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Product Info Summary
|Size:||For 5 plates, 96 wells each|
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Rat EGF ELISA Kit EZ-Set™ (DIY Antibody Pairs)
For 5 plates, 96 wells each
*Question: How many samples can I assay/run in this kit?
Rat EGF ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Rat EGF in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 1 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat EGF ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ0954)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: E.coli; Immunogen sequence: N974-R1026
7.8 pg/ml - 500 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EZ0954 is reactive to EGF in Rat samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) and urine.
EZ0954 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EZ0954-CA||Rabbit anti- rat Egf polyclonal antibody (Capture Antibody)||500 µl, 0.4 mg/mL (recommended dilution 1:100)||0.5% Na2HPO4, 0.04% Proclin 300 and 50% Glycerol|
|EZ0594-DA||Biotinylated goat anti- rat Egf polyclonal antibody (Detection Antibody)||500 µl, 2.5 µg/mL (recommended dilution 1:100)||0.04% Proclin 300, 0.5% BSA, 0.2% Tris, 1% NaCl and 30% Glycerol|
|EZ0594-ST||Lyophilized recombinant rat Egf standard||10 ng/tube×2|
|AR1103||Avidin-Biotin-Peroxidase Complex (ABC)||500 µl (recommended dilution 1:100)|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- 1. Microplate reader in standard size.
- 2. Automated plate washer.
- 3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- 4. Clean tubes and Eppendorf tubes.
- 5. 96 well microplate (Cat# AR1100)
- 6. Plate Sealers.
- 7. Capture Antibody Diluent: PBS.
- 8. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
- 9. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
- 10. Stop Solution: 2 N H2SO4 (Cat# AR1105)
- 11. Wash Buffer (PBS and PBS-T). Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
- *Item 5 – 11 are included in the EZ Set Accessory Kit (EZA001)
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Rat EGF EZ-Set ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
Gene/Protein Information For EGF (Source: Uniprot.Org, NCBI)
Pro-epidermal growth factor
beta-urogastrone; EGF; epidermal growth factor (beta-urogastrone); epidermal growth factor; HOMG4; pro-epidermal growth factor; URG; Urogastrone Egf|epidermal growth factor|pro-epidermal growth factor*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on EGF, check out the EGF Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for EGF: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for EZ0954
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