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Product Info Summary
| SKU: | EK1618 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse PGLYRP1/Pgrps ELISA Kit PicoKine®
View all PGLYRP1/PGRP-S ELISA kits
SKU/Catalog Number
EK1618
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse PGLYRP1/Pgrps ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Pglyrp1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse PGLYRP1/Pgrps ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1618)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: F19-E182
Sensitivity
<10 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1618 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1618 is reactive to PGLYRP1 in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK1618 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of PGLYRP1/PGRP-S
Peptidoglycan recognition protein 1, also known as TAG7, is a protein that in humans is encoded by the PGLYRP1 gene. The PGLYRP1 gene is highly expressed in bone marrow, circulating Polymorphonuclear leukocytes (PMLs), and in the corneal epithelium. The PGLYRP1 protein is primarily found in the granules of PMLs. The PGLYRP1 protein plays an important role in the innate immune response. It is bactericidal against gram-positive bacteria such as S. aureus, S. epidermidis, and L. monocytogenes and generally has proinflammatory effects, inducing TNF-a and IFN-g in many tissues. PGLYRP-1 is also known to form a cytotoxic complex with HSP-70, suggesting it may also have a role in anti-cancer defense. As a pathogen recognition protein with antimicrobial properties, PGLYRP-1 is suspected to play an important role in maintaining the gut microbial flora.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1618-CAP | Anti-Mouse PGLYRP1 Pre-coated 96-well strip microplate | 1 |
| EK1618-ST | Mouse PGLYRP1 Standard | 2 vials, 10 ng/tube |
| EK1618-DA | Mouse PGLYRP1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.024 | 0.105 | 0.157 | 0.288 | 0.541 | 0.848 | 1.329 | 1.819 |
Data Example Images
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Mouse PGLYRP1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect PGLYRP1/PGRP-S, Dilution ratio of 1:200, concentration in serum and plasma is 33-100 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse PGLYRP1/Pgrps ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 34 | 223 | 498 | 37 | 211 | 460 |
| Standard deviation | 1.87 | 16.05 | 27.39 | 2.03 | 16.24 | 26.68 |
| CV (%) | 5.5% | 7.2% | 5.5% | 5.5% | 7.7% | 5.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of PGLYRP1/PGRP-S in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 34 | 31 | 32 | 32 | 32 | 1.08 | 3.3% |
| Sample 2 | 223 | 229 | 202 | 199 | 213 | 12.96 | 6% |
| Sample 3 | 498 | 462 | 442 | 494 | 474 | 23.15 | 4.8% |
Protein Target Info & Infographic
Gene/Protein Information For PGLYRP1 (Source: Uniprot.Org, NCBI)
Gene Name
PGLYRP1
Full Name
Peptidoglycan recognition protein 1
Weight
21.731kDa
Superfamily
N-acetylmuramoyl-L-alanine amidase 2 family
Alternative Names
peptidoglycan recognition protein 1; Peptidoglycan recognition protein short; PGLYRP; PGLYRP1; PGRPpeptidoglycan recognition protein; PGRPS; PGRP-S; PGRP-SMGC126894; PGRPSMGC126896; TAG7; TNF superfamily, member 3 (LTB)-like (peptidoglycan recognition protein); TNFSF3L PGLYRP1 PGLYRP, PGRP, PGRP-S, PGRPS, TAG7, TNFSF3L peptidoglycan recognition protein 1 peptidoglycan recognition protein 1|TNF superfamily, member 3 (LTB)-like (peptidoglycan recognition protein)
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on PGLYRP1, check out the PGLYRP1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for PGLYRP1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse PGLYRP1/Pgrps ELISA Kit PicoKine® (EK1618)
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No publications found for EK1618
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Customer Reviews
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Customer Q&As
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9 Customer Q&As for Mouse PGLYRP1/Pgrps ELISA Kit PicoKine®
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-04-21
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2020-04-04
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-04-04
Question
Q: how to proceed with the analysis of ELISA data? I have performed PGLYRP1 level in plasma.
Verified Customer
Verified customer
Asked: 2019-07-24
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-07-24
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis PGLYRP1 using the EK1618 Mouse PGLYRP1 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-02-25
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2019-02-25
Question
Q: if the enzyme conjugated PGLYRP1 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the PGLYRP1 antigen?
S. Campbell
Verified customer
Asked: 2018-11-19
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-11-19
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of PGLYRP1 in Mouse plasma? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2018-11-07
Answer
A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the PGLYRP1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-11-07
Question
Q: Can PGLYRP1 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
K. Parker
Verified customer
Asked: 2018-09-29
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-09-29
Question
Q: can I use citrate plasma as samples in Mouse PGLYRP1 Picokine® ELISA Kit (Catalog # EK1618)?
H. Thompson
Verified customer
Asked: 2017-09-21
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of PGLYRP1 causing degradation of its protein structure. PGLYRP1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the PGLYRP1 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2017-09-21
Question
Q: how can I thaw whole blood sample for PGLYRP1 ELISA after freezing?
W. Garcia
Verified customer
Asked: 2015-08-20
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test PGLYRP1 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2015-08-20
