Product Info Summary
SKU: | MA1000 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Chicken, Human, Mouse, Rat |
Host: | Mouse |
Application: | IHC, WB |
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Product info
Product Name
Anti-Actin ACTA1 Antibody (Monoclonal, AC-40)
SKU/Catalog Number
MA1000
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Actin ACTA1 Antibody (Monoclonal, AC-40) catalog # MA1000. Tested in IHC, WB applications. This antibody reacts with Chicken, Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Actin ACTA1 Antibody (Monoclonal, AC-40) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1000)
Host
Mouse
Contents
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Clonality
Monoclonal
Clone Number
AC-40
Isotype
Mouse IgG2a
Immunogen
Synthetic actin C-terminal peptideSer-Gly-Pro-Ser-Ile-Val-His-Arg-Lys-Cys-Phe,attached to a Multiple Antigen Peptide (MAP) backbone.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1000 is reactive to ACTA1 in Chicken, Human, Mouse, Rat
Applications
MA1000 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
43 kDa
Calculated molecular weight
42.051kDa
Background of ACTA1
Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in the cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha-actin, one beta-actin, two gamma-actin. Because the amino acid sequence of the C-terminal is the same for almost all actins, this antibody has been raised using a synthetic peptide corresponding to the C-terminal 11 residues.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Frozen Section), 4μg/ml, Human, chicken, mouse, rat, -
Western blot, 2μg/ml, Human, chicken, mouse, rat
Validation Images & Assay Conditions
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Anti-Actin antibody, MA1000, IHC(F)
IHC(F): Mouse Intestine Tissue
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Anti-Actin antibody, MA1000, IHC(F)
IHC(F): Human Placenta Tissue
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Anti-Actin antibody, MA1000, IHC(F)
IHC(F): Rat Spleen Tissue
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Anti-Actin antibody, MA1000, IHC(F)
IHC(F): Mouse Spleen Tissue
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Anti-Actin antibody, MA1000, IHC(F)
IHC(F): Rat Intestine Tissue
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Figure 6. Western blot analysis of Actin using anti-Actin antibody (MA1000).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HEK293 whole cell lysate,
Lane 2: rat PC-12 whole cell lysate,
Lane 3: mouse NIH3T3 cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Actin antigen affinity purified monoclonal antibody (Catalog # MA1000) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Actin at approximately 43KD. The expected band size for Actin is at 43KD.
Protein Target Info & Infographic
Gene/Protein Information For ACTA1 (Source: Uniprot.org, NCBI)
Gene Name
ACTA1
Full Name
Actin, alpha skeletal muscle
Weight
42.051kDa
Superfamily
actin family
Alternative Names
ACTA; Actin; actin, alpha 1, skeletal muscle; alpha skeletal muscle actin; alpha skeletal muscle; alpha-actin-1; ASMA; CFTD; CFTDM; MPFD; NEM1; NEM2; NEM3 ACTA1 ACTA, ASMA, CFTD, CFTD1, CFTDM, MPFD, NEM1, NEM2, NEM3, SHPM actin alpha 1, skeletal muscle actin, alpha skeletal muscle|nemaline myopathy type 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ACTA1, check out the ACTA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ACTA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Actin ACTA1 Antibody (Monoclonal, AC-40) (MA1000)
Hello CJ!
MA1000 has been cited in 10 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The mTOR-glycolytic pathway promotes T-cell immunobiology in oral lichen planus
Liao L,Huang L,Wei X,Yin L,Wei X,Li T.Bioinformatic and biochemical studies of formononetin against liver injure.Life Sci.2021 Feb 16:119229.doi:10.1016/j.lfs.2021.119229.Epub ahead of print.PMID:33607154.
Species: Mouse
MA1000 usage in article: APP:WB, SAMPLE:LIVER TISSUE, DILUTION:NA
Liao L,Huang L,Wei X,Yin L,Wei X,Li T.Bioinformatic and biochemical studies of formononetin against liver injure.Life Sci.2021 Feb 16:119229.doi:10.1016/j.lfs.2021.119229.Epub ahead of print.PMID:33607154.
Species: Mouse
MA1000 usage in article: APP:WB, SAMPLE:LIVER TISSUE, DILUTION:NA
Cheng J,Luan J,Chen P,Kuang X,Jiang P,Zhang R,Chen S,Cheng F,Gou X.Immunosuppressive receptor LILRB1 acts as a potential regulator in hepatocellular carcinoma by integrating with SHP1.Cancer Biomark.2020; 28(3):309-319.doi:10.3233/CBM-190940.PMID:32390601
Species: Human
MA1000 usage in article: APP:WB, SAMPLE:LO2,MHCC97L,HUH 7 AND HEPG2 CELLS, DILUTION:NA
Sandulescu T, Weniger J, Philippou S, Mücke T, Naumova EA, Arnold WH. Immunohistochemical evidence of striated muscle cells within midfacial superficial musculoaponeurotic system. Ann Anat. 2020 Nov 19:151647. doi: 10.1016/j.aanat.2020.151647. Epub ahead
Species: Human
MA1000 usage in article: APP:IHC, SAMPLE:SMAS FIBROUS SEPTA, DILUTION:NA
Hydrogen molecules (H2) improve perfusion recovery via antioxidant effects in experimental peripheral arterial disease
Transplantation of parthenogenetic embryonic stem cells ameliorates cardiac dysfunction and remodelling after myocardial infarction
Contribution of the PI3K/MMPs/Ln-5%u03B32 and EphA2/FAK/Paxillin signaling pathways to tumor growth and vasculogenic mimicry of gallbladder carcinomas
Sesamin ameliorates high-fat diet%u2013induced dyslipidemia and kidney injury by reducing oxidative stress
Expression and significance of netrin%u20131 and its receptor UNC5C in precocious puberty female rat hypothalamus
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Customer Reviews
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Customer Q&As
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2 Customer Q&As for Anti-Actin ACTA1 Antibody (Monoclonal, AC-40)
Question
I am looking for using your anti-Actin antibody (Monoclonal, AC-40) for muscle contraction studies. Has this antibody been tested with western blotting on rat spleen tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-07-30
Answer
We appreciate your inquiry. This MA1000 anti-Actin antibody (Monoclonal, AC-40) is validated on mouse spleen tissue, intestine tissue, rat spleen tissue, human placenta tissue, nih3t3 cell lysate. It is guaranteed to work for IHC, WB in chicken, human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-07-30
Question
We ordered your Anti-Actin Antibody (Monoclonal, AC-40) for WB on skeletal muscle last year. I am using rat, and I plan to use the antibody for IHC next. We are interested in examining skeletal muscle as well as biceps brachii in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
M. Banerjee
Verified customer
Asked: 2015-01-06
Answer
I viewed the website and datasheets of our Anti-Actin Antibody (Monoclonal, AC-40) and it seems that MA1000 has been tested on rat. Thus MA1000 should work for your application. Our Boster satisfaction gurantee will cover this product for IHC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2015-01-06