Introduction
The antibody recognizes CD7, a human T and NK lymphocyte antigen of 40 kDa. The CD7 antigen is expressed
throughout T lymphocyte differentiation, and is present on 85% to 90% of peripheral blood T lymphocytes. In
normal individuals, the CD7 antibody reacts with all CD8+
lymphocytes, approximately 90% of CD4+
lymphocytes,
and most NK lymphocytes. CD7 is weakly reactive with monocytes and does not react with granulocytes or B
lymphocytes. It is expressed on 50% of thymocytes in suspension. In leukemias, the CD7 antigen is present on the
majority of T lymphoid lineages.
This antibody is routinely tested by flow cytometric analysis. Flow cytometry and other applications were tested during antibody development by CapricoBio or are reported in the literature.
Application Information
Each lot of this antibody has been quality control tested by flow cytometric analysis of human PBMCs. For flow
cytometric staining, the recommended dose of this antibody is 0.5μg per 1x106
cells in 100μl staining volume
followed by staining with any fluorescent conjugated streptavidin. However, it is strongly suggested that the
antibody reactivity be empirically titrated for optimal performance in the application of interest.
Overview
| Product Name |
Mouse anti-human CD7 Monoclonal Antibody Biotin Conjugated, Flow Validated
See more CD7 products |
| Catalog# |
FC01974-1-Biotin |
| Pack Size |
100µg |
| Storage & Handling |
Store at 4°C for one year. Do not freeze. |
| Description |
Boster Bio Mouse anti-human CD7 Monoclonal Antibody Biotin Conjugated, Flow Validated catalog # FC01974-1-Biotin. Tested in Flow Cytometry applications. This antibody reacts with Human. Supplied as 100µg in Liquid form antibody. |
| Cite This Product |
Mouse anti-human CD7 Monoclonal Antibody Biotin Conjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC01974-1-Biotin)
|
| Antibodies Validation |
Antibodies Validation Information
|
Similar Products From
Other Companies |
Mouse anti-human CD7 Monoclonal Antibody Biotin Conjugated, Flow Validated may replace the following items: sc 6993|sc 53568|sc 6992|sc 19645|sc 7631|sc 28332|sc 14004|sc 48409|sc 59108|sc 6991. |
Product Specs
| Host |
Mouse |
| Reactive Species |
Human |
| Applications |
Flow Cytometry
*Our Boster Guarantee covers the use of this product in the above
tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products. |
| Immunogen |
Human Leukocytes. |
Gene/Protein Basic Information For CD7 (Source: Uniprot.org, NCBI)
| Uniprot Id | P09564 |
|---|
| NCBI Gene Id | 924 |
|---|
| Species Of This Entry | Human |
|---|
| Gene Name | CD7 |
|---|
| Protein Name | T-cell antigen CD7 |
|---|
| Alternative Names | CD7|CD7 antigen (p41); CD7 antigen; CD7 molecule; CD7; GP40T-cell surface antigen Leu-9; LEU-9; T-cell antigen CD7; T-cell leukemia antigen; Tp40; TP41; TP41p41 protein |
|---|
| Molecular Weight | 25409 |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on CD7, check out the CD7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for CD7: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the CD7 infographic
Our Boster Quality Guarantee for Mouse anti-human CD7 Monoclonal Antibody Biotin Conjugated, Flow Validated covers its use in the following applications.
User need to optimize the dilution ratio for this antibody
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Lymphocytes gated PBMCs stained with
biotin conjugated anti-human CD7 (clone
4H9) followed by staining with SA-PE (red
histogram). Lymphocytes gated PBMCs
stained with biotin conjugated mouse IgG2a
isotype control followed by staining with SAPE
(Blue histogram).
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Did You Know ?
That Boster Bio offers comprehensive selection of Western blot and IHC reagents? Great quality and save up to 50% cost.
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Total number of citations: 1
| Human papillomavirus genotypes and p16 expression in oral leukoplakia and squamous cell carcinoma
Li-Qun Yang,1,2,3,4,* Xuan Xiao,5,* Chen-Xi Li,1,2 Wen-Yan Wu,1,2 Xue-Min Shen,1,2 Zeng-Tong Zhou,1,2 Yuan Fan,3,4 Lin-Jun Shi1,2
Int J Clin Exp Pathol. |
PubMed ID 31933914 |
|
0 reviews
Contact us with any questions at [email protected] or go to contact us
Questions and answers from customers related to FC01974-1-Biotin Mouse anti-human CD7 Monoclonal Antibody Biotin Conjugated, Flow Validated
11 Related Questions
Question
Will FC01974-1-Biotin Mouse anti-human CD7 Monoclonal antibody Biotin Conjugated, Flow Validated work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Asked: 2019-08-26
Answer
You can see on the product datasheet, FC01974-1-Biotin Mouse anti-human CD7 Monoclonal antibody Biotin Conjugated, Flow Validated as been tested on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-26
Question
See attached the WB image, lot number and protocol we used for muscle using Mouse anti-human CD7 Monoclonal antibody Biotin Conjugated, Flow Validated FC01974-1-Biotin. Please let me know if you require anything else.
Verified Customer
Asked: 2019-08-15
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-15
Question
Do you have a BSA free version of Mouse anti-human CD7 Monoclonal antibody Biotin Conjugated, Flow Validated FC01974-1-Biotin available?
Verified Customer
Asked: 2019-05-31
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this Mouse anti-human CD7 Monoclonal antibody Biotin Conjugated, Flow Validated FC01974-1-Biotin are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-31
Question
I see that the Mouse anti-human CD7 Monoclonal antibody Biotin Conjugated, Flow Validated FC01974-1-Biotin works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
P. Anderson
Asked: 2018-10-11
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-10-11
Question
Is this FC01974-1-Biotin Mouse anti-human CD7 Monoclonal antibody Biotin Conjugated, Flow Validated reactive to the isotypes of CD7?
Verified Customer
Asked: 2018-08-02
Answer
The immunogen of FC01974-1-Biotin Mouse anti-human CD7 Monoclonal antibody Biotin Conjugated, Flow Validated is Human Leukocytes. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-08-02
Question
Will Mouse anti-human CD7 Monoclonal antibody Biotin Conjugated, Flow Validated FC01974-1-Biotin work for Flow Cytometry with muscle?
Verified Customer
Asked: 2018-05-24
Answer
According to the expression profile of muscle, CD7 is highly expressed in muscle. So, it is likely that Mouse anti-human CD7 Monoclonal antibody Biotin Conjugated, Flow Validated FC01974-1-Biotin will work for Flow Cytometry with muscle.
Boster Scientific Support
Answered: 2018-05-24
Question
I would like to test Mouse anti-human CD7 Monoclonal antibody Biotin Conjugated, Flow Validated FC01974-1-Biotin on human muscle for research purposes, then I may be interested in using Mouse anti-human CD7 Monoclonal antibody Biotin Conjugated, Flow Validated FC01974-1-Biotin for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Asked: 2017-12-26
Answer
The products we sell, including Mouse anti-human CD7 Monoclonal antibody Biotin Conjugated, Flow Validated FC01974-1-Biotin, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-12-26
Question
I was wanting to use your Mouse anti-human CD7 Monoclonal antibody Biotin Conjugated, Flow Validated for Flow Cytometry for human muscle on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human muscle identification?
Verified Customer
Asked: 2017-12-11
Answer
It shows on the product datasheet, FC01974-1-Biotin Mouse anti-human CD7 Monoclonal antibody Biotin Conjugated, Flow Validated has been tested for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human muscle in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-12-11
Question
I have a question about product FC01974-1-Biotin, Mouse anti-human CD7 Monoclonal antibody Biotin Conjugated, Flow Validated. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Jones
Asked: 2014-04-24
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free FC01974-1-Biotin Mouse anti-human CD7 Monoclonal antibody Biotin Conjugated, Flow Validated, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-04-24
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for muscle using Mouse anti-human CD7 Monoclonal antibody Biotin Conjugated, Flow Validated FC01974-1-Biotin. Let me know if you need anything else.
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-03-13
Question
Is a blocking peptide available for product Mouse anti-human CD7 Monoclonal antibody Biotin Conjugated, Flow Validated (FC01974-1-Biotin)?
T. Krishna
Asked: 2013-07-31
Answer
We do provide the blocking peptide for product Mouse anti-human CD7 Monoclonal antibody Biotin Conjugated, Flow Validated (FC01974-1-Biotin). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-07-31
