Rat MIP-1Alpha/CCL3 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Rat MIP-1Alpha/CCL3 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Rat Ccl3 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 1 pg/ml.

Product Info Summary

SKU: EZ1219
Size: For 5 plates, 96 wells each
Reactive Species: Rat
Application: ELISA

Product Name

Rat MIP-1Alpha/CCL3 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

See all Ccl3 products

SKU/Catalog Number

EZ1219

Size

For 5 plates, 96 wells each

*Question: How many samples can I assay/run in this kit?

Description

Rat MIP-1Alpha/CCL3 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Rat Ccl3 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 1 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Rat MIP-1Alpha/CCL3 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ1219)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A24-A92

Sensitivity

<1 pg/ml

Assay Range

7.8 pg/ml - 500 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EZ1219 is reactive to Ccl3 in Rat samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Application Guarantee

EZ1219 is guaranteed for ELISA in Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog number Description Quantity Buffers
EZ1219-CA Rabbit anti- rat Ccl3 polyclonal antibody (Capture Antibody) 500 µl, 0.4 mg/mL (recommended dilution 1:100) 0.5% Na2HPO4, 0.04% Proclin 300 and 50% Glycerol
EZ1219-DA Biotinylated goat anti- rat Ccl3 polyclonal antibody (Detection Antibody) 500 µl, 3.3 µg/mL (recommended dilution 1:100) 0.04% Proclin 300, 0.5% BSA, 0.2% Tris, 1% NaCl and 30% Glycerol
EZ1219-ST Lyophilized recombinant rat Ccl3 standard 10 ng/tube×2
AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 µl (recommended dilution 1:100)

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • 1. Microplate reader in standard size.
  • 2. Automated plate washer.
  • 3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • 4. Clean tubes and Eppendorf tubes.
  • 5. 96 well microplate (Cat# AR1100)
  • 6. Plate Sealers.
  • 7. Capture Antibody Diluent: PBS.
  • 8. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
  • 9. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
  • 10. Stop Solution: 2 N H2SO4 (Cat# AR1105)
  • 11. Wash Buffer (PBS and PBS-T). Recipes:
    PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
    PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
  • *Item 5 – 11 are included in the EZ Set Accessory Kit (EZA001)

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)07.815.631.362.5125250500
O.D.0.1370.2180.2830.4220.6911.1011.8162.423

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)5317729365192317
Standard deviation2.7611.8613.24.114.618.4
CV (%)5.2%6.7%4.5%6.3%7.6%5.8%

Gene/Protein Information For Ccl3 (Source: Uniprot.Org, NCBI)

Uniprot ID

P50229

Gene ID

25542

Gene Name

Ccl3

Full Name

C-C motif chemokine 3

Weight

10.335kDa

Superfamily

intercrine beta (chemokine CC) family

Alternative Names

C-C motif chemokine 3; MIP1-(a); AI323804; CCL3; chemokine (C-C motif) ligand 3; G0S19-1; LD78a; LD78alpha; MIP1 alpha; MIP-1 alpha; MIP1A; MIP-1alpha; MIP1-alpha; PAT 464.1; SCYA3; SIS-beta Ccl3|MIP-1a, Scya3|C-C motif chemokine ligand 3|C-C motif chemokine 3|MIP-1-alpha|Macrophage inflammatory protein 1 alpha (Small inducible cytokine A3)|chemokine (C-C motif) ligand 3|macrophage inflammatory protein 1-alpha|small-inducible cytokine A3

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Ccl3, check out the Ccl3 Infographic

Ccl3 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Ccl3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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1 Customer Q&As for Rat MIP-1Alpha/CCL3 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Question

What is the concentration of the capture antibody for EZ1219?

Verified customer

Asked: 2019-04-23

Answer

The concentration of the capture antibody of the Rat MIP-1Alpha/CCL3 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (EZ1219) is 0.4mg/ml.

Boster Scientific Support

Answered: 2019-04-24

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