Anti-Actin ACTA1 Antibody (Monoclonal, AC-40)

ACTA1 antibody

Boster Bio Anti-Actin ACTA1 Antibody (Monoclonal, AC-40) catalog # MA1000. Tested in IHC, WB applications. This antibody reacts with Chicken, Human, Mouse, Rat. Cited in 10 publication(s).

Product Info Summary

SKU: MA1000
Size: 100 μg/vial
Reactive Species: Chicken, Human, Mouse, Rat
Host: Mouse
Application: IHC, WB

Product Name

Anti-Actin ACTA1 Antibody (Monoclonal, AC-40)

View all ACTA1 Antibodies

SKU/Catalog Number

MA1000

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Actin ACTA1 Antibody (Monoclonal, AC-40) catalog # MA1000. Tested in IHC, WB applications. This antibody reacts with Chicken, Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Actin ACTA1 Antibody (Monoclonal, AC-40) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1000)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

AC-40

Isotype

Mouse IgG2a

Immunogen

Synthetic actin C-terminal peptideSer-Gly-Pro-Ser-Ile-Val-His-Arg-Lys-Cys-Phe,attached to a Multiple Antigen Peptide (MAP) backbone.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

MA1000 is reactive to ACTA1 in Chicken, Human, Mouse, Rat

Applications

MA1000 is guaranteed for IHC, WB Boster Guarantee

Background of ACTA1

Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in the cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha-actin, one beta-actin, two gamma-actin. Because the amino acid sequence of the C-terminal is the same for almost all actins, this antibody has been raised using a synthetic peptide corresponding to the C-terminal 11 residues.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Frozen Section), 4μg/ml, Human, chicken, mouse, rat, -
Western blot, 2μg/ml, Human, chicken, mouse, rat

Validation Images & Assay Conditions

Gene/Protein Information For ACTA1 (Source: Uniprot.org, NCBI)

Uniprot ID

P68133

Gene ID

58

Gene Name

ACTA1

Full Name

Actin, alpha skeletal muscle

Weight

42.051kDa

Superfamily

actin family

Alternative Names

ACTA; Actin; actin, alpha 1, skeletal muscle; alpha skeletal muscle actin; alpha skeletal muscle; alpha-actin-1; ASMA; CFTD; CFTDM; MPFD; NEM1; NEM2; NEM3 ACTA1 ACTA, ASMA, CFTD, CFTD1, CFTDM, MPFD, NEM1, NEM2, NEM3, SHPM actin alpha 1, skeletal muscle actin, alpha skeletal muscle|nemaline myopathy type 3

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ACTA1, check out the ACTA1 Infographic

ACTA1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ACTA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

MA1000 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

The mTOR-glycolytic pathway promotes T-cell immunobiology in oral lichen planus

Liao L,Huang L,Wei X,Yin L,Wei X,Li T.Bioinformatic and biochemical studies of formononetin against liver injure.Life Sci.2021 Feb 16:119229.doi:10.1016/j.lfs.2021.119229.Epub ahead of print.PMID:33607154.
Species: Mouse
MA1000 usage in article: APP:WB, SAMPLE:LIVER TISSUE, DILUTION:NA

Liao L,Huang L,Wei X,Yin L,Wei X,Li T.Bioinformatic and biochemical studies of formononetin against liver injure.Life Sci.2021 Feb 16:119229.doi:10.1016/j.lfs.2021.119229.Epub ahead of print.PMID:33607154.
Species: Mouse
MA1000 usage in article: APP:WB, SAMPLE:LIVER TISSUE, DILUTION:NA

Cheng J,Luan J,Chen P,Kuang X,Jiang P,Zhang R,Chen S,Cheng F,Gou X.Immunosuppressive receptor LILRB1 acts as a potential regulator in hepatocellular carcinoma by integrating with SHP1.Cancer Biomark.2020; 28(3):309-319.doi:10.3233/CBM-190940.PMID:32390601
Species: Human
MA1000 usage in article: APP:WB, SAMPLE:LO2,MHCC97L,HUH 7 AND HEPG2 CELLS, DILUTION:NA

Sandulescu T, Weniger J, Philippou S, Mücke T, Naumova EA, Arnold WH. Immunohistochemical evidence of striated muscle cells within midfacial superficial musculoaponeurotic system. Ann Anat. 2020 Nov 19:151647. doi: 10.1016/j.aanat.2020.151647. Epub ahead
Species: Human
MA1000 usage in article: APP:IHC, SAMPLE:SMAS FIBROUS SEPTA, DILUTION:NA

Hydrogen molecules (H2) improve perfusion recovery via antioxidant effects in experimental peripheral arterial disease

Transplantation of parthenogenetic embryonic stem cells ameliorates cardiac dysfunction and remodelling after myocardial infarction

Contribution of the PI3K/MMPs/Ln-5%u03B32 and EphA2/FAK/Paxillin signaling pathways to tumor growth and vasculogenic mimicry of gallbladder carcinomas

Sesamin ameliorates high-fat diet%u2013induced dyslipidemia and kidney injury by reducing oxidative stress

Expression and significance of netrin%u20131 and its receptor UNC5C in precocious puberty female rat hypothalamus

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2 Customer Q&As for Anti-Actin ACTA1 Antibody (Monoclonal, AC-40)

Question

I am looking for using your anti-Actin antibody (Monoclonal, AC-40) for muscle contraction studies. Has this antibody been tested with western blotting on rat spleen tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-07-30

Answer

We appreciate your inquiry. This MA1000 anti-Actin antibody (Monoclonal, AC-40) is validated on mouse spleen tissue, intestine tissue, rat spleen tissue, human placenta tissue, nih3t3 cell lysate. It is guaranteed to work for IHC, WB in chicken, human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-07-30

Question

We ordered your Anti-Actin Antibody (Monoclonal, AC-40) for WB on skeletal muscle last year. I am using rat, and I plan to use the antibody for IHC next. We are interested in examining skeletal muscle as well as biceps brachii in our next experiment. Do you have any suggestion on which antibody would work the best for WB?

M. Banerjee

Verified customer

Asked: 2015-01-06

Answer

I viewed the website and datasheets of our Anti-Actin Antibody (Monoclonal, AC-40) and it seems that MA1000 has been tested on rat. Thus MA1000 should work for your application. Our Boster satisfaction gurantee will cover this product for IHC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2015-01-06

Size

Conjugation

Total: $315

SKU:MA1000

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MA1000
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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$50 fee for conjugation. Antibody size is reduced to 50ug.

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