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CD34 antibody (PA1334) performs well in Flow Cytometry, IHC, IHC-F, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 72 Publications

pa1334 cd34 primary antibodies fcm testing 10

Flow Cytometry analysis of HEL cells using anti-CD34 antibody (PA1334).
Overlay histogram showing HEL cells stained with PA1334 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD34 Antibody (PA1334, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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Anti-CD34 Antibody Picoband®

CD34 antibody

72 Publications

Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	Flow Cytometry, IHC, IHC-F, WB

Tested Applications

CD34 antibody was positively detected by WB in:

human placenta tissue, rat brain tissue, mouse brain tissue

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

CD34 antibody was positively detected by IHC in:

Rat Kidney tissue, Human Lung Cancer tissue, human liver cancer tissue, human placenta tissue, mouse kidney tissue, rat kidney tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

CD34 antibody was positively detected by IHC-F in:

Rat Placenta tissue, Rat Kidney tissue

CD34 antibody was positively detected by FC in:

HEL cell

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Human

Tested

Recommend

Rat

Tested

Recommend

Mouse

Tested

Recommend

Rabbit

Predicted

Monkey

Predicted

Bovine

Predicted

Sheep

Predicted

Dog

Predicted

Product Information

Product Name

Anti-CD34 Antibody Picoband®
View all CD34 Antibodies

Form

Lyophilized

Uniprot

P28906

Gene ID

947

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.01mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human CD34, identical to the related mouse and rat sequences.

Cross-Reactivity

No cross-reactivity with other proteins

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

105 kDa

Calculated Molecular Weight

40.7 kDa

Background of CD34

CD34 is a monomeric cell surface antigen with a molecular mass of approximately 110 KD.CD34 is expressed in humans in hematopoietic stem cells, vascular endothelium, and blasts from 30% of patients with acute myeloid and lymphocytic leukemia. The human CD34 gene spans 26 kb and has 8 exons, a structure quite similar to that of the murine gene. By Southern blot analysis of DNA from a panel of human x mouse somatic cell hybrids using a CD34 cDNA probe demonstrate that the gene for CD34 is located on human chromosome 1 in the 1q12----qter region. CD34 plays an important role in the formation of progenitor cells during both embryonic and adult hematopoiesis.

Cite This Product

Anti-CD34 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1334)

Protocols

Western Blot protocol for CD34 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for CD34 antibody

Review Protocol

Flow Cytometry (FC) protocol for CD34 antibody

Review Protocol

Publications

DPPA inhibits melanoma by targeting angiogenesis through activating autocrine IFN-γ-CXCL9/10/11-CXCR3 axis in vascular endothelial cells

Published: 2026-06-10

Journal: iScience

Application: IHC

Impact Factor: 4.50

Species: Mouse

PMID: 42305612

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Free Secondary Antibody

Anti-CD34 Antibody Picoband®

CD34 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

DPPA inhibits melanoma by targeting angiogenesis through activating autocrine IFN-γ-CXCL9/10/11-CXCR3 axis in vascular endothelial cells

Echinacoside improves antioxidant responses and angiogenesis through Parkin-MFN2-mediated mitophagy to promote diabetic wound healing

Kynurenine promotes angiogenesis through mTOR signaling in head and neck squamous cell carcinoma

D-chiro-inositol effectively counteracts endometriosis in a mouse model

A pilot study examining periodontally healthy middle-aged humans and monkeys display different levels of alveolar bone resorption, gingival inflammatory infiltrate, and salivary microbiota profile

Vascularized characteristics and functional regeneration of three-dimensional cell reconstruction of oral mucosa equivalents based on vascular homeostasis phenotypic modification

Hematopoietic Function Restoration by Transplanting Bone Marrow Niches In Vivo Engineered Using Carbonate Apatite Honeycomb Bioreactors

Reversibly immortalization establishes a hair follicle stem cell line with hair follicle reconstruction ability

Gualou Guizhi decoction promotes therapeutic angiogenesis via the miR210/HIF/VEGF pathway in vivo and in vitro

OLFML3, as a potential predictor of prognosis and therapeutic target for glioma, is closely related to immune cell infiltration

Sinomenine promotes flap survival by upregulating eNOS and eNOS-mediated autophagy via PI3K/AKT pathway

Steppogenin suppresses tumor growth and sprouting angiogenesis through inhibition of HIF-1α in tumors and DLL4 activity in the endothelium

A mouse model of irradiation and spleen-thymus lymphocyte infusion induced aplastic anemia

Histology of tendon and enthesis - suitable techniques for specific research questions

Novel dopamine-modified oxidized sodium alginate hydrogels promote angiogenesis and accelerate healing of chronic diabetic wounds

E7-Targeted Nanotherapeutics for Key HPV Afflicted Cervical Lesions by Employing CRISPR/Cas9 and Poly (Beta-Amino Ester)

Multiple Intravenous Injections of Valproic Acid-Induced Mesenchymal Stem Cell from Human-Induced Pluripotent Stem Cells Improved Cardiac Function in an Acute Myocardial Infarction Rat Model

Knocking out matrix metalloproteinase 12 causes the accumulation of M2 macrophages in intestinal tumor microenvironment of mice

Asiaticoside nitric oxide gel accelerates diabetic cutaneous ulcers healing by activating Wnt/β-catenin signaling pathway

NO donor inhibits proliferation and induces apoptosis by targeting PI3K/AKT/mTOR and MEK/ERK pathways in hepatocellular carcinoma cells

Telocytes as a Novel Structural Component in the Muscle Layers of the Goat Rumen

SIRT1 participates in the response to methylglyoxal-dependent glycative stress in mouse oocytes and ovary

The protective role of intermedin in promoting angiogenesis during renal fibrosis

Small bowel gastrointestinal stromal tumor: a retrospective study of 32 cases at a single center and review of the literature

Nanoparticles Based on Poly (β-Amino Ester) and HPV16-Targeting CRISPR/shRNA as Potential Drugs for HPV16-Related Cervical Malignancy

Human nail stem cells are retained but hypofunctional during aging

Synthetic E-selectin prevents postoperative vascular restenosis by inhibiting nuclear factor κB in rats

Experimental evidence of good efficacy and reduced toxicity with peptide-doxorubicin to treat gastric cancer

MicroRNA-34a induces transdifferentiation of glioma stem cells into vascular endothelial cells by targeting Notch pathway

Metastasis-Associated Protein 1 Deficiency Results in Compromised Pulmonary Alveolar Capillary Angiogenesis in Mice

The role of 5-HT2B receptors in mitral valvulopathy: bone marrow mobilization of endothelial progenitors

Clinical biocharacterization of immunophenotype in hepatocellular carcinoma patients

In vivo inhibitory activity of andrographolide derivative ADN-9 against liver cancer and its mechanisms involved in inhibition of tumor angiogenesis

Efficacy of recombinant human adenovirus-p53 combined with chemotherapy for locally advanced cervical cancer: A clinical trial

Dipalmitoylphosphatidic acid inhibits tumor growth in triple-negative breast cancer

CD47 deficiency in tumor stroma promotes tumor progression by enhancing angiogenesis

Treatment of diabetes mellitus-induced erectile dysfunction using endothelial progenitor cells genetically modified with human telomerase reverse transcriptase

Incomplete radiofrequency ablation accelerates proliferation and angiogenesis of residual lung carcinomas via HSP70/HIF-1α

Platelet-rich plasma reduces skin flap inflammatory cells infiltration and improves survival rates through induction of angiogenesis: An experiment in rabbits

Secreted recombinant human IL-24 protein inhibits the proliferation of esophageal squamous cell carcinoma Eca-109 cells in vitro and in vivo

Assessment of Vascularity in Hepatic Alveolar Echinococcosis: Comparison of Quantified Dual-Energy CT with Histopathologic Parameters

Contrast-Enhanced Ultrasonography of Pancreatic Carcinoma: Correlation with Pathologic Findings

Canonical and non-canonical Wnt signaling control the regeneration of amputated rodent vibrissae follicles

Effect of Mini-Tyrosyl-tRNA Synthetase/Mini-Tryptophanyl-tRNA Synthetase on Angiogenesis in Rhesus Monkeys after Acute Myocardial Infarction

Overexpression of monocarboxylate anion transporter 1 and 4 in T24-induced cancer-associated fibroblasts regulates the progression of bladder cancer cells in a 3D microfluidic device

Expression of Wnt/β-catenin signaling, stem-cell markers and proliferating cell markers in rat whisker hair follicles

Ultrastructure damage of oviduct telocytes in rat model of acute salpingitis

Ephrin-B2 mediates trophoblast-dependent maternal spiral artery remodeling in first trimester

SOCS3 blocks HIF-1α expression to inhibit proliferation and angiogenesis of human small cell lung cancer by downregulating activation of Akt, but not STAT3

Telocytes damage in endometriosis-affected rat oviduct and potential impact on fertility

Different Effects of Implanting Sensory Nerve or Blood Vessel on the Vascularization, Neurotization, and Osteogenesis of Tissue-Engineered Bone In Vivo

Epidermal Growth Factor-Like Domain-Containing Protein 7 (EGFL7) Enhances EGF Receptor−AKT Signaling, Epithelial−Mesenchymal Transition, and Metastasis of Gastric Cancer Cells

Andrographolide Suppress Tumor Growth by Inhibiting TLR4/NF-κB Signaling Activation in Insulinoma

Distinct molecular basis for endothelial differentiation: Gene expression profiles of human mesenchymal stem cells versus umbilical vein endothelial cells

Immobilization of heparin/poly-l-lysine nanoparticles on dopamine-coated surface to create a heparin density gradient for selective direction of platelet and vascular cells behavior

Correlation between TSP-1, TGF-β and PPAR-γ expression levels and glioma microvascular density

Construction of Polyfunctional Coatings Assisted by Gallic Acid to Facilitate Co-Immobilization of Diverse Biomolecules

Targeting therapy of hepatocellular carcinoma with doxorubicin prodrug PDOX increases anti-metastatic effect and reduces toxicity: a preclinical study

Expression of Rac1, HIF-1α, and VEGF in Gastric Carcinoma: Correlation with Angiogenesis and Prognosis

Necrosis Degree Displayed in Computed Tomography Images Correlated With Hypoxia and Angiogenesis in Breast Cancer

Histopathological studies of microtubule disassembling agent-induced myocardial lesions in rats

Inhibition of Intestinal Adenoma Formation in APCMin/+ Mice by Riccardin D, a Natural Product Derived from Liverwort Plant Dumortiera hirsuta

CIP-13F, a novel aminopeptidase N (APN/CD13) inhibitor, inhibits Lewis lung carcinoma growth and metastasis in mice

Inhibition of angiogenesis involves in anticancer activity of riccardin D, a macrocyclic bisbibenzyl, in human lung carcinoma

LYP, a bestatin dimethylaminoethyl ester, inhibited cancer angiogenesis both in vitro and in vivo

Role of axonal guidance factor netrin-1 in human placental vascular growth

Effect of mini-tyrosyl-tRNA synthetase/mini-tryptophanyl-tRNA synthetase on ischemic angiogenesis in rats: proliferation and migration of endothelial cells