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Product Info Summary
| SKU: | EK0582 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin). |
Product info
Product Name
Mouse Resistin ELISA Kit PicoKine®
SKU/Catalog Number
EK0582
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse Resistin ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Retn in cell culture supernatants, serum and plasma (heparin). . Sensitivity: 2pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse Resistin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0582)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: S21-S114
Sensitivity
<2 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0582 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0582 is reactive to RETN in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin).
Application Guarantee
EK0582 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Resistin
Resistin is an adipose-derived hormone postulated to link adiposity to insulin resistance. Type 2 diabetes, characterized by target-tissue resistance to insulin, is epidemic in industrialized societies and is strongly associated with obesity. Adipocytes secrete a unique signalling molecule, which called resistin (for resistance to insulin), that may be the hormone potentially links obesity to diabetes.Resistin is overexpressed in mouse adipose tissue of obese individuals and is likely to modulate insulin sensitivity. Resistin is, therefore, a candidate gene for insulin resistance.The standard product used in this kit is mouse Resistin with the molecular mass of 51-53KDa. The detected Resistin includes zymogen and active enzyme.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0582-CAP | Anti-Mouse RETN Pre-coated 96-well strip microplate | 1 |
| EK0582-ST | Mouse RETN Standard | 2 vials, 10 ng/tube |
| EK0582-DA | Mouse RETN Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.25 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | |
| O.D. | 0.006 | 0.122 | 0.245 | 0.441 | 0.743 | 1.101 | 1.748 | 2.241 |
Data Example Images
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Mouse Resistin PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Resistin, Dilution ratio of 1:100, concentration in serum and plasma is 14-100 ng/ml..
Some articles we found to cite concentrations of Resistin in samples: 24266722 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse Resistin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 77 | 245 | 929 | 80 | 252 | 861 |
| Standard deviation | 6.2%2 | 13.23 | 57.59 | 7.28 | 17.64 | 69.74 |
| CV (%) | 7.7% | 5.4% | 6.2% | 9.1% | 7% | 8.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Resistin in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 77 | 78 | 88 | 84 | 81 | 4.49 | 5.5% |
| Sample 2 | 245 | 263 | 267 | 252 | 256 | 8.72 | 3.4% |
| Sample 3 | 929 | 803 | 900 | 906 | 884 | 48.28 | 5.4% |
Protein Target Info & Infographic
Gene/Protein Information For RETN (Source: Uniprot.Org, NCBI)
Gene Name
RETN
Full Name
Resistin
Weight
11.419kDa
Superfamily
resistin/FIZZ family
Alternative Names
Adipose tissue-specific secretory factor; ADSF; ADSFMGC126609; C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich proteinprecursor 1; C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; Cysteine-rich secreted protein A12-alpha-like 2; Cysteine-rich secreted protein FIZZ3; FIZZ3; FIZZ3MGC126603; found in inflammatory zone 3; HXCP1; Resistin; RETN; RETN1; RSTNXCP1 RETN ADSF, FIZZ31, RSTN, XCP1, RETN resistin resistin|C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich protein precursor 1|adipose tissue-specific secretory factor|c/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein|cysteine-rich secreted protein A12-alpha-like 2|cysteine-rich secreted protein FIZZ3|found in inflammatory zone 3|resistin delta2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on RETN, check out the RETN Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for RETN: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse Resistin ELISA Kit PicoKine® (EK0582)
Hello CJ!
EK0582 has been cited in 7 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
NAD+-boosting therapy alleviates nonalcoholic fatty liver disease via stimulating a novel exerkine Fndc5/irisin
Effects of replacing wheat flour with detoxified ginkgo nut powder on lipid metabolism of obese C57BL/6J male mice
Consumption of phenolic-rich jabuticaba (Myrciaria jaboticaba) powder ameliorates obesity-related disorders in mice
Anti-inflammatory and antioxidant effects of polyphenols extracted from Antirhea borbonica medicinal plant on adipocytes exposed to Porphyromonas gingivalis and Escherichia coli lipopolysaccharides
Porphyromonas gingivalis lipopolysaccharide induces pro-inflammatory adipokine secretion and oxidative stress by regulating Toll-like receptor-mediated signaling pathways and redox enzymes in adipocytes
The Beneficial Effect of Anthocyanidin‐Rich Vitis vinifera L. Grape Skin Extract on Metabolic Changes Induced by High‐Fat Diet in Mice Involves Antiinflammatory and Antioxidant Actions
Trindade PL,Soares EDR,Inada KOP,Martins FF,Rudnicki M,Perrone D,Monteiro M,Souza-Mello V,Daleprane JB.Consumption of phenolic-rich jabuticaba (Myrciaria jaboticaba) powder ameliorates obesity-related disorders in mice.Br J Nutr.2021 Mar 31:1-30.doi:10.1017/S0007114521001136.Epub ahead of print.PMID:33787474.
Species: Mouse
EK0582 usage in article: APP:ELISA, SAMPLE:BLOOD, DILUTION:NA
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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8 Customer Q&As for Mouse Resistin ELISA Kit PicoKine®
Question
Q: how to thaw whole blood sample for Resistin ELISA after freezing?
Verified Customer
Verified customer
Asked: 2020-12-04
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Resistin for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2020-12-04
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2019-12-10
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-12-10
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
R. Campbell
Verified customer
Asked: 2019-11-04
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-11-04
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement Resistin using the EK0582 Mouse Resistin Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-05-13
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2019-05-13
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2019-04-20
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-04-20
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2018-03-05
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-03-05
Question
Q: is it okay to use EDTA plasma as samples in Mouse Resistin Picokine® ELISA Kit (Catalog # EK0582)?
Verified Customer
Verified customer
Asked: 2017-06-10
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of Resistin causing degradation of its protein structure. Resistin may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the Resistin ELISA, treating samples with various anticoagulants and decided that heparin can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2017-06-10
Question
Q: What is the optimal O.D. value for Resistin ELISA kit? I performed your Resistin ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain Resistin even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2014-02-13
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Resistin ELISA kit to have sensitivity of 2pg/ml, that means the minimum amount of Resistin that can be declared/interpreted as positive by the above standard is 2pg/ml.
Boster Scientific Support
Answered: 2014-02-13
