Human SPINT1/HAI-1 PicoKine™ Quick ELISA Kit

Human SPINT1/HAI-1 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human SPINT1 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 15pg/ml.

Product Info Summary

SKU: FEK0771
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin)
Assay Time: 90 Minutes

Product Name

Human SPINT1/HAI-1 PicoKine™ Quick ELISA Kit

See all HAI-1/HGFA Inhibitor 1 products

SKU/Catalog Number

FEK0771

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human SPINT1/HAI-1 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human SPINT1 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 15pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human SPINT1/HAI-1 PicoKine™ Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0771)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: G36-L529

Sensitivity

<15 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK0771 is reactive to SPINT1 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin)

Application Guarantee

FEK0771 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog numberDescription QuantityVolume
FEK0771-CAPAnti-tag Pre-coated 96-well Strip Microplate 112 strips of 8 wells
FEK0771-STHuman HAI-1/HGFA Inhibitor 1 Standard 2 10 ng/tube
FEK0771-CHuman SPINT1 Antibody Cocktail 1 6ml
AR1106-1Sample Diluent 1 15ml
AR1106-7TBS-T Wash Buffer (25x) 1 12ml
AR1104Color Developing Reagent (TMB) 1 10ml
AR1105Stop Solution 1 10ml
PLA-SEAAdhesive Plate Sealers 2 Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional)
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.
  • Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0360.1020.1500.2430.4210.7651.3832.228

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2111855388723418523784
Standard deviation13.5116.87264.3217.55120.38298.94
CV (%)6.4%6.3%6.8%7.5%6.5%7.9%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of HAI-1/HGFA Inhibitor 1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 121124420823322412.325.5%
Sample 21855167516941706173339.852.3%
Sample 338874423426346994318250.445.8%
*number of samples for each test n=16.

Gene/Protein Information For SPINT1 (Source: Uniprot.Org, NCBI)

Uniprot ID

O43278

Gene ID

6692

Gene Name

SPINT1

Full Name

Kunitz-type protease inhibitor 1

Weight

58.398kDa

Alternative Names

HAI; HAI1; HAI-1; hepatocyte growth factor activator inhibitor 1; Hepatocyte growth factor activator inhibitor type 1; kunitz-type protease inhibitor 1; MANSC2; serine peptidase inhibitor, Kunitz type 1; serine protease inhibitor, Kunitz type 1; SPINT1 SPINT1 HAI, HAI1, MANSC2 serine peptidase inhibitor, Kunitz type 1 kunitz-type protease inhibitor 1|HAI-1|hepatocyte growth factor activator inhibitor type 1|serine protease inhibitor, Kunitz type 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on SPINT1, check out the SPINT1 Infographic

SPINT1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for SPINT1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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