Product Info Summary
SKU: | A05553-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Actin-like protein 6A ACTL6A Antibody
SKU/Catalog Number
A05553-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Actin-like protein 6A ACTL6A Antibody catalog # A05553-1. Tested in WB,ICC,IF,IHC,IP applications. This antibody reacts with Human,Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Actin-like protein 6A ACTL6A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05553-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human MSK2 around the phosphorylation site of T568.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05553-1 is reactive to ACTL6A in Human, Mouse
Applications
A05553-1 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
111 kDa
Calculated molecular weight
47.461kDa
Background of BAF53A
Serine/threonine-protein kinase that is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factor RELA, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes. Phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin. Plays an essential role in the control of RELA transcriptional activity in response to TNF. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and EGF, which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 1 (HMGN1/HMG14). In lipopolysaccharide-stimulated primary macrophages, acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines. Functions probably by inducing transcription of the MAP kinase phosphatase DUSP1 and the anti-inflammatory cytokine interleukin 10 (IL10), via CREB1 and ATF1 transcription factors.
Pierrat B.,J. Biol. Chem. 273:29661-29671(1998).
Deak M.,EMBO J. 17:4426-4441(1998).
Soloaga A.,EMBO J. 22:2788-2797(2003).<
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2,000
ICC/IF: 1:50-1:100
IHC: 1:50-1:100
IP: 1:10-1:50
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunocytochemistry staining of ACTL6A using Anti-Actin-like protein 6A ACTL6A Antibody (A05553-1).
ICC staining ACTL6A in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehydeClick image to see more details
Figure 1. Western blotting validation for Anti-Actin-like protein 6A ACTL6A Antibody A05553-1
Western blot analysis of ACTL6A on Jurkat cell(1) and mouse brain tissue(2) lysate using anti-ACTL6A antibody at 1/500 dilution.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For ACTL6A (Source: Uniprot.org, NCBI)
Gene Name
ACTL6A
Full Name
Actin-like protein 6A
Weight
47.461kDa
Superfamily
actin family
Alternative Names
actin-like 6A; actin-like protein 6A; actin-related protein 4,53 kDa BRG1-associated factor A; Actin-related protein Baf53a; Actl6; Arp4; arpNbeta; BAF complex 53 kDa subunit; Baf53a; BAF53AACTL6; BAF53MGC5382; BRG1-associated factor 53A; hArpN beta; INO80 complex subunit K; INO80KARPN-BETA ACTL6A ACTL6, ARPN-BETA, Arp4, BAF53A, INO80K actin like 6A actin-like protein 6A|53 kDa BRG1-associated factor A|BAF complex 53 kDa subunit|BAF53|BRG1-associated factor 53A|INO80 complex subunit K|actin-related protein 4|actin-related protein Baf53a|arpNbeta|hArpN beta
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ACTL6A, check out the ACTL6A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ACTL6A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Actin-like protein 6A ACTL6A Antibody (A05553-1)
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No publications found for A05553-1
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