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Product Info Summary
| SKU: | EK0417 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants and serum. |
Product info
Product Name
Mouse IL-10/Interleukin-10 ELISA Kit PicoKine®
SKU/Catalog Number
EK0417
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse IL-10/Interleukin-10 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Il10 in cell culture supernatants and serum. Sensitivity: 1pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse IL-10/Interleukin-10 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0417)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: S19-S178
Sensitivity
<1 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0417 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0417 is reactive to IL10 in Mouse samples
Validated Sample Types
cell culture supernatants and serum.
Application Guarantee
EK0417 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IL-10
Interleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis, and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production. IL-10 is an important cytokine with anti-inflammatory, anti-immune, and antifibrotic functions. It is also an important regulatory cytokine whose involvement extends into diverse areas of the human immune system. IL-10 is a recently described natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8. Interleukin-10 gene polymorphisms and interleukin-10 production capability may contribute to the development of skin squamous cell carcinomas after renal transplantation. The interleukin-10 locus contributes to the heritability of psoriasis susceptibility. With regard to sudden infant death, IL-10 is of special interest. This is an immunoregulatory cytokine that plays an important role in the development of infectious disease. The mIL-10 gene is mapped to mouse chromosome 1 and the hIL-10 gene is also mapped to human chromosome 1. The standard product used in this kit is recombinant mouse IL-10, consisting of 160 amino acid with the molecular mass of 18KDa.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0417-CAP | Anti-Mouse IL10 Pre-coated 96-well strip microplate | 1 |
| EK0417-ST | Mouse IL10 Standard | 2 vials, 10 ng/tube |
| EK0417-DA | Mouse IL10 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.051 | 0.095 | 0.165 | 0.267 | 0.450 | 0.758 | 1.278 | 1.840 |
Data Example Images
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Mouse IL-10 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect IL-10, Dilution ratio of 1:1, concentration in serum is less than the lowest standard, 15.6 pg/ml..
Some articles we found to cite concentrations of IL-10 in samples: 24809259 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse IL-10/Interleukin-10 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 33 | 219 | 466 | 35 | 203 | 420 |
| Standard deviation | 1.88 | 14.01 | 19.1 | 2.66 | 16.64 | 25.7 |
| CV (%) | 5.7% | 6.4% | 4.1% | 7.6% | 8.2% | 6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of IL-10 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 33 | 38 | 38 | 37 | 36 | 2.06 | 5.7% |
| Sample 2 | 219 | 193 | 202 | 190 | 201 | 11.29 | 5.6% |
| Sample 3 | 466 | 448 | 405 | 427 | 436 | 22.83 | 5.2% |
Protein Target Info & Infographic
Gene/Protein Information For IL10 (Source: Uniprot.Org, NCBI)
Gene Name
IL10
Full Name
Interleukin-10
Weight
20.517kDa
Superfamily
IL-10 family
Alternative Names
CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF IL10 CSIF, GVHDS, IL-10A, TGIF, IL10 interleukin 10 interleukin-10|T-cell growth inhibitory factor|cytokine synthesis inhibitory factor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on IL10, check out the IL10 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IL10: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse IL-10/Interleukin-10 ELISA Kit PicoKine® (EK0417)
Hello CJ!
EK0417 has been cited in 266 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Chronic stress promoted the growth of ovarian carcinoma via increasing serum levels of norepinephrine and interleukin-10 and altering nm23 and NDRG1 expression in tumor tissues in nude mice
Hybrid phage displaying SLAQVKYTSASSI induces protection against Candida albicans challenge in BALB/c mice
Tumor cell lysate induces the immunosuppression and apoptosis of mouse immunocytes
Decitabine attenuates dextran sodium sulfate‑induced ulcerative colitis through regulation of immune regulatory cells and intestinal barrier
LncRNA uc.48+ is involved in the diabetic immune and inflammatory responses mediated by P2X7 receptor in RAW264.7 macrophages
Resveratrol protects against L-arginine-induced acute necrotizing pancreatitis in mice by enhancing SIRT1-mediated deacetylation of p53 and heat shock factor 1
Programmed cell death‑1/programmed cell death‑ligand 1 inhibitors exert antiapoptosis and antiinflammatory activity in lipopolysaccharide stimulated murine alveolar macrophages
lncRNA uc.48+ regulates immune and inflammatory reactions mediated by the P2X7 receptor in type 2 diabetic mice
Identification of Toxoplasma Gondii Tyrosine Hydroxylase (TH) Activity and Molecular Immunoprotection against Toxoplasmosis
β-Glucan Extracted from Highland Barley Alleviates Dextran Sulfate Sodium-Induced Ulcerative Colitis in C57BL/6J Mice
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
6 Customer Q&As for Mouse IL-10/Interleukin-10 ELISA Kit PicoKine®
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring IL-10 using the EK0417 Mouse IL-10 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-07-05
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2020-07-05
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2019-10-27
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-10-27
Question
Q: if the enzyme conjugated IL-10 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-10 antigen?
Verified Customer
Verified customer
Asked: 2019-10-25
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-10-25
Question
Dear Support: Just want to know what is the clone number of the Mouse IL-10 ELISA Kit I purchased, that the plates are coated with?
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
The antibodies used in Mouse IL-10 ELISA Kit are goat polyclonal as capture antibody, and rat monoclonal [B-C40] for detection.
Boster Scientific Support
Answered: 2019-06-26
Question
Q: can you suggest the dilution ratio of serum samples for detection of IL-10 in Mouse serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2018-07-25
Answer
A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the IL-10 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-07-25
Question
I purchased Mouse IL-10 ELISA Kit PicoKine, I just want to make sure if this kit works on mouse blood sample.
Verified Customer
Verified customer
Asked: 2015-02-16
Answer
The product Mouse IL-10 ELISA Kit PicoKine have been tested and validated in mouse plasma and serum, however we would not recommend to use it in whole blood.
Boster Scientific Support
Answered: 2015-02-16
