Anti-ADAM17 Antibody

TACE/ADAM17 antibody

Boster Bio Anti-ADAM17 Antibody catalog # PA1844. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PA1844
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-ADAM17 Antibody

View all TACE/ADAM17 Antibodies

SKU/Catalog Number

PA1844

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ADAM17 Antibody catalog # PA1844. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ADAM17 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1844)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human ADAM17, different from the related rat and mouse sequences by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1844 is reactive to ADAM17 in Human, Mouse, Rat

Applications

PA1844 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

93.021kDa

Background of TACE/ADAM17

ADAM17 (ADAM metallopeptidase domain 17), also called TACE  (tumor necrosis factor-alpha-converting enzyme), is a 70-kDa enzyme that belongs to the ADAM protein family of disintegrins and metalloproteases. Expression studies showed that the encoded protein cleaves precursor tumor necrosis factor-alpha to its mature form. Northern blot analysis revealed that the gene was expressed as a 5-kb mRNA in all tissues examined. ADAM17 is understood to be involved in the processing of tumor necrosis factor alpha (TNF-alpha) at the surface of the cell, and from within theintracellular membranes of the trans-Golgi network. This process, which is also known as "shedding", involves the cleavage and release of a soluble ectodomain from membrane-bound pro-proteins (such as pro-TNF-alpha), and is of known physiological importance. ADAM17 was the first "sheddase" to be identified, and is also understood to play a role in the release of a diverse variety of membrane-anchored cytokines, cell adhesion molecules,receptors, ligands, and enzymes.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For ADAM17 (Source: Uniprot.org, NCBI)

Gene Name

ADAM17

Full Name

Disintegrin and metalloproteinase domain-containing protein 17

Weight

93.021kDa

Alternative Names

ADAM 17; ADAM metallopeptidase domain 17; ADAM metallopeptidase domain 18; ADAM17; ADAM18; CD156b antigen; CD156b; CSVP; disintegrin and metalloproteinase domain-containing protein 17; EC 3.4.24.86; MGC71942; Snake venom-like protease; TACE; TACEcSVP; TNF-alpha convertase; TNF-alpha converting enzyme; TNF-alpha-converting enzyme; tumor necrosis factor, alpha, converting enzyme ADAM17 ADAM18, CD156B, CSVP, NISBD, NISBD1, TACE ADAM metallopeptidase domain 17 disintegrin and metalloproteinase domain-containing protein 17|ADAM metallopeptidase domain 18|TNF-alpha convertase|TNF-alpha convertase enzyme|TNF-alpha converting enzyme|a disintegrin and metalloproteinase 17|cartilage snake venom-like protease|snake venom-like protease|tumor necrosis factor, alpha, converting enzyme

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ADAM17, check out the ADAM17 Infographic

ADAM17 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ADAM17: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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16 Customer Q&As for Anti-ADAM17 Antibody

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for oocyte using anti-ADAM17 antibody PA1844. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-04-06

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-04-06

Question

I have a question about product PA1844, anti-ADAM17 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-12-27

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1844 anti-ADAM17 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-12-27

Question

We have observed staining in mouse cervix carcinoma. Do you have any suggestions? Is anti-ADAM17 antibody supposed to stain cervix carcinoma positively?

Verified Customer

Verified customer

Asked: 2019-11-18

Answer

From literature cervix carcinoma does express ADAM17. From Uniprot.org, ADAM17 is expressed in oocyte, leukocyte monocyte, cartilage, cervix carcinoma, erythroleukemia, liver, among other tissues. Regarding which tissues have ADAM17 expression, here are a few articles citing expression in various tissues:
Cartilage, Pubmed ID: 9574564, 12058067
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Erythroleukemia, Pubmed ID: 23186163
Leukocyte, and Monocyte, Pubmed ID: 9034191
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2019-11-18

Question

Is this PA1844 anti-ADAM17 antibody reactive to the isotypes of ADAM17?

Verified Customer

Verified customer

Asked: 2019-09-23

Answer

The immunogen of PA1844 anti-ADAM17 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human ADAM17(806-824aa AASFKLQRQNRVDSKETEC), different from the related rat and mouse sequences by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-09-23

Question

Is there a BSA free version of anti-ADAM17 antibody PA1844 available?

Verified Customer

Verified customer

Asked: 2019-08-13

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-ADAM17 antibody PA1844 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-08-13

Question

Will anti-ADAM17 antibody PA1844 work on horse WB with erythroleukemia?

Verified Customer

Verified customer

Asked: 2019-07-08

Answer

Our lab technicians have not validated anti-ADAM17 antibody PA1844 on horse. You can run a BLAST between horse and the immunogen sequence of anti-ADAM17 antibody PA1844 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse erythroleukemia in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-07-08

Question

Is a blocking peptide available for product anti-ADAM17 antibody (PA1844)?

Verified Customer

Verified customer

Asked: 2019-05-21

Answer

We do provide the blocking peptide for product anti-ADAM17 antibody (PA1844). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-05-21

Question

My lab would like using your anti-ADAM17 antibody for b cell differentiation studies. Has this antibody been tested with western blotting on panc cell lysate? We would like to see some validation images before ordering.

J. Zhao

Verified customer

Asked: 2018-10-16

Answer

Thanks for your inquiry. This PA1844 anti-ADAM17 antibody is tested on human placenta tissue, tissue lysate, hela cell lysate, panc cell lysate, 293t cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2018-10-16

Question

We are currently using anti-ADAM17 antibody PA1844 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2018-06-21

Answer

The anti-ADAM17 antibody (PA1844) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-06-21

Question

I am looking for to test anti-ADAM17 antibody PA1844 on human oocyte for research purposes, then I may be interested in using anti-ADAM17 antibody PA1844 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

R. Wu

Verified customer

Asked: 2018-06-07

Answer

The products we sell, including anti-ADAM17 antibody PA1844, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-06-07

Question

Would anti-ADAM17 antibody PA1844 work for WB with oocyte?

Verified Customer

Verified customer

Asked: 2018-05-24

Answer

According to the expression profile of oocyte, ADAM17 is highly expressed in oocyte. So, it is likely that anti-ADAM17 antibody PA1844 will work for WB with oocyte.

Boster Scientific Support

Answered: 2018-05-24

Question

I see that the anti-ADAM17 antibody PA1844 works with WB, what is the protocol used to produce the result images on the product page?

B. Mangal

Verified customer

Asked: 2018-01-09

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-01-09

Question

My boss were happy with the WB result of your anti-ADAM17 antibody. However we have been able to see positive staining in liver membrane using this antibody. Is that expected? Could you tell me where is ADAM17 supposed to be expressed?

D. Banerjee

Verified customer

Asked: 2017-04-19

Answer

From what I have seen in literature, liver does express ADAM17. Generally ADAM17 expresses in membrane. Regarding which tissues have ADAM17 expression, here are a few articles citing expression in various tissues:
Cartilage, Pubmed ID: 9574564, 12058067
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Erythroleukemia, Pubmed ID: 23186163
Leukocyte, and Monocyte, Pubmed ID: 9034191
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2017-04-19

Question

Does PA1844 anti-ADAM17 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

L. Moore

Verified customer

Asked: 2015-07-24

Answer

As indicated on the product datasheet, PA1844 anti-ADAM17 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2015-07-24

Question

See below the WB image, lot number and protocol we used for oocyte using anti-ADAM17 antibody PA1844. Please let me know if you require anything else.

W. Li

Verified customer

Asked: 2014-10-09

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-10-09

Question

I was wanting to use your anti-ADAM17 antibody for WB for human oocyte on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human oocyte identification?

J. Krishna

Verified customer

Asked: 2014-09-11

Answer

It shows on the product datasheet, PA1844 anti-ADAM17 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human oocyte in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2014-09-11

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Conjugation

Total: $370

SKU:PA1844

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