Product Info Summary
SKU: | EK1697 |
---|---|
Size: | 96 wells/kit, with removable strips. |
Reactive Species: | Mouse |
Application: | ELISA |
Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse PDGF-BB ELISA Kit PicoKine®
SKU/Catalog Number
EK1697
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse PDGF-BB ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse PDGF-BB in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml .
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse PDGF-BB ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1697)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: S82-T190
Sensitivity
<10 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1697 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1697 is reactive to Pdgfb in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK1697 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Pdgfb
PDGF-BB encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.
Kit Components
Catalog Number | Description | Quantity |
---|---|---|
EK1697-CAP | Anti-Mouse Pdgfb Pre-coated 96-well strip microplate | 1 |
EK1697-ST | Mouse Pdgfb Standard | 2 vials, 10 ng/tube |
EK1697-DA | Mouse Pdgfb Biotinylated antibody (100x) | 100ul |
AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
AR1106-1 | Sample Diluent | 30ml |
AR1106-2 | Antibody Diluent | 12ml |
AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop Solution | 10ml |
AR1106-5 | Wash Buffer (25x) | 20ml |
PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.066 | 0.045 | 0.129 | 0.198 | 0.478 | 0.991 | 1.817 | 2.424 |
Data Example Images
Click image to see more details
Mouse PDGF-BB PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Pdgfb, For serum, dilution ratio is 1:20 and concentration is 8216-20868 pg/ml.
For plasma, dilution ratio is 1:5 and concentration is 280-9852 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse PDGF-BB ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 149 | 683 | 1203 | 150 | 750 | 1331 |
Standard deviation | 6.7 | 48.49 | 56.54 | 7.95 | 43.5 | 82.5 |
CV (%) | 4.5% | 7.1% | 4.7% | 5.3% | 5.8% | 6.2% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Pdgfb in ELISA kits from four different production batches/lots.
Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
---|---|---|---|---|---|---|---|
Sample 1 | 149 | 172 | 178 | 169 | 167 | 10.88 | 6.5% |
Sample . 2 | 683 | 696 | 665 | 663 | 676 | 13.57 | 2.0%% |
Sample 3 | 1203 | 1369 | 1343 | 1427 | 1335 | 82.32 | 6.1% |
Protein Target Info & Infographic
Gene/Protein Information For Pdgfb (Source: Uniprot.Org, NCBI)
Gene Name
Pdgfb
Full Name
Platelet-derived growth factor subunit B
Weight
27.382kDa
Superfamily
PDGF/VEGF growth factor family
Alternative Names
PDGFBB; PDGF-BB Pdgfb|PDGF-, PDGF-2, PDGF-B, Sis, c-sis|platelet derived growth factor, B polypeptide|platelet-derived growth factor subunit B|PDGF subunit B|platelet-derived growth factor B chain|platelet-derived growth factor beta polypeptide|proto-oncogene c-Sis
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Pdgfb, check out the Pdgfb Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Pdgfb: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse PDGF-BB ELISA Kit PicoKine® (EK1697)
Hello CJ!
EK1697 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Liu Y,Feng M,Cai J,Li S,Dai X,Shan G,Wu S.Repurposing bortezomib for choroidal neovascularization treatment via antagonizing VEGF-A and PDGF-D mediated signaling.Exp Eye Res.2021 Jan 18;204:108446.doi:10.1016/j.exer.2021.108446.Epub ahead of print.PMID:33
Species: Mouse
EK1697 usage in article: APP:ELISA, SAMPLE:RPE TISSUE, DILUTION:NA
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Customer Reviews
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Customer Q&As
Have a question?
Find answers in Q&As, reviews.
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Submit your question
9 Customer Q&As for Mouse PDGF-BB ELISA Kit PicoKine®
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2019-05-27
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-05-27
Question
Q: how can I thaw whole blood sample for PDGFB ELISA after freezing?
Verified Customer
Verified customer
Asked: 2018-08-08
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test PDGFB for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2018-08-08
Question
Q: how do I analyze ELISA data? I measured PDGFB level in plasma.
S. Gonzalez
Verified customer
Asked: 2018-08-03
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-08-03
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring PDGFB using the EK1697 Mouse PDGFB Picokine® ELISA Kit?
Y. Martin
Verified customer
Asked: 2018-07-04
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2018-07-04
Question
Q: is it okay to use citrate plasma as samples in Mouse PDGFB Picokine® ELISA Kit (Catalog # EK1697)?
Verified Customer
Verified customer
Asked: 2018-06-22
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of PDGFB causing disruption of its protein structure. PDGFB may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the PDGFB ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-06-22
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
H. Wang
Verified customer
Asked: 2017-10-05
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2017-10-05
Question
Q: What is the optimal O.D. value for PDGFB ELISA kit? I performed your PDGFB ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain PDGFB even though the O.D. values are not very high?
M. Martin
Verified customer
Asked: 2017-09-19
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this PDGFB ELISA kit to have sensitivity of 10pg/ml , that means the minimum amount of PDGFB that can be declared/interpreted as positive by the above standard is 10pg/ml .
Boster Scientific Support
Answered: 2017-09-19
Question
Q: Can PDGFB ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2016-11-24
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2016-11-24
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
S. Krishna
Verified customer
Asked: 2015-07-05
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2015-07-05