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Boster offers custom polyclonal antibody production for researchers who use non-mammalian models such as Zebrafish, Drophila, C. elegans and Yeast at $600. Contact us for a free consultation.
Boster offers high quality custom antibody production services, including Rabbit and Mouse monoclonal, as well as rabbit polyclonal. For Hu, Mo and Ra targets, we provide Immunoassay development service.For non-hu-mo-ra targets,take advantage of our $600 rare species custom polyclonal program.
Boster provides plate-based multiplex cytokine immunoassay service for analytes from human, mouse and rat. Contact us today and get a free consultation.
Boster offers custom recombinant protein expression service. Available expresssion systems include E. Coli, Yeast, Insect and Mamalian Cells. Get a free consultation today.
Boster offers custom DNA synthesis service for as low as $0.08 per bp. Any gene cloning into any vector, 100% accuracy, Fast turn around time. Get a free consultation today.
Boster offers a full range of lab services for immunohistochemistry (IHC) and immunofluorescence (IF). Get a free consultation today.
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The following reagents are used to generate the images below.
Anti-CD34 antibody, PA1334, Western blottingLane 1: Recombinant Human CD34 Protein 10ngLane 2: Recombinant Human CD34 Protein 5ngLane 3: Recombinant Human CD34 Protein 2.5ng
Anti-CD34 antibody, PA1334, IHC(P)IHC(P): Rat Kidney Tissue
Anti-CD34 antibody, PA1334, IHC(P)IHC(P): Human Lung Cancer Tissue
Anti-CD34 antibody, PA1334, IHC(F)IHC(F): Rat Kidney Tissue
Anti-CD34 antibody, PA1334, IHC(F)IHC(F): Rat Placenta Tissue
Figure 6. Flow Cytometry analysis of A431 cells using anti- CD34 antibody (PA1334). Overlay histogram showing A431 cells stained with PA1334 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti- CD34 Antibody (PA1334,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Figure 7. IHC analysis of CD34 using anti- CD34 antibody (PA1334). CD34 was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti- CD34 Antibody (PA1334) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
*if product is indicated to react with multiple species, protein info is based on the human gene.
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