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Product Info Summary
| SKU: | EK0985 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human Periostin/OSF2 ELISA Kit PicoKine®
View all Periostin/OSF-2 ELISA kits
SKU/Catalog Number
EK0985
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Periostin/OSF2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human POSTN in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Periostin/OSF2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0985)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: N22-Q836
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0985 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0985 is reactive to POSTN in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0985 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Periostin/OSF-2
Periostin, also known as OSF2, is a protein that in humans is encoded by the POSTN gene. The International Radiation Hybrid Mapping Consortium mapped the POSTN gene to chromosome 13. Periostin functions as a ligand for alpha-V/beta-3 and alpha-V/beta-5 integrins to support adhesion and migration of epithelial cells. It is found that periostin was overexpressed by the majority of human primary breast cancers examined. After myocardial infarction, periostin-induced cardiomyocyte cell cycle reentry and mitosis were associated with improved ventricular remodeling and myocardial function, reduced fibrosis and infarct size, and increase angiogenesis.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0985-CAP | Anti-Human POSTN Pre-coated 96-well strip microplate | 1 |
| EK0985-ST | Human POSTN Standard | 2 vials, 10 ng/tube |
| EK0985-DA | Human POSTN Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.010 | 0.052 | 0.100 | 0.166 | 0.302 | 0.582 | 0.978 | 1.804 |
Data Example Images
Click image to see more details
Human Periostin/OSF2 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Periostin/OSF-2, Dilution ratio of 1:50, concentration in serum and plasma is around 60 ng/ml..
Some articles we found to cite concentrations of Periostin/OSF-2 in samples: 19212687 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Periostin/OSF2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 188 | 1707 | 4824 | 180 | 1664 | 4867 |
| Standard deviation | 8.64 | 124.61 | 192.96 | 11.34 | 136.44 | 199.54 |
| CV (%) | 4.6% | 7.3% | 4% | 8.2% | 8.2% | 4.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Periostin/OSF-2 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 188 | 183 | 213 | 194 | 194 | 11.36 | 5.8% |
| Sample 2 | 1707 | 1857 | 1800 | 1793 | 1789 | 53.58 | 2.9% |
| Sample 3 | 4824 | 4787 | 4733 | 4555 | 4724 | 103.2 | 2.1% |
Protein Target Info & Infographic
Gene/Protein Information For POSTN (Source: Uniprot.Org, NCBI)
Gene Name
POSTN
Full Name
Periostin
Weight
93.314kDa
Alternative Names
Fasciclin I-like; MGC119510; MGC119511; OSF2; OSF-2; OSF-2osteoblast specific factor 2 (fasciclin I-like); OSF2periodontal ligament-specific periostin; Osteoblast-specific factor 2; PDLPOSTN; periostin isoform thy2; periostin isoform thy4; periostin isoform thy6; periostin isoform thy8; Periostin; periostin, osteoblast specific factor; PNRP11-412K4.1; POSTN; TRIF52 POSTN OSF-2, OSF2, PDLPOSTN, PN periostin periostin|osteoblast specific factor 2 (fasciclin I-like)|periodontal ligament-specific periostin|periostin, osteoblast specific factor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on POSTN, check out the POSTN Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for POSTN: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Periostin/OSF2 ELISA Kit PicoKine® (EK0985)
Hello CJ!
EK0985 has been cited in 6 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Circulating Levels of IL-13, TGF-β1, and Periostin as Potential Biomarker for Coronary Artery Disease with Acute Heart Failure
TGFβ3-mediated induction of Periostin facilitates head and neck cancer growth and is associated with metastasis
Dysregulation of astrocyte extracellular signaling in Costello syndrome
Species: Human
TGF?3-mediated induction of Periostin facilitates head and neck cancer growth and is associated with metastasis
Li Y, Xie K, Chen H, Wang G, Yu Y. J Surg Res. 2015 Jun 1;196(1):136-48. Doi: 10.1016/J.Jss.2015.02.042. Epub 2015 Feb 27. Hydrogen Gas Inhibits High-Mobility Group Box 1 Release In Septic Mice By Upregulation Of Heme Oxygenase 1.
Krencik R, Hokanson Kc, Narayan Ar, Dvornik J, Rooney Ge, Rauen Ka, Weiss La, Rowitch Dh, Ullian Em. Sci Transl Med. 2015 May 6;7(286):286Ra66. Doi: 10.1126/Scitranslmed.Aaa5645. Dysregulation Of Astrocyte Extracellular Signaling In Costello Syndr...
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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14 Customer Q&As for Human Periostin/OSF2 ELISA Kit PicoKine®
Question
The datasheet says the immunogen sequence is N22-Q836. This kit has two antibodies, coated on the plate, and biotinylated antibody. Is the immunogen sequence used to generate the BOTH antibodies? Or, does each antibody detect different position of the target?
Verified customer
Asked: 2022-07-19
Answer
Thank you for your questions. Below are the answers. This kit has two antibodies, coated on the plate, and biotinylated antibody? ---Yes. Is the immunogen sequence used to generate the BOTH antibodies? Or, does each antibody detect different position of the target? ---The immunogen sequence used for the BOTH antibody is aa 1-836 (Uniprot#Q15063).
Boster Scientific Support
Answered: 2022-07-19
Question
I have some questions regarding EK0985, Human Periostin/OSF2 ELISA Kit PicoKine®. According to Uniprot website below regarding the target Periostin, there are 7 isoforms are known. https://www.uniprot.org/uniprotkb/Q15063/entry 1.Is it possible that the antibody pair detect all isoforms? 2.Do you mean that each antibody is raised against aa 1-836 of isoform1? 3.Have you tested detailed specificity using recombinant protein of the isoforms? 4. Could you provide me with the immunogen sequence used for the antibody pair in this kit?
Verified customer
Asked: 2022-07-18
Answer
Thank you for your questions. 1.Is it possible that the antibody pair detect all isoforms? It is possible that the antibody pair detects all isoforms. 2.Do you mean that each antibody is raised against aa 1-836 of isoform1? Yes, each antibody is raised against aa 1-836 of isoform1. 3.Have you tested detailed specificity using recombinant protein of the isoforms? No, we haven't. 4. Could you provide me with the immunogen sequence used for the antibody pair in this kit? The immunogen sequence used for the antibody pair is aa 1-836 (Uniprot#Q15063).
Boster Scientific Support
Answered: 2022-07-18
Question
We are working on monomeric Periostin in human samples, do you know if EK0985 kit detects the monomer only?
Verified customer
Asked: 2019-09-30
Answer
The Human Periostin/OSF2 ELISA Kit PicoKine EK0985 detects all confirmations of Periostin (monomer, homodimer, etc) nonspecifically. Usually, if our kits does not specify what it detects, then it means that it would detect all confirmations or isoforms of a target.
Boster Scientific Support
Answered: 2019-10-01
Question
Q: can you suggest the dilution ratio of serum samples for detection of Periostin in Human plasma? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2019-08-07
Answer
A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Periostin ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-08-07
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2019-05-05
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-05-05
Question
Q: Can Periostin ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
J. Dayal
Verified customer
Asked: 2019-03-04
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-03-04
Question
Q: which procedure should I follow in order to thaw whole blood sample for Periostin ELISA after freezing?
D. Wang
Verified customer
Asked: 2018-12-28
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Periostin for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2018-12-28
Question
Q: can I use citrate plasma as samples in Human Periostin Picokine® ELISA Kit (Catalog # EK0985)?
B. Kaur
Verified customer
Asked: 2018-11-10
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of Periostin causing degradation of its protein structure. Periostin may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the Periostin ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-11-10
Question
If the immunogen is UniProt Q15063, which isoform was used as the immunogen?
Verified Customer
Verified customer
Asked: 2018-07-02
Answer
Isoform 1 was used as the immunogen for EK0985.
Boster Scientific Support
Answered: 2018-07-02
Question
Q: how to analyze ELISA data? I have obtained Periostin level in plasma.
H. Li
Verified customer
Asked: 2018-06-04
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-06-04
Question
Q: if the enzyme conjugated Periostin antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Periostin antigen?
Verified Customer
Verified customer
Asked: 2018-03-03
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-03-03
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2017-09-22
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2017-09-22
Question
Are the capture and the detection antibodies immunized against the full-length protein? <br> Keywords: capture antibody, detection antibody, full length, partial, immunogen
Verified Customer
Verified customer
Asked: 2017-08-31
Answer
The capture and the detection antibodies are immunized against the full-length protein for EK0985.
Boster Scientific Support
Answered: 2017-08-31
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
W. Singh
Verified customer
Asked: 2015-09-20
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2015-09-20
