RSK1/2/3/4 Colorimetric Cell-Based ELISA Kit

RSK1 ELISA kit, cell-based

The RSK1/2/3/4 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor RSK1/2/3/4 protein expression profile in cells. The kit can be used for measuring the relative amounts of RSK1/2/3/4 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on RSK1/2/3/4.

Product Info Summary

SKU: EKC1508
Size: 1 kit, containing one 96-well plate and all necessary reagents
Reactive Species: Human, Mouse
Application: ELISA

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Product Name

RSK1/2/3/4 Colorimetric Cell-Based ELISA Kit

See all RSK1 products

SKU/Catalog Number

EKC1508

Size

1 kit, containing one 96-well plate and all necessary reagents

Description

The RSK1/2/3/4 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor RSK1/2/3/4 protein expression profile in cells. The kit can be used for measuring the relative amounts of RSK1/2/3/4 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on RSK1/2/3/4.

Storage & Handling

Store at 4°C for up to 6 months.

Cite This Product

RSK1/2/3/4 Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1508)

Clonality of Antibodies

See Datasheet for details

Assay Range

> 5000 cells/well

Reactive Species

EKC1508 is reactive to RPS6KA1 in Human, Mouse samples

Application Guarantee

EKC1508 is guaranteed for ELISA in Human, Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 1 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-RSK1 Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 6ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 6ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 6ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

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Gene/Protein Information For RPS6KA1 (Source: Uniprot.Org, NCBI)

Gene Name

RPS6KA1

Full Name

Ribosomal protein S6 kinase alpha-1

Weight

82.723kDa

Superfamily

protein kinase superfamily

Alternative Names

90kD, polypeptide 1); EC 2.7.11; EC 2.7.11.1,90 kDa ribosomal protein S6 kinase 1; HU-1; MAP kinase-activated protein kinase 1a; MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a; MAPKAPL1A; p90RSK1; p90S6K; ribosomal protein S6 kinase alpha 1; ribosomal protein S6 kinase alpha-1; ribosomal protein S6 kinase, 90kD, polypeptide 1; ribosomal protein S6 kinase, 90kDa, polypeptide 1; Ribosomal S6 kinase 1; RPS6KA1; RSK; RSK1; RSK-1; RSK1p90-RSK 1; S6K-alpha 1; S6K-alpha-1 RPS6KA1 HU-1, MAPKAPK1, MAPKAPK1A, RSK, RSK1, p90Rsk ribosomal protein S6 kinase A1 ribosomal protein S6 kinase alpha-1|90 kDa ribosomal protein S6 kinase 1|MAP kinase-activated protein kinase 1a|MAPK-activated protein kinase 1a|MAPKAP kinase 1a|MAPKAPK-1a|RSK-1|S6K-alpha 1|dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1)|p90-RSK 1|p90RSK1|p90S6K|ribosomal S6 kinase 1|ribosomal protein S6 kinase, 90kDa, polypeptide 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on RPS6KA1, check out the RPS6KA1 Infographic

RPS6KA1 infographic

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In this infographic you will see the following information for RPS6KA1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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