Human TNFSF11/RANKL ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TNFSF11/RANKL. 96wells/kit, with removable strips. Cited in 8 publication(s).
Human TNFSF11/RANKL ELISA Kit PicoKine™ Info At A Glance
|Size:||96wells/kit, with removable strips.|
|Sample Type:||cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).|
|Sample Volume:||100μl per well|
|Product Name||Human TNFSF11/RANKL ELISA Kit PicoKine™
See all TRANCE/TNFSF11/RANK L primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||Detect Human Rankl/TNFSF11 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Rankl/TNFSF11 tissue specificity: Highest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid.|
|Cite This Product||Human TNFSF11/RANKL ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0842)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).|
|Immunogen/Standard||Expression system for standard: NSO; Immunogen sequence: G64-D245|
|Antibody Clonalities||Capture antibody|Detection antibody
polyclonal antibody from goat|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96wells/kit, with removable strips.|
Gene/Protein Basic Information For TNFSF11 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||8600|
|Species Of This Entry||Human|
|Protein Name||Tumor necrosis factor ligand superfamily member 11|
|Superfamily||tumor necrosis factor family|
|Alternative Names||TRANCE/TNFSF11/RANK L|CD254 antigen; CD254; ODF; OPGL; OPGLOPTB2; Osteoclast differentiation factor; Osteoprotegerin ligand; RANK L; RANKL; RANKLreceptor activator of nuclear factor kappa B ligand; Receptor activator of nuclear factor kappa-B ligand; sOdf; TNF-related activation-induced cytokine; TNFSF11; TRANCE; TRANCEODFhRANKL2; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand superfamily member 11|
|Post Tranlational Modifications||Phosphorylation; Cleavage; Ubiquitination; Acetylation; Methylation; Dephosphorylation; Prenylation; Glycosylation; Oxidation; Geranylgeranylation; Demethylation; Sumoylation; Reduction; Farnesylation|
|Subcellular Localization||[Isoform 1]: Cell membrane; Single-pass type II membrane protein.; [Isoform 3]: Cell membrane; Single-pass type II membrane protein.; [Isoform 2]: Cytoplasm.; [Tumor necrosis factor ligand superfamily member 11, soluble form]: Secreted.|
|Gene Location||13q14.11, on Chromasome 13, gene sequence: NC_000013.11 (42562736..42608013)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For TNFSF11 (Source: Uniprot.org, NCBI)
|Protein Function||Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy (PubMed:22664871). Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca (2+) resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts. During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation (By similarity).|
|Tissue Specificity||Highest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid.|
|Interacting Proteins||TNFAIP6; TNFRSF11A; TNFRSF11B|
|Related Diseases||Osteoporosis; Osteopenia; Arthritis; Bone Diseases; Rheumatoid Arthritis; Malignant Neoplasms; Neoplasms; Osteolysis; Inflammation; Bone Neoplasms; Neoplasm Metastasis; Multiple Myeloma; Fracture; Metastatic Malignant Neoplasm To The Bone; Osteoporosis, Postmenopausal; Albers-schonberg Disease; Trance State; Periodontitis; Metabolic Bone Disorder; Malignant Neoplasm Of Breast|
|Related Pathways||Bone Resorption; Osteoclast Differentiation; Bone Remodeling; Pathogenesis; Secretion; Osteoclast Development; Osteoblast Differentiation; Cell Proliferation; Localization; Cell Growth; Immune Response; Cell Differentiation; Cytokine Production; Bone Development; Cell Death; Menopause; Regulation Of Bone Resorption; Transport; Aging; Reverse Transcription|
|Background||Receptor activator of nuclear factor kappa-B ligand(RANKL), also known as tumor necrosis factor ligand superfamily member 11(TNFSF11), is a protein that in humans is encoded by the TNFSF11 gene. This gene encodes a member of the tumor necrosis factor(TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This gene is mapped to chromosome13q14.11. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. This gene may play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.|
|Scientific References||PMID: 9367155 by Anderson D.M., et al. A homologue of the TNF receptor and its ligand enhance T-cell growth and dendritic-cell function.|
PMID: 9568710 by Lacey D.L., et al. Osteoprotegerin ligand is a cytokine that regulates osteoclast differentiation and activation.
Kit Components And Assay QC Data Details Of Human TNFSF11/RANKL ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK0842-CAP||96-well plate precoated with anti-Human TRANCE/TNFSF11/RANK L antibody||1|
|EK0842-ST||lyophilized recombinant Human TRANCE/TNFSF11/RANK L standard||10ng/tube|
|EK0842-DA||biotinylated anti-Human TRANCE/TNFSF11/RANK L antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0842 Human TNFSF11/RANKL ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Human TNFSF11/RANKL ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Human TNFSF11/RANKL ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Total number of citations: 8
|Chen B, Li Xd, Liu Dx, Wang H, Xie P, Liu Zy, Hou Gq, Chang B, Du Sx. Phytomedicine. 2012 Aug 15;19(11):1029-34. Doi: 10.1016/J.Phymed.2012.06.002. Epub 2012 Jul 19. Canonical Wnt Signaling Is Required For Panax Notoginseng Saponin-Mediated Attenu...||PubMed ID 22818895|
|Xu S, Wang Y, Lu J, Xu J. Rheumatol Int. 2012 Nov;32(11):3397-403. Doi: 10.1007/S00296-011-2175-5. Epub 2011 Nov 6. Osteoprotegerin And Rankl In The Pathogenesis Of Rheumatoid Arthritis-Induced Osteoporosis.||PubMed ID 22057136|
|Montesi M, Panseri S, Iafisco M, Adamiano A, Tampieri A. Plos One. 2015 Jul 6;10(7):E0132633. Doi: 10.1371/Journal.Pone.0132633. Ecollection 2015. Coupling Hydroxyapatite Nanocrystals With Lactoferrin As A Promising Strategy To Fine Regulate Bone ...||PubMed ID 26148296|
|Tang Zn, Zhang F, Tang P, Qi Xw, Jiang J. Biochem Biophys Res Commun. 2011 May 13;408(3):411-6. Doi: 10.1016/J.Bbrc.2011.04.035. Epub 2011 Apr 13. Hypoxia Induces Rank And Rankl Expression By Activating Hif-1?? In Breast Cancer Cells.||PubMed ID 21514280|
|Zhao H, Ning Ll, Wang Zy, Li Ht, Qiao D, Yao Y, Qin Hl. Cell Biochem Biophys. 2014 Nov;70(2):1097-104. Doi: 10.1007/S12013-014-0028-Z. Calcitonin Gene-Related Peptide Inhibits Osteolytic Factors Induced By Osteoblast In Co-Culture System With Brea...||PubMed ID 24853718|
|Tang Y, Sun F, Li X, Zhou Y, Yin S, Zhou X. J Endod. 2011 Dec;37(12):1653-8. Doi: 10.1016/J.Joen.2011.08.015. Epub 2011 Oct 2. Porphyromonas Endodontalis Lipopolysaccharides Induce Rankl By Mouse Osteoblast In A Way Different From That Of Escheric...||PubMed ID 22099899|
|An in vitro 3D bone metastasis model by using a human bone tissue culture and human sex-related cancer cells||PubMed ID 27765913|
|HIF1α inhibition facilitates Leflunomide-AHR-CRP signaling to attenuate bone erosion in CRP-aberrant rheumatoid arthritis||PubMed ID 31594926|
Order Product (EK0842)
Domestically in USA and Canada, ships next business day if available in stock.
All Boster antibodies and ELISA kits are guaranteed to meet the specifications on the data sheet.See Details
Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.Download Free PDFs Now
Guaranteed product quality
We promise all of our products perform as described in datasheets.