LIF Leukemia Inhibitory Factor Mouse Recombinant Protein

Product Name

LIF Leukemia Inhibitory Factor Mouse Recombinant Protein

View all LIF recombinant proteins

SKU/Catalog Number

PROTP09056

Size

3x2ug, 25ug, 1mg

Description

Leukemia Inhibitory Factor (LIF) Murine Recombinant produced in E. coli is a single, non-glycosylated, polypeptide chain containing 181 amino acids and having a molecular mass of 20 kDa. The Leukemia Inhibitory Factor (LIF) is purified by proprietary chromatographic techniques.

Storage & Handling

Lyophilized Leukemia Inhibitory Factor (LIF) although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Leukemia Inhibitory Factor (LIF) should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Cite This Product

LIF Leukemia Inhibitory Factor Mouse Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP09056)

Form

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Leukemia Inhibitory Factor (LIF) was lyophilized from a concentrated (1mg/ml) sterile solution containing 20mM Phosphate buffer pH-7.4 and 0.02% Tween-20.

Purity

Greater than 95.0% as determined by (a) Analysis by RP-HPLC and (b) Analysis by SDS-PAGE.

Predicted MW

22.008kDa

Reconstitution

It is recommended to reconstitute the lyophilized Leukemia Inhibitory Factor (LIF) in sterile water not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Biological Activity

Activity of murine LIF was determined by the M1 cell differentiation assay which was found to be less than 0.01 ng/ml, corresponding to a specific activity of 100,000,000 IU/mg. A standard of 50 Units is defined as the concentration of mouse LIF in 1.0 mL of tissue culture medium that induces the differentiation of 50% of M1 colonies.

Reconstitution

It is recommended to reconstitute the lyophilized Leukemia Inhibitory Factor (LIF) in sterile water not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Validation Images & Assay Conditions

Gene/Protein Information For LIF (Source: Uniprot.org, NCBI)

Alternative Names

CDF; HILDA; D-FACTOR; Differentiation- stimulating factor; Melanoma-derived LPL inhibitor; MLPLI; Emfilermin; Leukemia inhibitory factor; LIF; DIA LIF CDF, DIA, HILDA, MLPLI LIF interleukin 6 family cytokine leukemia inhibitory factor|D factor|cholinergic differentiation factor|differentiation inhibitory activity|differentiation-inducing factor|differentiation-stimulating factor|hepatocyte-stimulating factor III|human interleukin in DA cells|melanoma-derived LPL inhibitor

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

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