Product Info Summary
SKU: | M02383 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-ACADM/Mcad Rabbit Monoclonal Antibody
SKU/Catalog Number
M02383
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-ACADM/Mcad Rabbit Monoclonal Antibody catalog # M02383. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ACADM/Mcad Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02383)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
COE-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human ACADM
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02383 is reactive to ACADM in Human, Mouse, Rat
Applications
M02383 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
85 kDa
Calculated molecular weight
46.588kDa
Background of ACADM
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of ACADM expression in (1) HeLa cell lysate; (2) K562 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For ACADM (Source: Uniprot.org, NCBI)
Gene Name
ACADM
Full Name
Medium-chain specific acyl-CoA dehydrogenase, mitochondrial
Weight
46.588kDa
Superfamily
acyl-CoA dehydrogenase family
Alternative Names
ACAD1; acyl-CoA dehydrogenase, C-4 to C-12 straight chain; acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain; EC 1.3.99; EC 1.3.99.3; FLJ18227; FLJ99884; MCADFLJ93013; MCADH; medium-chain specific acyl-CoA dehydrogenase, mitochondrial ACADM ACAD1, MCAD, MCADH acyl-CoA dehydrogenase medium chain medium-chain specific acyl-CoA dehydrogenase, mitochondrial|acyl-CoA dehydrogenase, C-4 to C-12 straight chain|acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain|medium-chain acyl-CoA dehydrogenase|testicular tissue protein Li 7
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ACADM, check out the ACADM Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ACADM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ACADM/Mcad Rabbit Monoclonal Antibody (M02383)
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No publications found for M02383
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Recommended Resources
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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7 Customer Q&As for Anti-ACADM/Mcad Rabbit Monoclonal Antibody
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for heart using anti-ACADM/Mcad Rabbit Monoclonal antibody M02383. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-10
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-10
Question
Is there a BSA free version of anti-ACADM/Mcad Rabbit Monoclonal antibody M02383 available?
Verified Customer
Verified customer
Asked: 2019-06-24
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ACADM/Mcad Rabbit Monoclonal antibody M02383 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-24
Question
I was wanting to use your anti-ACADM/Mcad Rabbit Monoclonal antibody for WB for human heart on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human heart identification?
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
As indicated on the product datasheet, M02383 anti-ACADM/Mcad Rabbit Monoclonal antibody has been validated for IP, IF, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human heart in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-16
Question
My question regarding product M02383, anti-ACADM/Mcad Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-10-11
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02383 anti-ACADM/Mcad Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-10-11
Question
Here is the WB image, lot number and protocol we used for heart using anti-ACADM/Mcad Rabbit Monoclonal antibody M02383. Please let me know if you require anything else.
A. Huang
Verified customer
Asked: 2017-07-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-21
Question
We are currently using anti-ACADM/Mcad Rabbit Monoclonal antibody M02383 for mouse tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
K. Collins
Verified customer
Asked: 2015-05-21
Answer
The anti-ACADM/Mcad Rabbit Monoclonal antibody (M02383) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-05-21
Question
Does anti-ACADM/Mcad Rabbit Monoclonal antibody M02383 work on canine IHC with colon?
L. Walker
Verified customer
Asked: 2013-11-05
Answer
Our lab technicians have not validated anti-ACADM/Mcad Rabbit Monoclonal antibody M02383 on canine. You can run a BLAST between canine and the immunogen sequence of anti-ACADM/Mcad Rabbit Monoclonal antibody M02383 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine colon in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-11-05