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Product Info Summary
| SKU: | EK0575 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human EG-VEGF/PK1/PROK1 ELISA Kit PicoKine®
SKU/Catalog Number
EK0575
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
PROK1 (prokineticin-1; EG-VEGF/PK1) is a secreted factor with growth factor activity involved in endothelial proliferation/migration and angiogenesis/vascular fenestration (GO:0001525 angiogenesis). Assay context: pre-coated sandwich ELISA for Human PROK1 in culture supernatants, cell lysates, serum, and plasma; datasheet reports sensitivity <7 pg/mL and a 15.6–1,000 pg/mL detection range. Often interpreted alongside endothelial/vascular markers such as CD34, and can be combined into broader secretome panels using multiplex assays (putative).
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human EG-VEGF/PK1/PROK1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0575)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: A20-F105
Capture/Detection Antibodies
The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat.
Sensitivity
<7 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0575 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0575 is reactive to PROK1 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0575 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of PROK1
EG-VEGF, Endocrine glandderived vascular endothelial growth factor, is a human protein encoded by by the PROKR1 gene. Its gene is mapped to chromosome 1. The 1.4-kb cDNA encodes a protein of 105 amino acids with a well defined signal sequence. The mature protein is predicted to have 86 amino acids. Northern blot analysis demonstrated expression in testis, ovary, adrenal gland, and placenta. EG-VEGF has played a vital role in inducing proliferation, migration, and fenestration in capillary endothelial cells derived from endocrine glands. The standards of this kit are recombinant human EG-VEGF (A20-F105), with molecular weight of 10.5 KDa.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0575-CAP | Anti-Human PROK1 Pre-coated 96-well strip microplate | 1 |
| EK0575-ST | Human PROK1 Standard | 2 vials, 10 ng/tube |
| EK0575-DA | Human PROK1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.032 | 0.137 | 0.228 | 0.414 | 0.74 | 1.402 | 2.049 | 2.447 |
Data Example Images
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Human EG-VEGF PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect PROK1, Dilution ratio of 1:1, concentration in serum and plasma is around 100 pg/ml..
Some articles we found to cite concentrations of PROK1 in samples: 22847371 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human EG-VEGF/PK1/PROK1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 31 | 249 | 474 | 31 | 228 | 490 |
| Standard deviation | 1.45 | 18.17 | 30.81 | 1.61 | 18.92 | 39.2 |
| CV (%) | 4.7% | 7.3% | 6.5% | 5.2% | 8.3% | 8% |
Specific Publications For Human EG-VEGF/PK1/PROK1 ELISA Kit PicoKine® (EK0575)
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Human EG-VEGF/PK1/PROK1 ELISA Kit PicoKine®
Question
Q: how can I thaw whole blood sample for EG-VEGF ELISA after freezing?
Verified Customer
Verified customer
Asked: 2020-12-21
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test EG-VEGF for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2020-12-21
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
B. Hernandez
Verified customer
Asked: 2020-09-06
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-09-06
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
H. Thomas
Verified customer
Asked: 2020-07-27
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-07-27
Question
Q: is it okay to use citrate plasma as samples in Human EG-VEGF Picokine® ELISA Kit (Catalog # EK0575)?
B. Li
Verified customer
Asked: 2019-11-24
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of EG-VEGF causing disruption of its protein structure. EG-VEGF may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the EG-VEGF ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-11-24
Question
Q: What is the optimal O.D. value for EG-VEGF ELISA kit? I performed your EG-VEGF ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain EG-VEGF even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2018-07-10
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this EG-VEGF ELISA kit to have sensitivity of 7pg/ml, that means the minimum amount of EG-VEGF that can be declared/interpreted as positive by the above standard is 7pg/ml.
Boster Scientific Support
Answered: 2018-07-10
Question
Q: how to proceed with the analysis of ELISA data? I have obtained EG-VEGF level in serum.
Verified Customer
Verified customer
Asked: 2016-05-09
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/elisa-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/elisa-data-analysis-online
Boster Scientific Support
Answered: 2016-05-09
