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Product Info Summary
| SKU: | EK0642 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA) and human milk. |
Product info
Product Name
Human TSP2/THBS2/Thrombospondin-2 ELISA Kit PicoKine®
View all Thrombospondin-2 ELISA kits
SKU/Catalog Number
EK0642
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human TSP2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human THBS2 in cell culture supernatants, serum, plasma (heparin, EDTA) and human milk. Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human TSP2/THBS2/Thrombospondin-2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0642)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: G19-I1172
Sensitivity
<10 pg/ml
Assay Range
625 pg/ml - 40,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0642 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0642 is reactive to THBS2 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) and human milk.
Application Guarantee
EK0642 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Thrombospondin-2
Thrombospondin-2 (TSP-2) is a protein that in humans is encoded by the THBS2 gene.And this gene is mapped to 6q27. The protein encoded by this gene belongs to the thrombospondin family. It is a disulfide-linked homotrimeric glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein has been shown to function as a potent inhibitor of tumor growth and angiogenesis. Studies of the mouse counterpart suggest that this protein may modulate the cell surface properties of mesenchymal cells and be involved in cell adhesion and migration. TSP-2 has been shown to interact with MMP2.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0642-CAP | Anti-Human THBS2 Pre-coated 96-well strip microplate | 1 |
| EK0642-ST | Human THBS2 Standard | 2 vials, 40 ng/tube |
| EK0642-DA | Human THBS2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 | 40000 |
| O.D. | 0.025 | 0.110 | 0.191 | 0.343 | 0.605 | 0.977 | 1.630 | 2.067 |
Data Example Images
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Human TSP2 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Thrombospondin-2, Dilution ratio of 1:10, concentration in serum and plasma is around 50 ng/ml..
Some articles we found to cite concentrations of Thrombospondin-2 in samples: 25298262 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human TSP2/THBS2/Thrombospondin-2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 234 | 1702 | 6045 | 222 | 1773 | 6515 |
| Standard deviation | 17.55 | 789 | 411.06 | 17.76 | 117.54 | 501.65 |
| CV (%) | 7.5% | 4.6% | 6.8% | 8% | 6.5% | 7.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Thrombospondin-2 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 234 | 245 | 217 | 210 | 226 | 13.79 | 6.1% |
| Sample 2 | 1702 | 1693 | 1719 | 1783 | 1724 | 35.18 | 2% |
| Sample 3 | 6045 | 5875 | 6096 | 5562 | 5894 | 208.67 | 3.5% |
Protein Target Info & Infographic
Gene/Protein Information For THBS2 (Source: Uniprot.Org, NCBI)
Gene Name
THBS2
Full Name
Thrombospondin-2
Weight
129.991kDa
Superfamily
thrombospondin family
Alternative Names
THBS2; thrombospondin 2; Thrombospondin2; Thrombospondin-2; TSP-2; TSP2thrombospondin-2 THBS2 TSP2 thrombospondin 2 thrombospondin-2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on THBS2, check out the THBS2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for THBS2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human TSP2/THBS2/Thrombospondin-2 ELISA Kit PicoKine® (EK0642)
Hello CJ!
EK0642 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
miR-29a-3p/THBS2 Axis Regulates PAH-Induced Cardiac Fibrosis
Shi Y,Sun L,Zhang R,Hu Y,Wu Y,Dong X,Dong D,Chen C,Geng Z,Li E,Fan Y.Thrombospondin 4/integrin α2/HSF1 axis promotes proliferation and cancer stem-like traits of gallbladder cancer by enhancing reciprocal crosstalk between cancer-associated fibroblasts an
Species: Human,Mouse
EK0642 usage in article: APP:ELISA, SAMPLE:GBC CELL, DILUTION:NA
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
7 Customer Q&As for Human TSP2/THBS2/Thrombospondin-2 ELISA Kit PicoKine®
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of Thrombospondin-2 in Human plasma? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2020-06-23
Answer
A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Thrombospondin-2 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-06-23
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement Thrombospondin-2 using the EK0642 Human Thrombospondin-2 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-01-23
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2020-01-23
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
S. Krishna
Verified customer
Asked: 2019-11-13
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-11-13
Question
Q: is it okay to use citrate plasma as samples in Human Thrombospondin-2 Picokine® ELISA Kit (Catalog # EK0642)?
Verified Customer
Verified customer
Asked: 2019-08-02
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of Thrombospondin-2 causing degradation of its protein structure. Thrombospondin-2 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the Thrombospondin-2 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-08-02
Question
Q: What is the optimal O.D. value for Thrombospondin-2 ELISA kit? I performed your Thrombospondin-2 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Thrombospondin-2 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2017-08-28
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Thrombospondin-2 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Thrombospondin-2 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2017-08-28
Question
Q: Can Thrombospondin-2 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
C. Anand
Verified customer
Asked: 2016-12-08
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2016-12-08
Question
Q: how do I analyze ELISA data? I have obtained Thrombospondin-2 level in plasma.
J. Walker
Verified customer
Asked: 2016-02-05
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2016-02-05
