Product Info Summary
SKU: | A00869-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-ADAR Antibody
SKU/Catalog Number
A00869-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-ADAR Antibody catalog # A00869-1. Tested in WB,ICC,IF,IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ADAR Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00869-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human Annexin II around the phosphorylation site of S26.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00869-1 is reactive to ADAR in Human
Applications
A00869-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
136.066kDa
Background of ADAR
Binds double-stranded DNA. Binds preferentially to supercoiled DNA and cruciform DNA structures. Seems to be involved in transcriptional regulation. May function as a transcriptional repressor. Could have a role in the regulation of hematopoietic differentiation through activation of unknown target genes. Controls cellular proliferation by modulating the functions of cell cycle regulatory factors including p53/TP53 and the retinoblastoma protein. May be involved in TP53-mediated transcriptional activation by enhancing TP53 sequence-specific DNA binding and modulating TP53 phosphorylation status. Seems to be involved in energy-level-dependent activation of the ATM/ AMPK/TP53 pathway coupled to regulation of autophagy. May be involved in regulation of TP53-mediated cell death also involving BRCA1. May be involved in the senescence of prostate epithelial cells. Involved in innate immune response by recognizing viral dsDNA in the cytosol and probably in the nucleus. After binding to viral DNA in the cytoplasm recruits TMEM173/STING and mediates the induction of IFN-beta. Has anti-inflammatory activity and inhibits the activation of the AIM2 inflammasome, probably via association with AIM2. Proposed to bind viral DNA in the nucleus, such as of Kaposi's sarcoma-associated herpesvirus, and to induce the formation of nuclear caspase-1-activating inflammasome formation via association with PYCARD. Inhibits replication of herpesviruses such as human cytomegalovirus (HCMV) probably by interfering with promoter recruitment of members of the Sp1 family of transcription factors. Necessary to activate the IRF3 signaling cascade during human herpes simplex virus 1 (HHV-1) infection and promotes the assembly of heterochromatin on herpesviral DNA and inhibition of viral immediate-early gene expression and replication.
Trapani J.A., Immunogenetics 36:369-376(1992).
Trapani J.A., Immunogenetics 40:415-424(1994).
Jiang C., Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2,000
ICC: 1:50-1:200
IHC: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunocytochemistry staining of ADAR using Anti-ADAR Antibody (A00869-1).
ICC staining ADAR in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehydeClick image to see more details
Figure 1. Western blotting validation for Anti-ADAR Antibody A00869-1
Western blot analysis of ADAR on SiHa cell using anti-ADAR antibody at 1/500 dilution.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For ADAR (Source: Uniprot.org, NCBI)
Gene Name
ADAR
Full Name
Double-stranded RNA-specific adenosine deaminase
Weight
136.066kDa
Alternative Names
ADAR1DSH; adenosine deaminase, RNA-specific; DRADAP136; DSRADadenosine deaminase acting on RNA 1-A; EC 3.5.4; EC 3.5.4.-; G1P1double-stranded RNA-specific adenosine deaminase; IFI-4; IFI4dsRNA adenosine deaminase; interferon-induced protein 4; Interferon-inducible protein 4; K88DSRBP; p136,136 kDa double-stranded RNA-binding protein ADAR ADAR1, AGS6, DRADA, DSH, DSRAD, G1P1, IFI-4, IFI4, K88DSRBP, P136 adenosine deaminase RNA specific double-stranded RNA-specific adenosine deaminase|136 kDa double-stranded RNA-binding protein|adenosine deaminase acting on RNA 1-A|dsRNA adenosine deaminase|dsRNA adeonosine deaminase|interferon-induced protein 4|interferon-inducible protein 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ADAR, check out the ADAR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ADAR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ADAR Antibody (A00869-1)
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No publications found for A00869-1
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