SKU MA1000
Size 100μg/vial
Reactivity Chicken, Human, Mouse, Rat
Clonality Monoclonal
Clone Number AC-40
Host Mouse
Ig Isotype Mouse IgG2a
Applications IHC, WB

Overview

Product Name Anti-Actin Antibody (Monoclonal, AC-40)
SKU/Catalog Number MA1000
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for Actin detection. Tested with WB, IHC-F in Human;mouse;rat;chicken. No cross reactivity with other proteins.
Cite This Product Anti-Actin Antibody (Monoclonal, AC-40) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1000)
Host Mouse
Immunogen Synthetic actin C-terminal peptideSer-Gly-Pro-Ser-Ile-Val-His-Arg-Lys-Cys-Phe,attached to a Multiple Antigen Peptide(MAP) backbone.
Reactivity Chicken, Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Immunohistochemistry(Frozen Section), 4μg/ml, Human, chicken, mouse, rat, -
Western blot, 2μg/ml, Human, chicken, mouse, rat

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F).

Images And Assay Conditions

/antibody/ma1000 1 IHC anti actin antibody monoclonal ac 40.jpg

Anti- Actin antibody, MA1000, IHC(F)
IHC(F): Mouse Intestine Tissue

/antibody/ma1000 2_2 IHC anti actin antibody monoclonal ac 40.jpg

Anti- Actin antibody, MA1000, IHC(F)
IHC(F): Mouse Spleen Tissue

/antibody/ma1000 3 IHC anti actin antibody monoclonal ac 40.jpg

Anti- Actin antibody, MA1000, IHC(F)
IHC(F): Rat Intestine Tissue

/antibody/ma1000 4 IHC anti actin antibody monoclonal ac 40.jpg

Anti- Actin antibody, MA1000, IHC(F)
IHC(F): Rat Spleen Tissue

/antibody/ma1000 5 IHC anti actin antibody monoclonal ac 40.jpg

Anti- Actin antibody, MA1000, IHC(F)
IHC(F): Human Placenta Tissue

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P68133
Gene Name ACTA1
Protein Name Actin, alpha skeletal muscle
Alternative Names Actin, alpha skeletal muscle;Alpha-actin-1;ACTA1;ACTA;
Subcellular Localization Cytoplasm, cytoskeleton.
Molecular Weight 42051 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Background Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in the cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha-actin, one beta-actin, two gamma-actin. Because the amino acid sequence of the C-terminal is the same for almost all actins, this antibody has been raised using a synthetic peptide corresponding to the C-terminal 11 residues.

Order Product (MA1000)

Promotion:

Buy primary get secondary antibody for free.
Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1050 $280
100ug+Free Biotin Secondary BA1001 $280

USD $280

Ships in 5-7 business days.

Troubleshooting

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Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: One other very common name is gamma-actin antibody