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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
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Human TNFSF11/RANKL ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human TNFSF11/RANKL ELISA Kit PicoKine™ (96 Tests). Quantitate Human TNFSF11 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Human TNFSF11/RANKL ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0842)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: G64-D245
78 pg/ml - 5,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0842 is reactive to TNFSF11 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
EK0842 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK0842-CAP||Anti-Human TNFSF11 Pre-coated 96-well strip microplate||1|
|EK0842-ST||Human TNFSF11 Standard||2 vials, 10 ng/tube|
|EK0842-DA||Human TNFSF11 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human TNFSF11/RANKL PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect TRANCE/TNFSF11/RANK L, Dilution ratio of 1:1, concentration in serum is target in serum is below detection limit..
Some articles we found to cite concentrations of TRANCE/TNFSF11/RANK L in samples: 22057136 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human TNFSF11/RANKL ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of TRANCE/TNFSF11/RANK L in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For TNFSF11 (Source: Uniprot.Org, NCBI)
Tumor necrosis factor ligand superfamily member 11
tumor necrosis factor family
CD254 antigen; CD254; ODF; OPGL; OPGLOPTB2; Osteoclast differentiation factor; Osteoprotegerin ligand; RANK L; RANKL; RANKLreceptor activator of nuclear factor kappa B ligand; Receptor activator of nuclear factor kappa-B ligand; sOdf; TNF-related activation-induced cytokine; TNFSF11; TRANCE; TRANCEODFhRANKL2; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand superfamily member 11 TNFSF11 CD254, ODF, OPGL, OPTB2, RANKL, TNLG6B, TRANCE, hRANKL2, sOdf TNF superfamily member 11 tumor necrosis factor ligand superfamily member 11|TNF-related activation-induced cytokine|osteoclast differentiation factor|osteoprotegerin ligand|receptor activator of nuclear factor kappa B ligand|tumor necrosis factor (ligand) superfamily, member 11|tumor necrosis factor ligand 6B|tumor necrosis factor superfamily member 11*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on TNFSF11, check out the TNFSF11 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for TNFSF11: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0842 has been cited in 9 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Colombo MV, Bersini S, Arrigoni C, Gilardi M, Sansoni V, Ragni E, Candiani G, Lombardi G, Moretti M. Engineering the early bone metastatic niche through human vascularized immuno bone minitissues. Biofabrication. 2021 Mar 18. doi: 10.1088/1758-5090/abefea. Epub ahead of print. PMID: 33735854.
EK0842 usage in article: APP:ELISA, SAMPLE:HUVECS, DILUTION
HIF1α inhibition facilitates Leflunomide-AHR-CRP signaling to attenuate bone erosion in CRP-aberrant rheumatoid arthritis
An in vitro 3D bone metastasis model by using a human bone tissue culture and human sex-related cancer cells
Tang Y, Sun F, Li X, Zhou Y, Yin S, Zhou X. J Endod. 2011 Dec;37(12):1653-8. Doi: 10.1016/J.Joen.2011.08.015. Epub 2011 Oct 2. Porphyromonas Endodontalis Lipopolysaccharides Induce Rankl By Mouse Osteoblast In A Way Different From That Of Escheric...
Zhao H, Ning Ll, Wang Zy, Li Ht, Qiao D, Yao Y, Qin Hl. Cell Biochem Biophys. 2014 Nov;70(2):1097-104. Doi: 10.1007/S12013-014-0028-Z. Calcitonin Gene-Related Peptide Inhibits Osteolytic Factors Induced By Osteoblast In Co-Culture System With Brea...
Tang Zn, Zhang F, Tang P, Qi Xw, Jiang J. Biochem Biophys Res Commun. 2011 May 13;408(3):411-6. Doi: 10.1016/J.Bbrc.2011.04.035. Epub 2011 Apr 13. Hypoxia Induces Rank And Rankl Expression By Activating Hif-1?? In Breast Cancer Cells.
Montesi M, Panseri S, Iafisco M, Adamiano A, Tampieri A. Plos One. 2015 Jul 6;10(7):E0132633. Doi: 10.1371/Journal.Pone.0132633. Ecollection 2015. Coupling Hydroxyapatite Nanocrystals With Lactoferrin As A Promising Strategy To Fine Regulate Bone ...
Xu S, Wang Y, Lu J, Xu J. Rheumatol Int. 2012 Nov;32(11):3397-403. Doi: 10.1007/S00296-011-2175-5. Epub 2011 Nov 6. Osteoprotegerin And Rankl In The Pathogenesis Of Rheumatoid Arthritis-Induced Osteoporosis.
Chen B, Li Xd, Liu Dx, Wang H, Xie P, Liu Zy, Hou Gq, Chang B, Du Sx. Phytomedicine. 2012 Aug 15;19(11):1029-34. Doi: 10.1016/J.Phymed.2012.06.002. Epub 2012 Jul 19. Canonical Wnt Signaling Is Required For Panax Notoginseng Saponin-Mediated Attenu...
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