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Greetings Earthling,
Did you know...The mantis shrimp has SIXTEEN color-receptive cones which allow it to see a MASSIVE spectrum of color that human brains aren't even capable of processing (we only have 3 types of cones). Good thing the mantis shrimp will never get to design multicolor flow cytometry antibody panels... and good thing Boster has 6 helpful tips for humans to make multicolor panels right here! Click the shrimps for more FACS resources.
Troubleshooting Guides
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Troubleshooting guidesFACS antibodies multicolor panel design
- Always try to pair the brightest fluorochromes with the weakest expressing antigen. In case the expression level of an antigen is unknown, it is advisable to use brighter fluorochromes.
- Avoid spillover or spectral overlap between fluorochromes by spreading them as much as possible across the spectrum.
- Avoid fluorochromes that can be excited by more than one laser e.g. APC-Cy7.
- Always include suitable compensation controls (see our FACS resources for details) especially if points 3 and 4 above cannot be achieved.
- It is also advisable to use FMO controls (see our FACS resources for details) to gate differently stained sub-populations more accurately.
- Consider using online multicolor panel designers provided by FACS technology companies e.g. fluorofinder.com.
Example: A suitable 4-color panel to illustrate points 1-3. The color of the boxes correspond to the lasers that excite the respective fluorochromes.
These CD antibodies are available from Boster! Check recommended products below for validated FACS images...
Boster's Available Fluorophores (antibody conjugate labels):
*Note to educators: you are encouraged to share BosterBio's resources and PDFs on your class and lab websites, please cite or link to origin bosterbio.com
FACS validated antibodies, click here: Shop now!
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Boster Products for Flow Cytometry
Anti-Human CD45 PTPRC Monoclonal Antibody PerCP Conjugated, Flow Validated
| SKU | FC00555-PerCP |
|---|---|
| Reactivity | Human |
| Applications | Flow Cytometry |
Anti-Human CD3 Cd3e Monoclonal Antibody FITC Conjugated, Flow Validated
| SKU | FC02675-FITC |
|---|---|
| Reactivity | Human |
| Applications | Flow Cytometry |
Anti-Human CD19 Monoclonal Antibody APC Conjugated, Flow Validated
| SKU | FC00154-APC |
|---|---|
| Reactivity | Human |
| Applications | Flow Cytometry |
Anti-Human CD45 PTPRC Monoclonal Antibody APC Conjugated, Flow Validated
| SKU | FC00555-APC |
|---|---|
| Reactivity | Human |
| Applications | Flow Cytometry |
Anti-Human CD4 Monoclonal Antibody APC Conjugated, Flow Validated
| SKU | FC00344-APC |
|---|---|
| Reactivity | Human |
| Applications | Flow Cytometry |
Anti-Human CD19 Monoclonal Antibody PE Conjugated, Flow Validated
| SKU | FC00154-PE |
|---|---|
| Reactivity | Human |
| Applications | Flow Cytometry |
Boster Guarantee
All Boster antibodies and ELISA kits are guaranteed to meet the specifications on the data sheet. We promise to thoroughly investigate any concerns about the quality of our products; if you encounter a problem with a Boster antibody or ELISA kit, our technical support team will respond with personalized advice within 24 hours. If we can’t make your experiment work, we will refund your purchase in full or provide a replacement free of charge.
Product Review
Anti-TNF Alpha Antibody (PB9246)
Review : "Under these experimental conditions...[TNF-α P28 Boster anti-CD14] demonstrated positive staining of the THP-1 cells."
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