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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum, plasma (heparin, EDTA) and saliva.|
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Human TIMP-1 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human TIMP-1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human TIMP1 in cell culture supernatants, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 5pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human TIMP-1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0520)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: C24-A207
15.6 pg/ml - 1,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0520 is reactive to TIMP1 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) and saliva.
EK0520 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of TIMP-1
The tissue inhibitor of metalloproteinases 1 (TIMP1) is also called erythroid-potentiating activity (EPA). The X-linked gene for human TIMP1 is expressed in some but not all inactive X-containing somatic-cell hybrids, suggesting that this gene is either prone to reactivation or variable in its inactivation. Purified EPA specifically stimulates human and murine cells of the erythroid lineage, unlike murine interleukin-3 (IL-3) which stimulates precursor cells from all haematopoietic lineages. TIMP1 is thought to play a regulatory role in connective tissues by forming inactive complexes with those metalloproteinases that are normally responsible for connective tissue turnover. The human gene encoding TIMP has been mapped to the X chromosome in the region Xp11.1-p11.4. The standard product used in this kit is natural TIMP-1 with the molecular mass of 22KDa.
|EK0520-CAP||Anti-Human TIMP1 Pre-coated 96-well strip microplate||1|
|EK0520-ST||Human TIMP1 Standard||2 vials, 10 ng/tube|
|EK0520-DA||Human TIMP1 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human TIMP-1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect TIMP-1, Dilution ratio of 1:100, concentration in serum is around 150ng/ml.
Some articles we found to cite concentrations of TIMP-1 in samples: 24078814 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human TIMP-1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of TIMP-1 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For TIMP1 (Source: Uniprot.Org, NCBI)
Metalloproteinase inhibitor 1
protease inhibitor I35 (TIMP) family
CLGI; Collagenase inhibitor; collagenase inhibitor); EPATIMP-1; EPO; erythroid potentiating activity; Erythroid-potentiating activity; Fibroblast collagenase inhibitor; FLJ90373; HCI; metalloproteinase inhibitor 1; TIMP metallopeptidase inhibitor 1; TIMP1; TIMP-1; TIMPtissue inhibitor of metalloproteinase 1 (erythroid potentiating activity; Tissue inhibitor of metalloproteinases 1 TIMP1 CLGI, EPA, EPO, HCI, TIMP, TIMP-1 TIMP metallopeptidase inhibitor 1 metalloproteinase inhibitor 1|collagenase inhibitor|epididymis secretory sperm binding protein|erythroid potentiating activity|fibroblast collagenase inhibitor|tissue inhibitor of metalloproteinases 1*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on TIMP1, check out the TIMP1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for TIMP1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0520 has been cited in 16 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Cosentino G,Di Stefano V,Presti RL,Montana M,Todisco M,Gastaldi M,Cortese A,Alfonsi E,Tassorelli C,Fierro B,Caimi G,Brighina F.Expression pattern of matrix metalloproteinases-2 and -9 and their tissue inhibitors in patients with chronic inflammatory demyelinating polyneuropathy.Neurol Sci.2021 May 14.doi:10.1007/s10072-021-05314-y.Epub ahead of print.PMID:33988800.
EK0520 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Yu WY,Cai W,Ying HZ, Zhang WY,Zhang HH,Yu CH.Exogenous Plant gma-miR-159a, Identified by miRNA Library Functional Screening, Ameliorated Hepatic Stellate Cell Activation and Inflammation via Inhibiting GSK-3β-Mediated Pathways.J Inflamm Res.2021;14:2157-2172https://doi.org/10.2147/JIR.S304828
EK0520 usage in article: APP:ELISA, SAMPLE:HSCS, DILUTION:NA
The tissue inhibitor of metalloproteinases-1 (TIMP-1) promotes survival and migration of acute myeloid leukemia cells through CD63/PI3K/Akt/p21 signaling
Crucial factors of the inflammatory microenvironment (IL-1?/TNF-?/TIMP-1) promote the maintenance of the malignant hemopoietic clone of myelofibrosis: an in vitro study
Increased expression of matrix metalloproteinase-9 associated with gastric ulcer recurrence
Cavdar Z, Ozbal S, Celik A, Ergur Bu, Guneli E, Ural C, Camsari T, Guner Ga. Biotech Histochem. 2014 May;89(4):304-14. Doi: 10.3109/10520295.2013.847498. Epub 2013 Oct 28. The Effects Of Alpha-Lipoic Acid On Mmp-2 And Mmp-9 Activities In A Rat Ren...
Liu J, Zhong X, He Z, Wei L, Zheng X, Zhang J, Bai J, Zhong W, Zhong D. Mediators Inflamm. 2014;2014:708608. Doi: 10.1155/2014/708608. Epub 2014 Nov 4. Effect Of Low-Dose, Long-Term Roxithromycin On Airway Inflammation And Remodeling Of Stable Non...
Simmers P, Gishto A, Vyavahare N, Kothapalli Cr. Tissue Eng Part A. 2015 Apr;21(7-8):1455-70. Doi: 10.1089/Ten.Tea.2014.0363. Epub 2015 Mar 9. Nitric Oxide Stimulates Matrix Synthesis And Deposition By Adult Human Aortic Smooth Muscle Cells Within...
Xu Xl, Ling Dy, Zhu Qy, Fan Wj, Zhang W. Eur J Pharmacol. 2013 Jan 5;698(1-3):370-8. Doi: 10.1016/J.Ejphar.2012.11.019. Epub 2012 Nov 21. The Effect Of 2,3,4',5-Tetrahydroxystilbene-2-0-??-D Glucoside On Neointima Formation In A Rat Artery Balloon...
Deng Cl, Ling St, Liu Xq, Zhao Yj, Lv Yf. Exp Ther Med. 2015 Mar;9(3):992-998. Epub 2015 Jan 20. Decreased Expression Of Matrix Metalloproteinase-1 In The Maternal Umbilical Serum, Trophoblasts And Decidua Leads To Preeclampsia.
Stio M, Martinesi M, Treves C, Borgioli F. J Mater Sci Mater Med. 2015 Feb;26(2):100. Doi: 10.1007/S10856-015-5446-Y. Epub 2015 Feb 6. In Vitro Response Of Human Peripheral Blood Mononuclear Cells To Aisi 316L Austenitic Stainless Steel Subjected ...
Hopps E, Lo Presti R, Montana M, Noto D, Averna Mr, Caimi G. J Investig Med. 2013 Aug;61(6):978-83. Doi: 10.231/Jim.0B013E318294E9Da. Gelatinases And Their Tissue Inhibitors In A Group Of Subjects With Metabolic Syndrome.
Hopps E, Lo Presti R, Montana M, Canino B, Averna Mr, Caimi G. Mediators Inflamm. 2014;2014:510619. Doi: 10.1155/2014/510619. Epub 2014 Jul 10. Study Of The Correlations Among Some Parameters Of The Oxidative Status, Gelatinases, And Their Inhibit...
Gishto A, Farrell K, Kothapalli Cr. J Biomed Mater Res A. 2015 Feb;103(2):693-708. Doi: 10.1002/Jbm.A.35217. Epub 2014 May 19. Tuning Composition And Architecture Of Biomimetic Scaffolds For Enhanced Matrix Synthesis By Murine Cardiomyocytes.
Kim Ks, Lee Ya, Choi Hm, Yoo Mc, Yang Hi. Rheumatol Int. 2012 Oct;32(10):3069-75. Epub 2011 Sep 13. Implication Of Mmp-9 And Urokinase Plasminogen Activator (Upa) In The Activation Of Pro-Matrix Metalloproteinase (Mmp)-13.
Wang B, Liang S, Wang Y, Zhu Xq, Gong W, Zhang Hq, Li Y, Xia Cm. Plos Negl Trop Dis. 2015 Jan 15;9(1):E0003434. Doi: 10.1371/Journal.Pntd.0003434. Ecollection 2015. Th17 Down-Regulation Is Involved In Reduced Progression Of Schistosomiasis Fibrosi...
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