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Product Info Summary
| SKU: | EK0799 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human IL-27 ELISA Kit PicoKine®
SKU/Catalog Number
EK0799
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human IL-27 ELISA Kit PicoKine™ (96 Tests). Quantitate Human IL27 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human IL-27 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0799)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: EBI3 (R21-K229) + P28 (F29-P243)
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0799 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0799 is reactive to IL27 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0799 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IL27
Interleukin-27 (IL-27) is a heterodimeric cytokine belonging to the IL-12 family that is composed of two subunits; Epstein-Barr virus (EBV)-induced gene 3 (EBI3) (also known as IL-27B) and IL27-p28 (known as IL-30). IL-27 is produced by antigen-presenting cells.IL-27 plays an important function in regulating the activity of B- and T-lymphocytes. IL-27, a potent inhibitor of T (H)-17 cell development, may be a useful target for treating inflammatory diseases mediated by these cells.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0799-CAP | Anti-Human IL27 Pre-coated 96-well strip microplate | 1 |
| EK0799-ST | Human IL27 Standard | 2 vials, 10 ng/tube |
| EK0799-DA | Human IL27 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
Submit A Review
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.075 | 0.132 | 0.184 | 0.277 | 0.529 | 1.006 | 1.497 | 1.905 |
Data Example Images
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Human IL-27 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect IL27, Dilution ratio of 1:1, concentration in serum and plasma is 0-400 pg/ml..
Some articles we found to cite concentrations of IL27 in samples: 23645091 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human IL-27 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 266 | 1556 | 5164 | 259 | 1491 | 5174 |
| Standard deviation | 14.63 | 716.05 | 256.2% | 16.05 | 90.95 | 284.57 |
| CV (%) | 5.5% | 5% | 5% | 6.2% | 6.1% | 5.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of IL27 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 266 | 272 | 277 | 241 | 264 | 13.83 | 5.2% |
| Sample 2 | 1556 | 1617 | 1541 | 1521 | 1558 | 35.84 | 2.3% |
| Sample 3 | 5164 | 5696 | 5356 | 5370 | 5396 | 191.11 | 3.5% |
Protein Target Info & Infographic
Gene/Protein Information For IL27 (Source: Uniprot.Org, NCBI)
Gene Name
IL27
Full Name
Interleukin-27 subunit alpha
Weight
27.493kDa
Superfamily
IL-6 superfamily
Alternative Names
IL-27 p28 subunit; IL-27 subunit alpha; IL27; IL-27; IL27A; IL-27-A; IL27-A; IL27p28; IL30; interleukin 27; interleukin 30; interleukin-27 subunit alpha; MGC71873; p28IL-27A IL27 IL-27, IL-27AA, IL27p28, IL30, p28, IL27 interleukin 27 interleukin-27 subunit alpha|IL-27 p28 subunit|IL-27 subunit alpha|IL-27-A|IL27-A|interleukin-30
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on IL27, check out the IL27 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IL27: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human IL-27 ELISA Kit PicoKine® (EK0799)
Hello CJ!
EK0799 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effect of interferon-? on NF-?B and cytokine IL-18 and IL-27 in acute pancreatitis
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Customer Reviews
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*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
9 Customer Q&As for Human IL-27 ELISA Kit PicoKine®
Question
Does EK0799 recognize only the p28 subunit or will it recognize the entire heterodimeric IL27 (p28+EBI3)?
Verified customer
Asked: 2019-10-30
Answer
Yes, the Human IL-27 ELISA Kit PicoKine™ (EK0799) can recognize both heterodimeric IL-27 and IL27(P28).
Boster Scientific Support
Answered: 2019-10-30
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2019-06-12
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-06-12
Question
Q: Can IL27 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
P. Sunder
Verified customer
Asked: 2018-09-02
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-09-02
Question
Q: if the enzyme conjugated IL27 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL27 antigen?
S. Moore
Verified customer
Asked: 2018-01-20
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-01-20
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2017-05-12
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2017-05-12
Question
I would like to ask if the immunogen was to generate the coating and detection antibodies for this Human IL-27 ELISA Kit PicoKine. Does it recognizes the p28/EBI3 heterodimer alone or does it pick up free p28 and EBI3 not in the heterodimer.
Verified Customer
Verified customer
Asked: 2017-02-09
Answer
The Human IL-27 ELISA Kit PicoKine can either recognizes free p28 or p28 bind to EBI3. We know that EBl3 do not recognize alone and p28 alone has not been assayed.
Boster Scientific Support
Answered: 2017-02-09
Question
Does the EK0799 kit recognize the heterodimeric IL27 (i.e. IL27p28 + EBI3) or only the Il27p28 subunit?
Verified Customer
Verified customer
Asked: 2016-10-25
Answer
The EK0799 kit can recognize both heterodimeric IL-27 and IL27(P28).
Boster Scientific Support
Answered: 2016-10-25
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2016-05-11
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2016-05-11
Question
Q: how to analyze ELISA data? I have obtained IL27 level in cell lysates.
E. Hall
Verified customer
Asked: 2014-03-26
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2014-03-26
