Click image to see more details
Product Info Summary
| SKU: | EK0575 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human EG-VEGF/PK1/PROK1 ELISA Kit PicoKine®
View all EG-VEGF/PK1 ELISA kits
SKU/Catalog Number
EK0575
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human EG-VEGF / PK1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human PROK1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 7pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human EG-VEGF/PK1/PROK1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0575)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: A20-F105
Sensitivity
<7 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0575 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0575 is reactive to PROK1 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0575 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of EG-VEGF/PK1
EG-VEGF, Endocrine glandderived vascular endothelial growth factor, is a human protein encoded by by the PROKR1 gene. Its gene is mapped to chromosome 1. The 1.4-kb cDNA encodes a protein of 105 amino acids with a well defined signal sequence. The mature protein is predicted to have 86 amino acids. Northern blot analysis demonstrated expression in testis, ovary, adrenal gland, and placenta. EG-VEGF has played a vital role in inducing proliferation, migration, and fenestration in capillary endothelial cells derived from endocrine glands. The standards of this kit are recombinant human EG-VEGF (A20-F105), with molecular weight of 10.5 KDa.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0575-CAP | Anti-Human PROK1 Pre-coated 96-well strip microplate | 1 |
| EK0575-ST | Human PROK1 Standard | 2 vials, 10 ng/tube |
| EK0575-DA | Human PROK1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
Submit A Review
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.123 | 0.167 | 0.203 | 0.287 | 0.468 | 0.734 | 1.246 | 2.050 |
Data Example Images
Click image to see more details
Human EG-VEGF PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect EG-VEGF/PK1, Dilution ratio of 1:1, concentration in serum and plasma is around 100 pg/ml..
Some articles we found to cite concentrations of EG-VEGF/PK1 in samples: 22847371 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human EG-VEGF/PK1/PROK1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 31 | 249 | 474 | 31 | 228 | 490 |
| Standard deviation | 1.45 | 18.17 | 30.81 | 1.61 | 18.92 | 39.2 |
| CV (%) | 4.7% | 7.3% | 6.5% | 5.2% | 8.3% | 8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of EG-VEGF/PK1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 31 | 35 | 30 | 33 | 32 | 1.92 | 6% |
| Sample 2 | 249 | 238 | 216 | 221 | 231 | 13.2 | 5.7% |
| Sample 3 | 474 | 409 | 474 | 466 | 455 | 27.18 | 5.9% |
Protein Target Info & Infographic
Gene/Protein Information For PROK1 (Source: Uniprot.Org, NCBI)
Gene Name
PROK1
Full Name
Prokineticin-1
Weight
11.715kDa
Superfamily
AVIT (prokineticin) family
Alternative Names
EGVEGF; EG-VEGF; EGVEGFblack mamba toxin-related protein; Endocrine-gland-derived vascular endothelial growth factor; Mambakine; PK1mambakine; PRK1prokineticin-1; PROK1; Prokineticin 1 PROK1 EGVEGF, PK1, PRK1 prokineticin 1 prokineticin-1|EG-VEGF|black mamba toxin-related protein|endocrine-gland-derived vascular endothelial growth factor|mambakine
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on PROK1, check out the PROK1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for PROK1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human EG-VEGF/PK1/PROK1 ELISA Kit PicoKine® (EK0575)
Hello CJ!
EK0575 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Knockout of αA-Crystallin Inhibits Ocular Neovascularization
Use of prokineticin-1 (PROK1), pregnancy-associated plasma protein A (PAPP-A) and PROK1/PAPP-A ratio to predict adverse pregnancy outcomes in the first trimester: a prospective study
Relationship between second-trimester amniotic fluid levels of Prokineticin-1 and Matrix Metalloproteinase-2 with adverse pregnancy outcome
Efficacy of EP chemotherapy followed by IP chemotherapy combined with radiotherapy in the treatment of extensive-stage small-cell lung cancer. Shiyang Kang,Chaopeng Ou,Ruifeng Xue,Weian Zeng,Jingxiu Huang,Yingjun Zhang,Dongtai Chen,Jielan Lai
Species: Human
EK0575 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Use of prokineticin-1 (PROK1), pregnancy-associated plasma protein A (PAPP-A) and PROK1/PAPP-A ratio to predict adverse pregnancy outcomes in the first trimester: a prospective study.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Human EG-VEGF/PK1/PROK1 ELISA Kit PicoKine®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Human EG-VEGF/PK1/PROK1 ELISA Kit PicoKine®
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
6 Customer Q&As for Human EG-VEGF/PK1/PROK1 ELISA Kit PicoKine®
Question
Q: how can I thaw whole blood sample for EG-VEGF ELISA after freezing?
Verified Customer
Verified customer
Asked: 2020-12-21
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test EG-VEGF for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2020-12-21
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
B. Hernandez
Verified customer
Asked: 2020-09-06
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-09-06
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
H. Thomas
Verified customer
Asked: 2020-07-27
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-07-27
Question
Q: is it okay to use citrate plasma as samples in Human EG-VEGF Picokine® ELISA Kit (Catalog # EK0575)?
B. Li
Verified customer
Asked: 2019-11-24
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of EG-VEGF causing disruption of its protein structure. EG-VEGF may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the EG-VEGF ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-11-24
Question
Q: What is the optimal O.D. value for EG-VEGF ELISA kit? I performed your EG-VEGF ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain EG-VEGF even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2018-07-10
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this EG-VEGF ELISA kit to have sensitivity of 7pg/ml, that means the minimum amount of EG-VEGF that can be declared/interpreted as positive by the above standard is 7pg/ml.
Boster Scientific Support
Answered: 2018-07-10
Question
Q: how to proceed with the analysis of ELISA data? I have obtained EG-VEGF level in serum.
Verified Customer
Verified customer
Asked: 2016-05-09
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2016-05-09
