6.4% |
Sample 3 | 911 | 915 | 904 | 795 | 881 | 49.95 | 5.6% |
Protein Target Info & Infographic
Gene/Protein Information For CASP8 (Source: Uniprot.Org, NCBI)
Gene Name
CASP8
Full Name
Caspase-8
Weight
55.391kDa
Superfamily
peptidase C14A family
Alternative Names
AIS; androgen receptor; CASP8; Caspase8; Caspase-8; DHTRTFM; Dihydrotestosterone receptorHYSP1; HUMARA; Mch5; NR3C4KD; Nuclear receptor subfamily 3 group C member 4; SMAX1SBMA; spinal and bulbar muscular atrophy CASP8 ALPS2B, CAP4, Casp-8, FLICE, MACH, MCH5 caspase 8 caspase-8|FADD-homologous ICE/CED-3-like protease|FADD-like ICE|ICE-like apoptotic protease 5|MACH-alpha-1/2/3 protein|MACH-beta-1/2/3/4 protein|MORT1-associated ced-3 homolog|apoptotic cysteine protease|apoptotic protease Mch-5|caspase 8, apoptosis-related cysteine peptidase|caspase 8, apoptosis-related cysteine protease
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CASP8, check out the CASP8 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CASP8: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Caspase 8/CASP8 ELISA Kit PicoKine® (EK1426)
Hello CJ!
EK1426 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Tang D, Gao J, Wang S, Yuan Z, Ye N, Chong Y, Xu C, Jiang X, Li B, Yin W, Miao Y, Wang D, Jiang K. Tumour Biol. 2015 Jul;36(7):5617-26. Doi: 10.1007/S13277-015-3233-5. Epub 2015 Mar 1. Apoptosis And Anergy Of T Cell Induced By Pancreatic Stellate ...
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
8 Customer Q&As for Human Caspase 8/CASP8 ELISA Kit PicoKine®
Question
Q: how to proceed with the analysis of ELISA data? I have performed Caspase-8 level in cell culture supernatesand serum.
A. Bhasin
Verified customer
Asked: 2020-11-14
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-11-14
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2020-05-17
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-05-17
Question
Q: is it okay to use heparin plasma as samples in Human Caspase-8 Picokine® ELISA Kit (Catalog # EK1426)?
E. Parker
Verified customer
Asked: 2020-04-07
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of Caspase-8 causing disruption of its protein structure. Caspase-8 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the Caspase-8 ELISA, treating samples with various anticoagulants and decided that / can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-04-07
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2018-12-07
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-12-07
Question
Q: Can Caspase-8 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2018-11-15
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-11-15
Question
Q: how to thaw whole blood sample for Caspase-8 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2018-03-13
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Caspase-8 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2018-03-13
Question
Q: if the enzyme conjugated Caspase-8 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Caspase-8 antigen?
Verified Customer
Verified customer
Asked: 2018-02-26
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-02-26
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
M. Hernandez
Verified customer
Asked: 2017-10-27
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2017-10-27