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Product Info Summary
| SKU: | EK0326 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA) and urine. |
Product info
Product Name
Mouse EGF ELISA Kit PicoKine®
SKU/Catalog Number
EK0326
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse EGF ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Egf in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 1pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse EGF ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0326)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: N977-R1029
Sensitivity
<1 pg/ml
Assay Range
7.8 pg/ml - 500 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0326 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0326 is reactive to EGF in Mouse samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) and urine.
Application Guarantee
EK0326 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of EGF
Epidermal growth factor or EGF is a growth factor that plays an important role in the regulation of cell growth, proliferation, and differentiation by binding to its receptor EGFR. EGF acts by binding with high affinity to epidermal growth factor receptor (EGFR) on the cell surface and stimulating the intrinsic protein-tyrosine kinase activity of the receptor. EGF results in cellular proliferation, differentiation, and survival. It also has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. EGF has strong expression in kidney, salivary gland, cerebrum, and prostate, moderate expression in trachea and thyroid, and low expression in bone marrow, heart, spleen, thymus, uterus, and colon. No expression was detected in adrenal gland, liver, lung, cerebellum, placenta, and small intestine.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0326-CAP | Anti-Mouse EGF Pre-coated 96-well strip microplate | 1 |
| EK0326-ST | Mouse EGF Standard | 2 vials, 10 ng/tube |
| EK0326-DA | Mouse EGF Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 7.8 | 15.6 | 31.3 | 62.5 | 125 | 250 | 500 |
| O.D. | 0.035 | 0.189 | 0.284 | 0.462 | 0.752 | 1.123 | 1.429 | 2.045 |
Data Example Images
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Mouse EGF PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect EGF, Dilution ratio of 1:1, concentration in serum and plasma is around 13 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse EGF ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 19 | 63 | 276 | 19 | 64 | 270 |
| Standard deviation | 1.42 | 4.34 | 17.38 | 1.53 | 5.44 | 18.63 |
| CV (%) | 7.5% | 6.9% | 8.5%% | 8.1% | 8.5% | 6.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of EGF in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 19 | 19 | 20 | 18 | 19 | 0.7 | 3.6% |
| Sample 2 | 63 | 76 | 72 | 72 | 70 | 4.76 | 6.8% |
| Sample 3 | 276 | 256 | 275 | 268 | 268 | 7.98 | 2.9% |
Protein Target Info & Infographic
Gene/Protein Information For EGF (Source: Uniprot.Org, NCBI)
Gene Name
EGF
Full Name
Pro-epidermal growth factor
Weight
133.994kDa
Alternative Names
beta-urogastrone; EGF; epidermal growth factor (beta-urogastrone); epidermal growth factor; HOMG4; pro-epidermal growth factor; URG; Urogastrone EGF HOMG4, URG epidermal growth factor pro-epidermal growth factor|beta-urogastrone
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on EGF, check out the EGF Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for EGF: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse EGF ELISA Kit PicoKine® (EK0326)
Hello CJ!
EK0326 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Cultured Cordyceps sinensis polysaccharides attenuate cyclophosphamide-induced intestinal barrier injury in mice
Russo F, Linsalata M, Clemente C, D'Attoma B, Orlando A, Campanella G, Giotta F, Riezzo G. Bmc Cancer. 2013 Feb 4;13:56. Doi: 10.1186/1471-2407-13-56. The Effects Of Fluorouracil, Epirubicin, And Cyclophosphamide (Fec60) On The Intestinal Barrier ...
Ge C, Yu M, Petitte Jn, Zhang C. Biol Reprod. 2009 Mar;80(3):528-36. Doi: 10.1095/Biolreprod.108.072728. Epub 2008 Nov 12. Epidermal Growth Factor-Induced Proliferation Of Chicken Primordial Germ Cells: Involvement Of Calcium/Protein Kinase C And ...
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Mouse EGF ELISA Kit PicoKine®
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis EGF using the EK0326 Mouse EGF Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-10-19
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2018-10-19
Question
Q: can you suggest the dilution ratio of serum samples for detection of EGF in Mouse serum? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2018-07-07
Answer
A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the EGF ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-07-07
Question
Q: can I use citrate plasma as samples in Mouse EGF Picokine® ELISA Kit (Catalog # EK0326)?
Verified Customer
Verified customer
Asked: 2016-09-21
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of EGF causing disruption of its protein structure. EGF may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the EGF ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2016-09-21
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2016-03-04
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2016-03-04
Question
Q: how to proceed with the analysis of ELISA data? I measured EGF level in serum.
Verified Customer
Verified customer
Asked: 2015-04-19
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2015-04-19
