- Table of Contents
With great versatility (in FACS panels and protocols), comes great responsibility in choosing the right controls. Take a quick look to make sure you have included all the appropriate ones! Here’s a convenient mini guide to FACS staining controls:
|Control||1o Antibody||2o Antibody||Why use it?|
|Unstained||+||+||To subtract autofluorescence|
|Unstimulated||+/−||+/−||To set baseline cytokine/phosphoprotein expression|
|1 o/2 o Ab treated negative cells||+/−||+/−||To check for cross-reactivity of the antibodies|
|Isotype||Only isotype||+ for indirect staining||To subtract non-specific Fc receptor binding of 1 oAb|
|Compensation for every fluorochrome||+||+ for indirect staining||To subtract spectral overlap/multichannel spillage|
|FMO for every fluorochrome||+||+ for indirect staining||To subtract the signal of all non-specific fluorochromes from a specific channel|
|Viability||-||-||Live/dead cell discrimination|
Download troubleshootingnhandbooks for IHC, Western blot and ELISA for FREE.Troubleshooting guides
All Boster antibodies and ELISA kits are guaranteed to meet the specifications on the data sheet. We promise to thoroughly investigate any concerns about the quality of our products; if you encounter a problem with a Boster antibody or ELISA kit, our technical support team will respond with personalized advice within 24 hours. If we can’t make your experiment work, we will refund your purchase in full or provide a replacement free of charge.
Review : "This is an excellent antibody to endoplasmin in HC11 cells. Clean, reliable detection with very little if any background. Great antibody for Westerns. Could probably be optimized for 1 hour primary incubation."
Here are some quick facts about bosterbio.com