Human PROC/Protein C ELISA Kit PicoKine®

Product Name

Human PROC/Protein C ELISA Kit PicoKine®

View all Coagulation Factor XIV/Protein C ELISA kits

SKU/Catalog Number

EK1975

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human PROC/Protein C ELISA Kit PicoKine™ (96 Tests). Quantitate Human Proc in cell culture supernatants, serum and plasma (heparin, EDTA) and urine. Sensitivity: 0.1ng/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human PROC/Protein C ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1975)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: A43-P461

Sensitivity

<0.1 ng/ml

Assay Range

0.78 ng/ml - 50 ng/ml

Standard Dilution Instructions

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1975 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1975 is reactive to PROC in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA) and urine.

Application Guarantee

EK1975 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Coagulation Factor XIV/Protein C

Protein C (PROC), also called PC, is a zymogenic (inactive) protein, the activated form of which plays an important role in regulating blood clotting, inflammation, cell death and maintaining the permeability of blood vessel walls in humans and other animals. The PROC gene is mapped to 2q14.3. This gene encodes a vitamin K-dependent plasma glycoprotein. The encoded protein is cleaved to its activated form by the thrombinthrombomodulin complex. This activated form contains a serine protease domain and functions in degradation of the activated forms of coagulation factors V and VIII. Mutations in this gene have been associated with thrombophilia due to protein C deficiency, neonatal purpura fulminans, and recurrent venous thrombosis.

Kit Components

Catalog Number	Description	Quantity
EK1975-CAP	Anti-Human PROC Pre-coated 96-well strip microplate	1
EK1975-ST	Human PROC Standard	2 vials, 50 ng/tube
EK1975-DA	Human PROC Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop Solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Incubator.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (ng/ml)	0	0.78	1.56	3.12	6.25	12.5	25	50
O.D.	0.017	0.135	0.233	0.445	0.857	1.343	1.813	2.017

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Coagulation Factor XIV/Protein C, Dilution ratio of 1:200, concentration in serum and plasma is around 2.5 ug/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human PROC/Protein C ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Coagulation Factor XIV/Protein C in ELISA kits from four different production batches/lots.

Gene/Protein Information For PROC (Source: Uniprot.Org, NCBI)

Alternative Names

Anticoagulant protein C; APC; Autoprothrombin IIA; Blood coagulation factor XIV; Coagulation Factor XIV; EC 3.4.21; EC 3.4.21.69; PC; PROC; PROC1; protein C (inactivator of coagulation factors Va and VIIIa); Protein C; vitamin K-dependent protein C PROC APC, PC1, THPH3, THPH4, PROC protein C, inactivator of coagulation factors Va and VIIIa vitamin K-dependent protein C|Protein C-Nagoya|activated protein C|anticoagulant protein C|autoprothrombin IIA|blood coagulation factor XIV|prepro-protein C|type I protein C

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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