Human CXCL7 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

PPBP ELISA antibody pairs, Human, for 5 plates

Human CXCL7 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human PPBP in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

Product Info Summary

SKU: EZ0729
Size: For 5 plates, 96 wells each
Reactive Species: Human
Application: ELISA

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Product Name

Human CXCL7 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

See all PPBP products

SKU/Catalog Number

EZ0729

Size

For 5 plates, 96 wells each

*Question: How many samples can I assay/run in this kit?

Description

Human CXCL7 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human PPBP in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human CXCL7 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ0729)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A59-D128

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EZ0729 is reactive to PPBP in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

Application Guarantee

EZ0729 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog number Description Quantity Storage of opened/reconstituted material
EZ0729-CA Rabbit anti- human PPBP polyclonal antibody (Capture Antibody) 500 µl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit.
EZ0729-DA Biotinylated goat anti- human PPBP polyclonal antibody (Detection Antibody) 500 µl, 2.5 µg/mL (recommended dilution 1:100)
AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 µl (recommended dilution 1:100)
EK0729-ST Lyophilized recombinant human PPBP standard 10 ng/tube×3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • 1. Microplate reader in standard size.
  • 2. Automated plate washer.
  • 3. Incubator.
  • 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • 5. Clean tubes and Eppendorf tubes.
  • 6. 96 well microplate (Cat# AR1100)
  • 7. Plate Sealers.
  • 8. Capture Antibody Diluent: PBS.
  • 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
  • 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
  • 11. Stop Solution: 2 N H2SO4 (Cat# AR1105)
  • 12. Wash Buffer (PBS and PBS-T). Recipes:
    PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
    PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
  • *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0060.0730.1460.2740.5421.0641.8722.436

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)85.622751982.8236527
Standard deviation3.8512.730.16.1314.637.4
CV (%)4.5%5.6%5.8%7.4%6.2%7.1%

Gene/Protein Information For PPBP (Source: Uniprot.Org, NCBI)

Gene Name

PPBP

Full Name

Platelet basic protein

Weight

13.894kDa

Superfamily

intercrine alpha (chemokine CxC) family

Alternative Names

Beta-TG; connective tissue-activating peptide III; CTAP III; CTAP3CTAP-III; CTAPIII; CXC chemokine ligand 7; C-X-C motif chemokine 7; CXCL7; CXCL7b-TG1; LA-PF4; LDGFTC1; Leukocyte-derived growth factor; low-affinity platelet factor IV; Macrophage-derived growth factor; MDGFTC2; NAP2; NAP-2; NAP-2-L1; neutrophil-activating peptide 2; neutrophil-activating peptide-2; PBPTHBGB; platelet basic protein; PPBP; pro-platelet basic protein (chemokine (C-X-C motif) ligand 7); SCAR10; SCYB7beta-thromboglobulin; small inducible cytokine B7; small inducible cytokine subfamily B, member 7; Small-inducible c PPBP B-TG1, Beta-TG, CTAP-III, CTAP3, CTAPIII, CXCL7, LA-PF4, LDGF, MDGF, NAP-2, PBP, SCYB7, TC1, TC2, TGB, TGB1, THBGB, THBGB1 pro-platelet basic protein platelet basic protein|C-X-C motif chemokine 7|CXC chemokine ligand 7|beta-thromboglobulin|chemokine (C-X-C motif) ligand 7|connective tissue-activating peptide III|leukocyte-derived growth factor|low-affinity platelet factor IV|macrophage-derived growth factor|neutrophil-activating peptide 2|small inducible cytokine B7|small inducible cytokine subfamily B, member 7|thrombocidin 1|thrombocidin 2|thromboglobulin, beta-1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on PPBP, check out the PPBP Infographic

PPBP infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for PPBP: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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