Anti-CD34 Antibody

CD34 antibody

Boster Bio Anti-CD34 Antibody catalog # PA1334. Tested in Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 10 publication(s).

Product Info Summary

SKU: PA1334
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IHC, IHC-F, ICC, WB

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Product Name

Anti-CD34 Antibody

View all CD34 Antibodies

SKU/Catalog Number

PA1334

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CD34 Antibody catalog # PA1334. Tested in Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD34 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1334)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human CD34 (366-382aa QATSRNGHSARQHVVAD), identical to the related mouse and rat sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1334 is reactive to CD34 in Human, Mouse, Rat

Applications

PA1334 is guaranteed for Flow Cytometry, IHC, IHC-F, ICC, WB Boster Guarantee

Background of CD34

CD34 is a monomeric cell surface antigen with a molecular mass of approximately 110 KD.CD34 is expressed in humans in hematopoietic stem cells, vascular endothelium, and blasts from 30% of patients with acute myeloid and lymphocytic leukemia. The human CD34 gene spans 26 kb and has 8 exons, a structure quite similar to that of the murine gene. By Southern blot analysis of DNA from a panel of human x mouse somatic cell hybrids using a CD34 cDNA probe demonstrate that the gene for CD34 is located on human chromosome 1 in the 1q12----qter region. CD34 plays an important role in the formation of progenitor cells during both embryonic and adult hematopoiesis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Mouse, Rat, Human
Immunocytochemistry, 0.5-1μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For CD34 (Source: Uniprot.org, NCBI)

Uniprot ID

P28906

Gene ID

947

Gene Name

CD34

Full Name

Hematopoietic progenitor cell antigen CD34

Weight

40.716kDa

Superfamily

CD34 family

Alternative Names

CD34 antigenhematopoietic progenitor cell antigen CD34; CD34 molecule; CD34; HPCA1 CD34 CD34 molecule hematopoietic progenitor cell antigen CD34|CD34 antigen

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CD34, check out the CD34 Infographic

CD34 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CD34: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

PA1334 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

The role of 5‐HT2B receptors in mitral valvulopathy: bone marrow mobilization of endothelial progenitors

Dahai Dong,Yu Yao,Jinlei Song,Lijiang Sun,Guiming Zhang,"Cancer-Associated Fibroblasts Regulate Bladder Cancer Invasion and Metabolic Phenotypes through Autophagy",Disease Markers,vol.2021,Article ID 6645220,8 pages,2021.https://doi.org/10.1155/2021/6645220
Species: Human
PA1334 usage in article: APP:IF, SAMPLE:HFF CELL, DILUTION:NA

Ma C,Liu G,Liu W,Xu W,Li H,Piao S,Sui Y,Feng W.CXCL1 stimulates decidual angiogenesis via the VEGF-A pathway during the first trimester of pregnancy.Mol Cell Biochem.2021 Mar 26.doi:10.1007/s11010-021-04137-x.Epub ahead of print.PMID:33770315.
Species: Human,Mouse
PA1334 usage in article: APP:ICC, SAMPLE:DECIDUA TISSE, DILUTION:1:500

Shuyuan Guo,Yusen Zhang,Yanmin Zhang,Fanhua Meng,Minghua Li,Zhendong Yu,Yun Chen,Guanghui Cui, "Multiple Intravenous Injections of Valproic Acid-Induced Mesenchymal Stem Cell from Human-Induced Pluripotent Stem Cells Improved Cardiac Function in an Acute
Species: Human,Rat
PA1334 usage in article: APP:FLOW CYTOMETRY, SAMPLE:PBMC-5 CELL, DILUTION:NA

Nie X,Zhang H,Shi X,Zhao J,Chen Y,Wu F,Yang J,Li X.Asiaticoside nitric oxide gel accelerates diabetic cutaneous ulcers healing by activating Wnt/β-catenin signaling pathway.Int Immunopharmacol.2020 Feb;79: 106109.doi:10.1016/j.intimp.2019.106109.Epub 2019
Species: Human,Rat
PA1334 usage in article: APP:IHC, SAMPLE:DCU WOUND TISSUE, DILUTION:1:50

Chen YY,Wu XS,Tian YY,Zhao P,Sun SW,Yang CB.Evaluation of Femoral Head Perfusion by Contrast-Enhanced Ultrasound in a Rabbit Model of Steroid-Induced Osteonecrosis.J Ultrasound Med.2020 May;39(5):987-995.doi:10.1002/jum.15184.Epub 2019 Dec 2.PMID:31789475
Species: Rabbit
PA1334 usage in article: APP:IHC, SAMPLE:FEMORAL HEADS, DILUTION:NA

Assefa F,Lim J,Kim JA,Ihn HJ,Lim S,Nam SH,Bae YC,Park EK. Secretoneurin, a Neuropeptide, Enhances Bone Regeneration in a Mouse Calvarial Bone Defect Model. Tissue Eng Regen Med.2020 Nov 3.doi: 10.1007/s13770-020-00304-1.Epub ahead of print.PMID:33145742.
Species: Mouse
PA1334 usage in article: APP:IHC, SAMPLE:CALVARIAL BONE, DILUTION:1:500

SIRT1 participates in the response to methylglyoxal-dependent glycative stress in mouse oocytes and ovary Giovanna Di Emidio, et al. Biochim Biophys Acta Mol Basis Dis. 2019 Jun 1;1865(6):1389-1401. doi: 10.1016/j.bbadis.2019.02.011. Epub 2019 Feb 13.

Dl-3-n-Butylphthalide treatment enhances hemodynamics and ameliorates memory deficits in rats with chronic cerebral hypoperfusion

Dipalmitoylphosphatidic acid inhibits tumor growth in triple-negative breast cancer

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18 Customer Q&As for Anti-CD34 Antibody

Question

We are currently using anti-CD34 antibody PA1334 for human tissue, and we are happy with the IHC-F results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2020-04-29

Answer

The anti-CD34 antibody (PA1334) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-04-29

Question

We are interested in using your anti-CD34 antibody for paracrine signaling studies. Has this antibody been tested with western blotting on a431 cells? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-03-23

Answer

I appreciate your inquiry. This PA1334 anti-CD34 antibody is tested on rat placenta tissue, kidney tissue, lung cancer tissue, a431 cells. It is guaranteed to work for Flow Cytometry, IHC-P, IHC-F, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-03-23

Question

We bought anti-CD34 antibody for IHC-P on esophagus in the past. I am using human, and We are going to use the antibody for WB next. We need examining esophagus as well as placenta in our next experiment. Do you have any suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2020-03-13

Answer

I looked at the website and datasheets of our anti-CD34 antibody and it seems that PA1334 has been validated on human in both IHC-P and WB. Thus PA1334 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-03-13

Question

Our lab were happy with the WB result of your anti-CD34 antibody. However we have been able to see positive staining in bone marrow membrane using this antibody. Is that expected? Could you tell me where is CD34 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-03-04

Answer

Based on literature, bone marrow does express CD34. Generally CD34 expresses in membrane. Regarding which tissues have CD34 expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 1370171
Placenta, Pubmed ID: 14702039
Skin, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2020-03-04

Question

Would PA1334 anti-CD34 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-12-05

Answer

You can see on the product datasheet, PA1334 anti-CD34 antibody as been tested on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-12-05

Question

We need to test anti-CD34 antibody PA1334 on rat megakaryoblast for research purposes, then I may be interested in using anti-CD34 antibody PA1334 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-11-19

Answer

The products we sell, including anti-CD34 antibody PA1334, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-11-19

Question

Is a blocking peptide available for product anti-CD34 antibody (PA1334)?

Verified Customer

Verified customer

Asked: 2019-07-23

Answer

We do provide the blocking peptide for product anti-CD34 antibody (PA1334). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-07-23

Question

I performed a 1:500 dilution for the working solution of this antibody (500 µg/mL). The immunostaining signal looks weak. Please advice for the next step.

Verified Customer

Verified customer

Asked: 2019-07-08

Answer

In this case, try using a 1:200 dilution to see if the signal looks stronger.

Boster Scientific Support

Answered: 2019-07-08

Question

Would anti-CD34 antibody PA1334 work on bovine Flow Cytometry with placenta?

Verified Customer

Verified customer

Asked: 2019-07-04

Answer

Our lab technicians have not tested anti-CD34 antibody PA1334 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-CD34 antibody PA1334 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine placenta in Flow Cytometry, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-07-04

Question

Do you have a BSA free version of anti-CD34 antibody PA1334 available?

Verified Customer

Verified customer

Asked: 2018-12-03

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD34 antibody PA1334 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-12-03

Question

I see that the anti-CD34 antibody PA1334 works with ICC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-09-10

Answer

You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-09-10

Question

I have a question about product PA1334, anti-CD34 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-02-26

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1334 anti-CD34 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-02-26

Question

I was wanting to use your anti-CD34 antibody for ICC for rat megakaryoblast on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat megakaryoblast identification?

Verified Customer

Verified customer

Asked: 2017-10-27

Answer

As indicated on the product datasheet, PA1334 anti-CD34 antibody has been tested for Flow Cytometry, IHC-P, IHC-F, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat megakaryoblast in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-10-27

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for megakaryoblast using anti-CD34 antibody PA1334. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2017-10-23

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-10-23

Question

We have observed staining in mouse megakaryoblast. Do you have any suggestions? Is anti-CD34 antibody supposed to stain megakaryoblast positively?

J. Collins

Verified customer

Asked: 2016-09-30

Answer

From what I have seen in literature megakaryoblast does express CD34. From what I have seen in Uniprot.org, CD34 is expressed in esophagus, bone marrow, megakaryoblast, heart, placenta, skin, among other tissues. Regarding which tissues have CD34 expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 1370171
Placenta, Pubmed ID: 14702039
Skin, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2016-09-30

Question

Would anti-CD34 antibody PA1334 work for ICC with megakaryoblast?

D. Wu

Verified customer

Asked: 2014-09-11

Answer

According to the expression profile of megakaryoblast, CD34 is highly expressed in megakaryoblast. So, it is likely that anti-CD34 antibody PA1334 will work for ICC with megakaryoblast.

Boster Scientific Support

Answered: 2014-09-11

Question

Please see the WB image, lot number and protocol we used for megakaryoblast using anti-CD34 antibody PA1334. Please let me know if you require anything else.

B. Li

Verified customer

Asked: 2013-09-17

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-09-17

Question

Is this PA1334 anti-CD34 antibody reactive to the isotypes of CD34?

M. Rodriguez

Verified customer

Asked: 2013-07-18

Answer

The immunogen of PA1334 anti-CD34 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human CD34(366-382aa QATSRNGHSARQHVVAD), identical to the related mouse and rat sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2013-07-18

Size

Conjugation

Total: $315

SKU:PA1334

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PA1334
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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$50 fee for conjugation. Antibody size is reduced to 50ug.

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