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Product Info Summary
|Description:||Transient overexpression lysate of RAN, member RAS oncogene family (RAN)|
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RAN (NM_006325) Human Over-expression Lysate
Transient overexpression lysate of RAN, member RAS oncogene family (RAN)
Storage & Handling
Cite This Product
RAN (NM_006325) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS005901)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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RAN Human Over-expression Lysates NM_006325.
Western blot validation of overexpression lysate (LS005901) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RAN (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for RAN (Source: Uniprot.org, NCBI)
GTP-binding nuclear protein Ran
small GTPase superfamily
Androgen receptor-associated protein 24; ARA24Gsp1; GTPase Ran; GTP-binding nuclear protein Ran; guanosine triphosphatase Ran; member RAS oncogene family; OK/SW-cl.81; RAN, member RAS oncogene family; RanGTPase; Ras-like protein TC4; Ras-related nuclear protein; TC4 RAN ARA24, Gsp1, TC4 RAN, member RAS oncogene family GTP-binding nuclear protein Ran|GTPase Ran|RanGTPase|androgen receptor-associated protein 24|guanosine triphosphatase Ran|member RAS oncogene family|ras-like protein TC4|ras-related nuclear protein*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
For more info on RAN, check out the RAN Infographic
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No publications found for LS005901
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