|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-ADAM17/Tace Antibody|
|Description||Rabbit IgG polyclonal antibody for Disintegrin and metalloproteinase domain-containing protein 17(ADAM17) detection. Tested with WB in Human;Mouse;Rat.|
|Cite This Product||Anti-ADAM17/Tace Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1844)|
|Replacement Item||This antibody may replace the following items: sc-13973|sc-25782|sc-390859|sc-6416|sc-6417 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human ADAM17(806-824aa AASFKLQRQNRVDSKETEC), different from the related rat and mouse sequences by one amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Disintegrin and metalloproteinase domain-containing protein 17(ADAM 17)|
|Molecular Weight||93021 MW|
|Protein Function||Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Acts as an activator of Notch pathway by mediating cleavage of Notch, generating the membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). Plays a role in the proteolytic processing of ACE2. .|
|Tissue Specificity||Ubiquitously expressed. Expressed at highest levels in adult heart, placenta, skeletal muscle, pancreas, spleen, thymus, prostate, testes, ovary and small intestine, and in fetal brain, lung, liver and kidney.|
|Sequence Similarities||Contains 1 disintegrin domain.|
|Subcellular Localization||Membrane; Single-pass type I membrane protein.|
|Alternative Names||Disintegrin and metalloproteinase domain-containing protein 17;ADAM 17;184.108.40.206;Snake venom-like protease;TNF-alpha convertase;TNF-alpha-converting enzyme;CD156b;ADAM17;CSVP, TACE;|
|Research Areas|||neuroscience|neurology process|neurodegenerative disease|alzheimer's disease| signal transduction|adapters|transmembrane| stem cells|signaling pathways|notch|secreted| cancer|invasion/microenvironment|ecm|extracellular matrix|adam protein family| cell biology|proteolysis / ubiquitin|proteolytic enzymes|metalloprotease|adams|cancer metabolism|response to hypoxia| metabolism|pathways and processes|metabolism processes|types of disease||
Background for Disintegrin and metalloproteinase domain-containing protein 17(ADAM 17)
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-ADAM17/Tace Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-ADAM17/Tace Antibody Images
Click the images to enlarge.
Lane 1: Human Placenta Tissue Lysate
Lane 2: HELA Cell Lysate
Lane 3: PANC Cell Lysate
Lane 4: 293T Cell Lysate
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,