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Product Info Summary
| SKU: | EK0880 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA) and urine. |
Product info
Product Name
Mouse KIM1 / TIM1 ELISA Kit PicoKine®
View all TIM-1/KIM-1/HAVCR ELISA kits
SKU/Catalog Number
EK0880
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse KIM1 / TIM1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Havcr1 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 2pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse KIM1 / TIM1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0880)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: Y22-T212
Sensitivity
<2 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0880 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0880 is reactive to HAVCR1 in Mouse samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) and urine.
Application Guarantee
EK0880 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of TIM-1/KIM-1/HAVCR
KIM1 (TIM-1), also known as Hepatitis A virus cellular receptor 1, is a protein that in Mouses is encoded by the HAVCR1 gene.[1][2][3] Infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV led Feigelstock et al. (1998) to conclude that the protein is indeed a receptor for the virus. Immunofluorescence microscopy demonstrated internalization of HAV by dog cells expressing HAVCR1. Using a monoclonal antibody to mouse Tim1, Umetsu et al. (2005) showed that Tim1 was expressed after activation of naive T cells and on T cells differentiated in Th2-polarizing conditions. By homology of synteny with the mouse Tim1 gene and database analysis, McIntire et al. (2001) mapped the HAVCR1 gene to 5q33.2. The standard used in this kit is recombinant mouse KIM-1, constituting from Y22-T212 amino acids, and 191amino acids with the molecular mass of 21.8KDa.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0880-CAP | Anti-Mouse HAVCR1 Pre-coated 96-well strip microplate | 1 |
| EK0880-ST | Mouse HAVCR1 Standard | 2 vials, 10 ng/tube |
| EK0880-DA | Mouse HAVCR1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.036 | 0.061 | 0.104 | 0.172 | 0.294 | 0.520 | 0.977 | 1.756 |
Data Example Images
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Mouse KIM1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect TIM-1/KIM-1/HAVCR, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 31.2 pg/ml..
Some articles we found to cite concentrations of TIM-1/KIM-1/HAVCR in samples: 23019274 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse KIM1 / TIM1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 87 | 279 | 842 | 85 | 261 | 792 |
| Standard deviation | 6.87 | 14.78 | 36.2 | 7.31 | 14.87 | 38.8 |
| CV (%) | 7.9% | 5.3% | 4.3% | 8.6% | 5.7% | 4.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of TIM-1/KIM-1/HAVCR in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 87 | 90 | 90 | 96 | 90 | 3.26 | 3.6% |
| Sample 2 | 279 | 266 | 249 | 284 | 269 | 13.53 | 5% |
| Sample 3 | 842 | 734 | 811 | 767 | 788 | 41.23 | 5.2% |
Protein Target Info & Infographic
Gene/Protein Information For HAVCR1 (Source: Uniprot.Org, NCBI)
Gene Name
HAVCR1
Full Name
Hepatitis A virus cellular receptor 1
Weight
39.25kDa
Superfamily
immunoglobulin superfamily
Alternative Names
CD365; HAVCR1; HAVCR-1; HAVCRT cell immunoglobin domain and mucin domain protein 1; hepatitis A virus cellular receptor 1; Kidney injury molecule 1; KIM1; KIM-1; T-cell immunoglobulin and mucin domain-containing protein 1; TIM1; TIM-1; TIM-1TIM; TIM1TIMD-1; TIMD1T-cell membrane protein 1 HAVCR1 CD365, HAVCR, HAVCR-1, KIM-1, KIM1, TIM, TIM-1, TIM1, TIMD-1, TIMD1 hepatitis A virus cellular receptor 1 hepatitis A virus cellular receptor 1|T cell immunoglobin domain and mucin domain protein 1|T-cell immunoglobulin mucin family member 1|T-cell immunoglobulin mucin receptor 1|T-cell membrane protein 1|kidney injury molecule 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on HAVCR1, check out the HAVCR1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for HAVCR1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse KIM1 / TIM1 ELISA Kit PicoKine® (EK0880)
Hello CJ!
EK0880 has been cited in 6 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Hydrogen sulfide attenuates renal I/R‑induced activation of the inflammatory response and apoptosis via regulating Nrf2‑mediated NLRP3 signaling pathway inhibition
Inhibition of aerobic glycolysis alleviates sepsis‑induced acute kidney injury by promoting lactate/Sirtuin 3/AMPK‑regulated autophagy
Activated iRhom2 drives prolonged PM2.5 exposure-triggered renal injury in Nrf2-defective mice
Li H,Sun H,Xu Y,Xing G,Wang X.Curcumin plays a protective role against septic acute kidney injury by regulating the TLR9 signaling pathway.Transl Androl Urol.2021 May;10(5):2103-2112.doi:10.21037/tau-21-385.PMID:34159091;PMCID:PMC8185681.
Species: Rat
EK0880 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Su Y,Wang Y,Liu M,Chen H.Hydrogen sulfide attenuates renal I/R‑induced activation of the inflammatory response and apoptosis via regulating Nrf2‑mediated NLRP3 signaling pathway inhibition.Mol Med Rep.2021 Jul;24(1):518.doi:10.3892/mmr.2021.12157.Epub 2021 May 20.PMID:34013370.
Species: Mouse
EK0880 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Tan C,Gu J,Li T,Chen H,Liu K,Liu M,Zhang H,Xiao X.Inhibition of aerobic glycolysis alleviates sepsis‑induced acute kidney injury by promoting lactate/Sirtuin 3/AMPK‑regulated autophagy.Int J Mol Med.2021 Mar;47(3):19.doi:10.3892/ijmm.2021.4852.Epub 2021 J
Species: Human,Mouse
EK0880 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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12 Customer Q&As for Mouse KIM1 / TIM1 ELISA Kit PicoKine®
Question
Does EK0880 contain material sourced from human origin?
Verified customer
Asked: 2022-03-31
Answer
No, the Mouse KIM1 / TIM1 ELISA Kit PicoKine® (EK0880) does not contain material sourced from human origin.
Boster Scientific Support
Answered: 2022-04-01
Question
Does EK0880 contain viable microorganism?
Verified customer
Asked: 2022-03-31
Answer
No, the Mouse KIM1 / TIM1 ELISA Kit PicoKine® (EK0880) does not contain viable microorganism.
Boster Scientific Support
Answered: 2022-04-01
Question
Does EK0880 contain genetically modified organisms?
Verified customer
Asked: 2022-03-31
Answer
No, the Mouse KIM1 / TIM1 ELISA Kit PicoKine® (EK0880) does not contain genetically modified organisms.
Boster Scientific Support
Answered: 2022-04-01
Question
Does EK0880 contain animal derived material?
Verified customer
Asked: 2022-03-31
Answer
Yes, the Mouse KIM1 / TIM1 ELISA Kit PicoKine® (EK0880) does not contain animal derived material. - If it does (for each!): Species: Goat (capture and detection antibodies) Bovine(Standard, Sample Diluent, Antibody Diluent and Avidin-Biotin-Peroxidase Diluent contain BSA) - If it does (for each!): Country of Origin: Goat (The USA) Bovine (New Zealand)
Boster Scientific Support
Answered: 2022-04-01
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
K. Rodriguez
Verified customer
Asked: 2020-08-21
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-08-21
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2020-01-05
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-01-05
Question
Q: how can I thaw whole blood sample for TIM-1 ELISA after freezing?
A. Sunder
Verified customer
Asked: 2019-10-28
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test TIM-1 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2019-10-28
Question
Q: can I use citrate plasma as samples in Mouse TIM-1 Picokine® ELISA Kit (Catalog # EK0880)?
P. Bhatt
Verified customer
Asked: 2019-04-02
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of TIM-1 causing degradation of its protein structure. TIM-1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the TIM-1 ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-04-02
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2019-01-11
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-01-11
Question
Q: can you suggest the dilution ratio of serum samples for detection of TIM-1 in Mouse urine? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2018-07-09
Answer
A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the TIM-1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-07-09
Question
Q: if the enzyme conjugated TIM-1 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the TIM-1 antigen?
Verified Customer
Verified customer
Asked: 2018-01-30
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-01-30
Question
Q: how to analyze ELISA data? I have obtained TIM-1 level in urine.
W. Davis
Verified customer
Asked: 2016-01-01
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2016-01-01
