Human TNFSF11/RANKL ELISA Kit PicoKine™

Human TNFSF11/RANKL ELISA Kit PicoKine™ (96 Tests). Quantitate Human TNFSF11 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml. Cited in 10 publication(s).

Product Info Summary

SKU: EK0842
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human TNFSF11/RANKL ELISA Kit PicoKine™

See all TRANCE/TNFSF11/RANK L products

SKU/Catalog Number

EK0842

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human TNFSF11/RANKL ELISA Kit PicoKine™ (96 Tests). Quantitate Human TNFSF11 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human TNFSF11/RANKL ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0842)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: G64-D245

Sensitivity

<10 pg/ml

Assay Range

78 pg/ml - 5,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0842 is reactive to TNFSF11 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0842 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0842-CAP Anti-Human TNFSF11 Pre-coated 96-well strip microplate 1
EK0842-ST Human TNFSF11 Standard 2 vials, 10 ng/tube
EK0842-DA Human TNFSF11 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)078156313625125025005000
O.D.0.0340.1390.1510.2620.4530.7831.3311.926

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect TRANCE/TNFSF11/RANK L, Dilution ratio of 1:1, concentration in serum is target in serum is below detection limit..

Some articles we found to cite concentrations of TRANCE/TNFSF11/RANK L in samples: 22057136 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human TNFSF11/RANKL ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)11586522421147972279
Standard deviation5.5253.63105.374.7258.97116.22
CV (%)4.8%6.2%4.7%5.2%7.4%5.1%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of TRANCE/TNFSF11/RANK L in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11151261171221204.33.5%
Sample 286574684880481546.015.6%
Sample 32242222522562223223613.460.6%
*number of samples for each test n=16.

Gene/Protein Information For TNFSF11 (Source: Uniprot.Org, NCBI)

Uniprot ID

O14788

Gene ID

8600

Gene Name

TNFSF11

Full Name

Tumor necrosis factor ligand superfamily member 11

Weight

35.478kDa

Superfamily

tumor necrosis factor family

Alternative Names

CD254 antigen; CD254; ODF; OPGL; OPGLOPTB2; Osteoclast differentiation factor; Osteoprotegerin ligand; RANK L; RANKL; RANKLreceptor activator of nuclear factor kappa B ligand; Receptor activator of nuclear factor kappa-B ligand; sOdf; TNF-related activation-induced cytokine; TNFSF11; TRANCE; TRANCEODFhRANKL2; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand superfamily member 11 TNFSF11 CD254, ODF, OPGL, OPTB2, RANKL, TNLG6B, TRANCE, hRANKL2, sOdf TNF superfamily member 11 tumor necrosis factor ligand superfamily member 11|TNF-related activation-induced cytokine|osteoclast differentiation factor|osteoprotegerin ligand|receptor activator of nuclear factor kappa B ligand|tumor necrosis factor (ligand) superfamily, member 11|tumor necrosis factor ligand 6B|tumor necrosis factor superfamily member 11

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on TNFSF11, check out the TNFSF11 Infographic

TNFSF11 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for TNFSF11: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0842 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

HIF1α inhibition facilitates Leflunomide-AHR-CRP signaling to attenuate bone erosion in CRP-aberrant rheumatoid arthritis
Species: Human

Colombo MV, Bersini S, Arrigoni C, Gilardi M, Sansoni V, Ragni E, Candiani G, Lombardi G, Moretti M. Engineering the early bone metastatic niche through human vascularized immuno bone minitissues. Biofabrication. 2021 Mar 18. doi: 10.1088/1758-5090/abefea. Epub ahead of print. PMID: 33735854.
Species: Human,Mouse
EK0842 usage in article: APP:ELISA, SAMPLE:HUVECS, DILUTION

HIF1α inhibition facilitates Leflunomide-AHR-CRP signaling to attenuate bone erosion in CRP-aberrant rheumatoid arthritis

An in vitro 3D bone metastasis model by using a human bone tissue culture and human sex-related cancer cells

Tang Y, Sun F, Li X, Zhou Y, Yin S, Zhou X. J Endod. 2011 Dec;37(12):1653-8. Doi: 10.1016/J.Joen.2011.08.015. Epub 2011 Oct 2. Porphyromonas Endodontalis Lipopolysaccharides Induce Rankl By Mouse Osteoblast In A Way Different From That Of Escheric...

Zhao H, Ning Ll, Wang Zy, Li Ht, Qiao D, Yao Y, Qin Hl. Cell Biochem Biophys. 2014 Nov;70(2):1097-104. Doi: 10.1007/S12013-014-0028-Z. Calcitonin Gene-Related Peptide Inhibits Osteolytic Factors Induced By Osteoblast In Co-Culture System With Brea...

Tang Zn, Zhang F, Tang P, Qi Xw, Jiang J. Biochem Biophys Res Commun. 2011 May 13;408(3):411-6. Doi: 10.1016/J.Bbrc.2011.04.035. Epub 2011 Apr 13. Hypoxia Induces Rank And Rankl Expression By Activating Hif-1?? In Breast Cancer Cells.

Montesi M, Panseri S, Iafisco M, Adamiano A, Tampieri A. Plos One. 2015 Jul 6;10(7):E0132633. Doi: 10.1371/Journal.Pone.0132633. Ecollection 2015. Coupling Hydroxyapatite Nanocrystals With Lactoferrin As A Promising Strategy To Fine Regulate Bone ...

Xu S, Wang Y, Lu J, Xu J. Rheumatol Int. 2012 Nov;32(11):3397-403. Doi: 10.1007/S00296-011-2175-5. Epub 2011 Nov 6. Osteoprotegerin And Rankl In The Pathogenesis Of Rheumatoid Arthritis-Induced Osteoporosis.

Chen B, Li Xd, Liu Dx, Wang H, Xie P, Liu Zy, Hou Gq, Chang B, Du Sx. Phytomedicine. 2012 Aug 15;19(11):1029-34. Doi: 10.1016/J.Phymed.2012.06.002. Epub 2012 Jul 19. Canonical Wnt Signaling Is Required For Panax Notoginseng Saponin-Mediated Attenu...

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32 Customer Q&As for Human TNFSF11/RANKL ELISA Kit PicoKine™

Question

Q: how can I thaw whole blood sample for TRANCE ELISA after freezing?

Verified Customer

Verified customer

Asked: 2020-10-21

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test TRANCE for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2020-10-21

Question

Q: is it okay to use citrate plasma as samples in Human TRANCE Picokine® ELISA Kit (Catalog # EK0842)?

H. Krishna

Verified customer

Asked: 2020-10-18

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of TRANCE causing degradation of its protein structure. TRANCE may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the TRANCE ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2020-10-18

Question

Does EK0842 have cross-reactivity with mouse and rat samples? Considering the homology between these species is relatively high and the kit is using polyclonal antibodies, does your comment: "There is no detectable cross-reactivity with other relevant proteins." refer to other human proteins in the sample (this is how I read this) or does this include cross-reactivity with other species?

Verified customer

Asked: 2020-06-29

Answer

Our lab tested recombinant mouse and rat TNFSF11 protein using Human TNFSF11/RANKL ELISA Kit PicoKine™ (EK0842) and didn't observe any cross-reactivity. "There is no detectable cross-reactivity with other relevant proteins." refer to other human proteins in the sample and cross-reactivity with other species we tested as well.

Boster Scientific Support

Answered: 2020-06-30

Question

Can you please provide any additional details regarding the immunogen for the capture and detector for the EK0842 Human TNFSF11 ELISA Kit (RANKL)? If not can you provide the homology with cow for the immunogens of the capture and detector and do you know if the EK0842 Human TNFSF11 ELISA Kit (RANKL) would detect the cow RANKL?

Verified customer

Asked: 2020-06-15

Answer

The immunogen sequence of both the capture antibody and detection antibody for the Human TNFSF11/RANKL ELISA Kit PicoKine™ (EK0842) is Q73-D317 Accession num. O14788 Please click the following link for homology with cow: https://www.uniprot.org/blast/uniprot/B20200616E5A08BB0B2D1C45B0C7BC3B55FD265561A34CE1?fil=organism:9913 Our lab hasn't detected the cow RANKL using EK0842.

Boster Scientific Support

Answered: 2020-06-15

Question

Q: how to proceed with the analysis of ELISA data? I have obtained TRANCE level in plasma.

V. Gonzalez

Verified customer

Asked: 2020-05-11

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2020-05-11

Question

Do you have any information whether this kit could detect both cytoplasm and membrane bound RANKL?

Verified Customer

Verified customer

Asked: 2019-12-25

Answer

According to the immunogen sequence G64-D245 of this kit, it can only detect extracellular secreted protein.

Boster Scientific Support

Answered: 2019-12-25

Question

We recently used your ELISA kit (EK0842) for detecting RANKL protein from cell lysate. 1. Would you have a relevant protocol/literatures where they looked at cell lysate/membrane bound RANKL? 2. We were wondering if you would recommend using protease inhibitor for this type of ELISA? 3. We lysed our samples in deionised H2O by freeze-thaw cycle, could you check if this step is suitable? 4. We were thinking if we should centrifuge the cell lysate before we perform the ELISA? Please could you clarify these steps?

Verified Customer

Verified customer

Asked: 2019-12-16

Answer

Please see the following answers for the questions. 1. Would you have a relevant protocol/literatures where they looked at cell lysate/membrane bound RANKL? Preparation of lysate from cell culture (Protein Extraction from Cultured Cells) 1. Rinse cells in ice-cold PBS. 2. Scrape cells or trypsinize cells and wash with PBS. 3. Centrifuge at ~3000 x g for 2-3 minutes, remove PBS. The centrifugation force and time can be varied depending on the cell type. 4. Add IP lysis buffer(Boster Cat#AR0107). 5. Sonicate for 10

Boster Scientific Support

Answered: 2019-12-16

Question

When performing initial estimation of sample concentration (page 10 protocol) for EK0842, how should the remaining empty wells be protected while going through the steps of the protocol?

Verified customer

Asked: 2019-12-04

Answer

For the Human TNFSF11/RANKL ELISA Kit PicoKine™ (EK0842), you can strip off the plate and use the required number of wells only. Meanwhile I can follow up with our labs and address your question about protecting the empty wells.

Boster Scientific Support

Answered: 2019-12-04

Question

I want to know whether your product EK0842 is from human or mouse?

Verified Customer

Verified customer

Asked: 2019-11-27

Answer

The product EK0842 is a full sequence from human.

Boster Scientific Support

Answered: 2019-11-27

Question

What is the host for the capture and detection antibody for EK0842?

Verified customer

Asked: 2019-10-03

Answer

The host for the capture and detection antibody for the Human TNFSF11/RANKL ELISA Kit PicoKine™ (EK0842) is goat polyclonal.

Boster Scientific Support

Answered: 2019-10-03

Question

What is the capture purification method for EK0842?

Verified customer

Asked: 2019-10-03

Answer

For the Human TNFSF11/RANKL ELISA Kit PicoKine™ (EK0842), the capture purification is Affinity Purified.

Boster Scientific Support

Answered: 2019-10-03

Question

What is the detection purification method for EK0842?

Verified customer

Asked: 2019-10-03

Answer

For the Human TNFSF11/RANKL ELISA Kit PicoKine™ (EK0842), the detection purification is Affinity Purified.

Boster Scientific Support

Answered: 2019-10-03

Question

What is the standard source and expression for EK0842?

Verified customer

Asked: 2019-10-03

Answer

For the Human TNFSF11/RANKL ELISA Kit PicoKine™ (EK0842), the standard source is Recombinant Protein and expression is NSO.

Boster Scientific Support

Answered: 2019-10-03

Question

What is the immunogen for the capture antibody of EK0842?

Verified customer

Asked: 2019-10-03

Answer

The immunogen for the capture antibody of the Human TNFSF11/RANKL ELISA Kit PicoKine™ (EK0842) is G64-D245.

Boster Scientific Support

Answered: 2019-10-03

Question

Can EK0842 detect free RANKL and RANKL bound to osteoprotergerin?<br>Keywords: free, bound, osteoprotergerin

Verified Customer

Verified customer

Asked: 2019-09-24

Answer

Yes, EK0842 can detect free RANKL and RANKL bound to osteoprotergerin.

Boster Scientific Support

Answered: 2019-09-24

Question

Can EK0842 kit detect both cytoplasm and membrane bound RANKL?

Verified customer

Asked: 2019-08-22

Answer

The Human TNFSF11/RANKL ELISA Kit PicoKine EK0842 can only detect extracellular secreted protein. The secreted protein is synthesized in the intracellular space of cells.

Boster Scientific Support

Answered: 2019-08-27

Question

The customer replied with the following, please assist. "You mean this kit can't it detect cytoplasm and membrane bound RANKL? Why does it only detect extracellular secreted protein? And Why Do You Guarantee Cell Lysates? It Won't Include Secreted Protein?"

Verified Customer

Verified customer

Asked: 2019-08-19

Answer

The secreted protein is synthesized in the intracellular space of cells. And the kit is able to detect patial intracellular secreted protein which hasn't released into extracellular space.

Boster Scientific Support

Answered: 2019-08-19

Question

For these two kits: Human TNFSF11/RANKL PicoKine

Verified Customer

Verified customer

Asked: 2019-07-30

Answer

Our lab technician suggest to use serum. Please see the following expected range for plasma and serum from healthy human.

Boster Scientific Support

Answered: 2019-07-30

Question

Would you recommend using protease inhibitor for this type of ELISA?

Verified customer

Asked: 2019-07-26

Answer

We recommend using a Protease inhibitor for the Human TNFSF11/RANKL ELISA Kit PicoKine™ (EK0842).

Boster Scientific Support

Answered: 2019-07-29

Question

Would lysing samples in deionised H2O by freeze-thaw cycle be a suitable step for EK0842?

Verified customer

Asked: 2019-07-26

Answer

Lysing samples in deionised H2O by freeze-thaw cycle is not suitable for the Human TNFSF11/RANKL ELISA Kit PicoKine™ (EK0842).

Boster Scientific Support

Answered: 2019-07-29

Question

Is there a need to centrifuge the cell lysate before perfoming the ELISA?

Verified customer

Asked: 2019-07-26

Answer

Please centrifuge the cell lysate before performing the ELISA.

Boster Scientific Support

Answered: 2019-07-29

Question

Would you have a relevant protocol/literatures where they looked at cell lysate/membrane bound RANKL?

Verified customer

Asked: 2019-07-26

Answer

We haven't found any literatures where they looked at the cell lysate/membrane bound RANKL. Here is a preparation protocol for your reference: Preparation of lysate from cell culture (Protein Extraction from Cultured Cells) 1. Rinse cells in ice-cold PBS. 2. Scrape cells or trypsinize cells and wash with PBS. 3. Centrifuge at ~3000 x g for 2-3 minutes, remove PBS. The centrifugation force and time can be varied depending on the cell type. 4. Add IP lysis buffer(Boster AR0107). 5. Sonicate for 10–15 sec to complete cell lysis. 6. Centrifuge at ~10000 x g for 10 minutes. The centrifugation force and time can be varied depending on the cell type. 7. Aspirate supernatant avoiding pellet and upper, lipid-containing phase. 8. Aliquot supernatant and store at -20°C.

Boster Scientific Support

Answered: 2019-07-29

Question

Q: can you suggest the dilution ratio of serum samples for detection of TRANCE in Human plasma? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.

P. Hernandez

Verified customer

Asked: 2019-06-14

Answer

A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the TRANCE ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2019-06-14

Question

What cells were tested with this kit and how ere the cell lysate samples prepared?

Verified Customer

Verified customer

Asked: 2019-03-20

Answer

Human peripheral blood mononuclear cells (PBMCs) lysate was tested. RIPA lysis buffer was used at 1:5 by cell volume ratio, and the dilution is 1:1.

Boster Scientific Support

Answered: 2019-03-20

Question

Q: What is the optimal O.D. value for TRANCE ELISA kit? I performed your TRANCE ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain TRANCE even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2018-08-18

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this TRANCE ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of TRANCE that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2018-08-18

Question

I just want to know about Human TNFSF11/RANKL ELISA Kit; there were studies that detects endogenous levels of total RANKL, so does it mean that the antibody identifies all the three isoforms either in soluble and membrane forms?

Verified Customer

Verified customer

Asked: 2018-04-19

Answer

The Human TNFSF11/RANKL ELISA Kit binds to the C-terminal extracellular topological domain of isoforms 1, 2, and 3 (based on prediction). The amino acid range of this immunogen is aa 60-110 of human TNF ligand super family member 11 with accession number O14788 (http://www.uniprot.org/uniprot/O14788).

Boster Scientific Support

Answered: 2018-04-19

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

B. Perez

Verified customer

Asked: 2018-01-08

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2018-01-08

Question

To Boster Technical Support: We ordered your product EK0842 Human TNFSF11/RANKL ELISA Kit for protein stimulation, however we could not see information about the concentration/dilution conditions for the treatment. Could you give the approximate amounts of RANKL to use?

Verified Customer

Verified customer

Asked: 2017-07-28

Answer

We tested the activity of EK0842 Human TNFSF11/RANKL ELISA Kit by measuring the induction of NFkappaB and 50% of its maximum conduction reached 10-25ng/ml of RANKL.

Boster Scientific Support

Answered: 2017-07-28

Question

Q: if the enzyme conjugated TRANCE antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the TRANCE antigen?

Verified Customer

Verified customer

Asked: 2016-02-24

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2016-02-24

Question

What is the specificity of the RANKL antibody? (i.e. Which epitopes are recognized?)

Verified Customer

Verified customer

Asked: 2015-05-19

Answer

The standard for this antibody is recombinant human TNFSF11 (NSO, G64-D245). Therefore, the ELISA kit can detect the soluble forms derived from the cleavage of membrane-bound protein (aa. 140-317) or alternative splicing (aa. 74-317).

Boster Scientific Support

Answered: 2015-05-19

Question

Q: Can TRANCE ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

M. Chen

Verified customer

Asked: 2015-02-07

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2015-02-07

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring TRANCE using the EK0842 Human TRANCE Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2014-02-05

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.

Boster Scientific Support

Answered: 2014-02-05

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