Click image to see more details
Product Info Summary
| SKU: | EK1527 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human CD48/Slamf2 ELISA Kit PicoKine®
View all CD48/SLAMF2 ELISA kits
SKU/Catalog Number
EK1527
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CD48/Slamf2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CD48 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 12pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CD48/Slamf2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1527)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: Q27-S220
Sensitivity
<12 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1527 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1527 is reactive to CD48 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1527 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CD48/SLAMF2
CD48 antigen (Cluster of Differentiation 48), also known as BLAST-1 or SLAMF2, is a protein that in humans is encoded by the CD48 gene. CD48 is a member of the CD2 subfamily of the immunoglobulin superfamily (IgSF) which includes SLAM (signaling lymphocyte activation molecules) proteins, such as CD84, CD150, CD229 and CD244. It is mapped to 1q23.3. CD48 is found on the surface of lymphocytes and other immune cells, dendritic cells and endothelial cells, and participates in activation and differentiation pathways in these cells. The encoded protein does not have a transmembrane domain, however, but is held at the cell surface by a GPI anchor via a C-terminal domain which maybe cleaved to yield a soluble form of the receptor.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1527-CAP | Anti-Human CD48 Pre-coated 96-well strip microplate | 1 |
| EK1527-ST | Human CD48 Standard | 2 vials, 10 ng/tube |
| EK1527-DA | Human CD48 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
Submit A Review
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.040 | 0.131 | 0.202 | 0.350 | 0.633 | 0.975 | 1.412 | 1.785 |
Data Example Images
Click image to see more details
Human CD48 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CD48/SLAMF2, Dilution ratio of 1:20, concentration in serum and plasma is 8-15 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CD48/Slamf2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 113 | 615 | 2155 | 117 | 667 | 2219 |
| Standard deviation | 5.53 | 46.12 | 129.3 | 6.66 | 58.69 | 157.54 |
| CV (%) | 4.9% | 7.5% | 6% | 5.7% | 8.8% | 7.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CD48/SLAMF2 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 113 | 100 | 101 | 104 | 104 | 5.12 | 4.9% |
| Sample 2 | 615 | 601 | 670 | 606 | 623 | 27.59 | 4.4% |
| Sample 3 | 2155 | 1979 | 1842 | 1829 | 1951 | 131.49 | 6.7% |
Protein Target Info & Infographic
Gene/Protein Information For CD48 (Source: Uniprot.Org, NCBI)
Gene Name
CD48
Full Name
CD48 antigen
Weight
27.683kDa
Alternative Names
BCM1 surface antigen; BCM1; BCM1Leukocyte antigen MEM-102; BLAST; BLAST1; BLAST1TCT.1; B-lymphocyte activation marker BLAST-1; CD48 antigen (B-cell membrane protein); CD48 antigen; CD48 molecule; CD48; hCD48; mCD48; MEM-102; SLAMF2 CD48 BCM1, BLAST, BLAST1, MEM-102, SLAMF2, hCD48, mCD48 CD48 molecule CD48 antigen|B-lymphocyte activation marker BLAST-1|BCM1 surface antigen|CD48 antigen (B-cell membrane protein)|SLAM family member 2|TCT.1|leukocyte antigen MEM-102|signaling lymphocytic activation molecule 2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CD48, check out the CD48 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CD48: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CD48/Slamf2 ELISA Kit PicoKine® (EK1527)
Hello CJ!
No publications found for EK1527
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Human CD48/Slamf2 ELISA Kit PicoKine®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Human CD48/Slamf2 ELISA Kit PicoKine®
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
7 Customer Q&As for Human CD48/Slamf2 ELISA Kit PicoKine®
Question
Q: What is the optimal O.D. value for CD48 ELISA kit? I used your CD48 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain CD48 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2020-08-31
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CD48 ELISA kit to have sensitivity of 12pg/ml, that means the minimum amount of CD48 that can be declared/interpreted as positive by the above standard is 12pg/ml.
Boster Scientific Support
Answered: 2020-08-31
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of CD48 in Human serum? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
M. Miller
Verified customer
Asked: 2020-06-03
Answer
A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CD48 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-06-03
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
G. Wright
Verified customer
Asked: 2020-04-28
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-04-28
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification CD48 using the EK1527 Human CD48 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2020-01-10
Question
Q: if the enzyme conjugated CD48 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CD48 antigen?
Verified Customer
Verified customer
Asked: 2019-04-06
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-04-06
Question
Q: how can I thaw whole blood sample for CD48 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2018-05-13
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CD48 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2018-05-13
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2014-03-26
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2014-03-26
