Mouse IL-10 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Mouse IL-10 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Mouse IL10 in cell culture supernatants, serum. Sensitivity: 1 pg/ml. Cited in 6 publication(s).

Product Info Summary

SKU: EZ0417
Size: For 5 plates, 96 wells each
Reactive Species: Mouse
Application: ELISA

Product Name

Mouse IL-10 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

See all IL-10 products

SKU/Catalog Number

EZ0417

Size

For 5 plates, 96 wells each

*Question: How many samples can I assay/run in this kit?

Description

Mouse IL-10 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Mouse IL10 in cell culture supernatants, serum. Sensitivity: 1 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse IL-10 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ0417)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S19-N178

Sensitivity

<1 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EZ0417 is reactive to IL10 in Mouse samples

Validated Sample Types

cell culture supernatants, serum.

Application Guarantee

EZ0417 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog number Description Quantity Buffers
EZ0417-CA Rabbit anti- mouse Il10 polyclonal antibody (Capture Antibody) 500 µl, 0.4 mg/mL (recommended dilution 1:100) 0.5% Na2HPO4, 0.04% Proclin 300 and 50% Glycerol
EZ0417-DA Biotinylated goat anti- mouse Il10 polyclonal antibody (Detection Antibody) 500 µl, 14 µg/mL (recommended dilution 1:100) 0.04% Proclin 300, 0.5% BSA, 0.2% Tris, 1% NaCl and 30% Glycerol
EZ0417-ST Lyophilized recombinant mouse Il10 standard 10 ng/tube×2
AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 µl (recommended dilution 1:100)

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • 1. Microplate reader in standard size.
  • 2. Automated plate washer.
  • 3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • 4. Clean tubes and Eppendorf tubes.
  • 5. 96 well microplate (Cat# AR1100)
  • 6. Plate Sealers.
  • 7. Capture Antibody Diluent: PBS.
  • 8. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
  • 9. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
  • 10. Stop Solution: 2 N H2SO4 (Cat# AR1105)
  • 11. Wash Buffer (PBS and PBS-T). Recipes:
    PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
    PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
  • *Item 5 – 11 are included in the EZ Set Accessory Kit (EZA001)

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0550.1290.1840.3220.5070.9431.2941.905

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3321946635203420
Standard deviation1.8814.0119.12.6616.6425.2
CV (%)5.7%6.4%4.1%7.6%8.2%6%

Gene/Protein Information For IL10 (Source: Uniprot.Org, NCBI)

Uniprot ID

P18893

Gene ID

16153

Gene Name

IL10

Full Name

Interleukin-10

Weight

20.641kDa

Superfamily

IL-10 family

Alternative Names

CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF Il10|CSIF, IL-, If2a, Il-10|interleukin 10|interleukin-10|cytokine synthesis inhibitory factor|interferon 2a

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on IL10, check out the IL10 Infographic

IL10 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for IL10: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EZ0417 has been cited in 6 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

TLR4 signaling pathway in mouse Lewis lung cancer cells promotes the expression of TGF-β1 and IL-10 and tumor cells migration

MiR-137 attenuates spinal cord injury by modulating NEUROD4 through reducing inflammation and oxidative stress

Immune Protection of Rhoptry Protein 21 (ROP21) of Toxoplasma gondii as a DNA Vaccine Against Toxoplasmosis

Immunological adjuvant effect of the peptide fraction from the larvae of Musca domestica

Mmu-miR-27a-5p-Dependent Upregulation of MCPIP1 Inhibits the Inflammatory Response in LPS-Induced RAW264.7 Macrophage Cells

Lu C, Liu H, Jin X, Chen Y, Liang CL, Qiu F, Dai Z. Front Pharmacol. 2018 Feb 12;9:88. doi: 10.3389/fphar.2018.00088. eCollection 2018. Herbal Components of a Novel Formula PSORI-CM02 Interdependently Suppress Allograft Rejection and Induce CD8+CD...

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2 Customer Q&As for Mouse IL-10 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Question

What is the clone number of EZ0417 antibody that the plates are coated with?

Verified Customer

Verified customer

Asked: 2020-01-17

Answer

The EZ0417 antibody is a goat polyclonal as capture antibody, and a rat monoclonal [B-C40] for detection.

Boster Scientific Support

Answered: 2020-01-17

Question

I would like to ask if this Mouse IL-10 ELISA Kit EZ-Set

Verified Customer

Verified customer

Asked: 2018-06-25

Answer

The antibody Mouse IL-10 ELISA Kit EZ-Set

Boster Scientific Support

Answered: 2018-06-25

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