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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
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Human EG-VEGF/PK1/PROK1 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human EG-VEGF / PK1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human PROK1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 7pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human EG-VEGF/PK1/PROK1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0575)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: A20-F105
15.6 pg/ml - 1,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0575 is reactive to PROK1 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
EK0575 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of EG-VEGF/PK1
EG-VEGF, Endocrine glandderived vascular endothelial growth factor, is a human protein encoded by by the PROKR1 gene. Its gene is mapped to chromosome 1. The 1.4-kb cDNA encodes a protein of 105 amino acids with a well defined signal sequence. The mature protein is predicted to have 86 amino acids. Northern blot analysis demonstrated expression in testis, ovary, adrenal gland, and placenta. EG-VEGF has played a vital role in inducing proliferation, migration, and fenestration in capillary endothelial cells derived from endocrine glands. The standards of this kit are recombinant human EG-VEGF (A20-F105), with molecular weight of 10.5 KDa.
|EK0575-CAP||Anti-Human PROK1 Pre-coated 96-well strip microplate||1|
|EK0575-ST||Human PROK1 Standard||2 vials, 10 ng/tube|
|EK0575-DA||Human PROK1 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human EG-VEGF PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect EG-VEGF/PK1, Dilution ratio of 1:1, concentration in serum is around 100pg/ml.
Some articles we found to cite concentrations of EG-VEGF/PK1 in samples: 22847371 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human EG-VEGF/PK1/PROK1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of EG-VEGF/PK1 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For PROK1 (Source: Uniprot.Org, NCBI)
AVIT (prokineticin) family
EGVEGF; EG-VEGF; EGVEGFblack mamba toxin-related protein; Endocrine-gland-derived vascular endothelial growth factor; Mambakine; PK1mambakine; PRK1prokineticin-1; PROK1; Prokineticin 1 PROK1 EGVEGF, PK1, PRK1 prokineticin 1 prokineticin-1|EG-VEGF|black mamba toxin-related protein|endocrine-gland-derived vascular endothelial growth factor|mambakine*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on PROK1, check out the PROK1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for PROK1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0575 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Knockout of αA-Crystallin Inhibits Ocular Neovascularization
Use of prokineticin-1 (PROK1), pregnancy-associated plasma protein A (PAPP-A) and PROK1/PAPP-A ratio to predict adverse pregnancy outcomes in the first trimester: a prospective study
Relationship between second-trimester amniotic fluid levels of Prokineticin-1 and Matrix Metalloproteinase-2 with adverse pregnancy outcome
Efficacy of EP chemotherapy followed by IP chemotherapy combined with radiotherapy in the treatment of extensive-stage small-cell lung cancer. Shiyang Kang,Chaopeng Ou,Ruifeng Xue,Weian Zeng,Jingxiu Huang,Yingjun Zhang,Dongtai Chen,Jielan Lai
EK0575 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Use of prokineticin-1 (PROK1), pregnancy-associated plasma protein A (PAPP-A) and PROK1/PAPP-A ratio to predict adverse pregnancy outcomes in the first trimester: a prospective study.
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