Human EG-VEGF/PK1/PROK1 ELISA Kit PicoKine™

Human EG-VEGF / PK1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human PROK1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 7pg/ml. Cited in 2 publication(s).

Product Info Summary

SKU: EK0575
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human EG-VEGF/PK1/PROK1 ELISA Kit PicoKine™

See all EG-VEGF/PK1 products

SKU/Catalog Number

EK0575

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human EG-VEGF / PK1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human PROK1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 7pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human EG-VEGF/PK1/PROK1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0575)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: A20-F105

Sensitivity

<7 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0575 is reactive to PROK1 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0575 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0575-CAP Anti-Human PROK1 Pre-coated 96-well strip microplate 1
EK0575-ST Human PROK1 Standard 2 vials, 10 ng/tube
EK0575-DA Human PROK1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.1230.1670.2030.2870.4680.7341.2462.050

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect EG-VEGF/PK1, Dilution ratio of 1:1, concentration in serum is around 100pg/ml.

Some articles we found to cite concentrations of EG-VEGF/PK1 in samples: 22847371 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human EG-VEGF/PK1/PROK1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3124947431228490
Standard deviation1.4518.1730.811.6118.9239.2
CV (%)4.7%7.3%6.5%5.2%8.3%8%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of EG-VEGF/PK1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 131353033321.926%
Sample 224923821622123113.25.7%
Sample 347440947446645527.185.9%
*number of samples for each test n=16.

Gene/Protein Information For PROK1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P58294

Gene ID

84432

Gene Name

PROK1

Full Name

Prokineticin-1

Weight

11.715kDa

Superfamily

AVIT (prokineticin) family

Alternative Names

EGVEGF; EG-VEGF; EGVEGFblack mamba toxin-related protein; Endocrine-gland-derived vascular endothelial growth factor; Mambakine; PK1mambakine; PRK1prokineticin-1; PROK1; Prokineticin 1 PROK1 EGVEGF, PK1, PRK1 prokineticin 1 prokineticin-1|EG-VEGF|black mamba toxin-related protein|endocrine-gland-derived vascular endothelial growth factor|mambakine

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on PROK1, check out the PROK1 Infographic

PROK1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for PROK1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0575 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Efficacy of EP chemotherapy followed by IP chemotherapy combined with radiotherapy in the treatment of extensive-stage small-cell lung cancer. Shiyang Kang,Chaopeng Ou,Ruifeng Xue,Weian Zeng,Jingxiu Huang,Yingjun Zhang,Dongtai Chen,Jielan Lai
Species: Human
EK0575 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Use of prokineticin-1 (PROK1), pregnancy-associated plasma protein A (PAPP-A) and PROK1/PAPP-A ratio to predict adverse pregnancy outcomes in the first trimester: a prospective study.

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6 Customer Q&As for Human EG-VEGF/PK1/PROK1 ELISA Kit PicoKine™

Question

Q: how can I thaw whole blood sample for EG-VEGF ELISA after freezing?

Verified Customer

Verified customer

Asked: 2020-12-21

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test EG-VEGF for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2020-12-21

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

B. Hernandez

Verified customer

Asked: 2020-09-06

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2020-09-06

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

H. Thomas

Verified customer

Asked: 2020-07-27

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2020-07-27

Question

Q: is it okay to use citrate plasma as samples in Human EG-VEGF Picokine® ELISA Kit (Catalog # EK0575)?

B. Li

Verified customer

Asked: 2019-11-24

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of EG-VEGF causing disruption of its protein structure. EG-VEGF may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the EG-VEGF ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2019-11-24

Question

Q: What is the optimal O.D. value for EG-VEGF ELISA kit? I performed your EG-VEGF ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain EG-VEGF even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2018-07-10

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this EG-VEGF ELISA kit to have sensitivity of 7pg/ml, that means the minimum amount of EG-VEGF that can be declared/interpreted as positive by the above standard is 7pg/ml.

Boster Scientific Support

Answered: 2018-07-10

Question

Q: how to proceed with the analysis of ELISA data? I have obtained EG-VEGF level in serum.

Verified Customer

Verified customer

Asked: 2016-05-09

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2016-05-09

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