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Product Info Summary
| SKU: | EK0662 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse TNFRSF17/BCMA ELISA Kit PicoKine®
View all BCMA/TNFRSF17 ELISA kits
SKU/Catalog Number
EK0662
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse TNFRSF17/BCMA ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Tnfrsf17 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse TNFRSF17/BCMA ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0662)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: M1-T49
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0662 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0662 is reactive to TNFRSF17 in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0662 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of BCMA/TNFRSF17
TNFRSF17 (Tumor necrosis factor receptor superfamily member 17), also called BCMA, is a protein that in humans is encoded by the TNFRSF17 gene. The protein encoded by this gene is a member of the TNF-receptor superfamily and is mapped to 16p13.13. This receptor is preferentially expressed in mature B lymphocytes, and may be important for B cell development and autoimmune response. This receptor has been shown to specifically bind to the tumor necrosis factor (ligand) superfamily, member 13b (TNFSF13B/TALL-1/BAFF), and to lead to NF-kappaB and MAPK8/JNK activation. This receptor also binds to various TRAF family members, and thus may transduce signals for cell survival and proliferation.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0662-CAP | Anti-Mouse TNFRSF17 Pre-coated 96-well strip microplate | 1 |
| EK0662-ST | Mouse TNFRSF17 Standard | 2 vials, 10 ng/tube |
| EK0662-DA | Mouse TNFRSF17 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.004 | 0.032 | 0.097 | 0.186 | 0.374 | 0.738 | 1.117 | 1.834 |
Data Example Images
Click image to see more details
Mouse TNFRSF17/BCMA PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect BCMA/TNFRSF17, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 31.2 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse TNFRSF17/BCMA ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 82 | 288 | 757 | 87 | 276 | 787 |
| Standard deviation | 3.69 | 11.52 | 56.77 | 4.87 | 12.97 | 72.4 |
| CV (%) | 4.5% | 4% | 7.5% | 5.6% | 4.7% | 9.2% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of BCMA/TNFRSF17 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 82 | 85 | 83 | 96 | 86 | 5.59 | 6.5% |
| Sample 2 | 288 | 282 | 296 | 300 | 291 | 6.98 | 2.3% |
| Sample 3 | 757 | 861 | 852 | 799 | 817 | 42.08 | 5.1% |
Protein Target Info & Infographic
Gene/Protein Information For TNFRSF17 (Source: Uniprot.Org, NCBI)
Gene Name
TNFRSF17
Full Name
Tumor necrosis factor receptor superfamily member 17
Weight
20.165kDa
Alternative Names
B cell maturation antigen; B-cell maturation protein; BCMA; BCMAtumor necrosis factor receptor superfamily member 17; BCMB-cell maturation factor; CD269 antigen; CD269; TNFRSF13A; TNFRSF17; tumor necrosis factor receptor superfamily, member 17 TNFRSF17 BCM, BCMA, CD269, TNFRSF13A TNF receptor superfamily member 17 tumor necrosis factor receptor superfamily member 17|B cell maturation antigen|B-cell maturation factor|B-cell maturation protein
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on TNFRSF17, check out the TNFRSF17 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for TNFRSF17: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse TNFRSF17/BCMA ELISA Kit PicoKine® (EK0662)
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No publications found for EK0662
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Mouse TNFRSF17/BCMA ELISA Kit PicoKine®
Question
What dilution did you test mouse serum and plasma at using EK0662? Did you get native signal, if so how much of the protein was there? Or did you have to spike the samples to get signal?
Verified customer
Asked: 2021-02-18
Answer
For the Mouse TNFRSF17/BCMA ELISA Kit PicoKine™ (EK0662), the expression level of TNFRSF17 in normal healthy mouse samples is very low. Our lab used dilution of 1:1 and detected concentration was lower than 31.2pg/ml. We didn't spike the samples.
Boster Scientific Support
Answered: 2021-02-22
Question
Q: if the enzyme conjugated BCMA antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the BCMA antigen?
Verified Customer
Verified customer
Asked: 2020-06-05
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-06-05
Question
Q: What is the optimal O.D. value for BCMA ELISA kit? I performed your BCMA ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain BCMA even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2019-05-23
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this BCMA ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of BCMA that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2019-05-23
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2018-11-24
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-11-24
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2016-08-26
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2016-08-26
Question
Q: Can BCMA ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2014-03-15
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2014-03-15
