Anti-14-3-3 epsilon YWHAE Antibody

Product Name

Anti-14-3-3 epsilon YWHAE Antibody

View all 14-3-3 epsilon Antibodies

SKU/Catalog Number

A01687-3

Size

100ul

Form

Liquid

Description

Boster Bio Anti-14-3-3 epsilon YWHAE Antibody catalog # A01687-3. Tested in WB,ICC/IF,IHC,Flow Cytometry applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-14-3-3 epsilon YWHAE Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01687-3)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human DNA Ligase IV around the phosphorylation site of T650.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A01687-3 is reactive to YWHAE in Human, Mouse, Rat

Applications

A01687-3 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

29.174kDa

Background of 14-3-3 epsilon

Regulates and initiates biosynthesis of N-acetylneuraminic acid (NeuAc), a precursor of sialic acids. Plays an essential role in early development By similarity. Required for normal sialylation in hematopoietic cells. Sialylation is implicated in cell adhesion, signal transduction, tumorigenicity and metastatic behavior of malignant cells.

Lucka L., FEBS Lett. 454:341-344(1999).
Seppala R., Am. J. Hum. Genet. 64:1563-1569(1999).
Huizing M., Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1,000-1:2,000

ICC: 1:50-1:200
IHC: 1:50-1:200
FC: 1:10-1:100

Validation Images & Assay Conditions

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Figure 2. Immunocytochemistry staining of YWHAE using Anti-14-3-3 epsilon YWHAE Antibody (A01687-3).

ICC staining 14-3-3 epsilon in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde

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Figure 1. Western blotting validation for Anti-14-3-3 epsilon YWHAE Antibody A01687-3

Western blot analysis of 14-3-3 epsilon on different lysates using anti-14-3-3 epsilon antibody at 1/1

Gene/Protein Information For YWHAE (Source: Uniprot.org, NCBI)

Alternative Names

1433 epsilon; 14-3-3 epsilon; 14-3-3 protein epsilon; FLJ45465; FLJ53559,14-3-3E; KCIP-1; MDCR; MDS; mitochondrial import stimulation factor L subunit; protein kinase C inhibitor protein-1; tyrosine 3/tryptophan 5 -monooxygenase activation protein, epsilon polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilonpolypeptide; YWHAE YWHAE 14-3-3E, HEL2, KCIP-1, MDCR, MDS tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon 14-3-3 protein epsilon|14-3-3 epsilon|epididymis luminal protein 2|mitochondrial import stimulation factor L subunit|protein kinase C inhibitor protein-1|tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide|tyrosine 3/tryptophan 5 -monooxygenase activation protein, epsilon polypeptide

For more info on YWHAE, check out the YWHAE Infographic

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In this infographic, you will see the following information for YWHAE: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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