Product Info Summary
| SKU: | A00594 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-ADM/Adrenomedullin Antibody
SKU/Catalog Number
A00594
Size
100μl
Form
Liquid
Description
Boster Bio Anti-ADM/Adrenomedullin Antibody catalog # A00594. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ADM/Adrenomedullin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00594)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human ADM. AA range:101-150
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00594 is reactive to ADM in Human, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
20.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00594 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC: 1:100-300
ELISA 1:20000
IF 1:50-200
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemical analysis of paraffin-embedded rat-kidney, antibody was diluted at 1:100
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Western Blot analysis of mouse kidney, mouse lung, mouse heart cells using ADM Polyclonal Antibody. Secondary antibody was diluted at 1:20000
Click image to see more details
Western blot analysis of lysate from mouse kidney cells, using ADM Antibody.
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Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100
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Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100
Specific Publications For Anti-ADM/Adrenomedullin Antibody (A00594)
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Customer Q&As
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7 Customer Q&As for Anti-ADM/Adrenomedullin Antibody
Question
We are currently using anti-ADM/Adrenomedullin antibody A00594 for rat tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, rat. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
The anti-ADM/Adrenomedullin antibody (A00594) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-23
Question
Will anti-ADM/Adrenomedullin antibody A00594 work on bovine WB with kidney?
Verified Customer
Verified customer
Asked: 2019-11-26
Answer
Our lab technicians have not tested anti-ADM/Adrenomedullin antibody A00594 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-ADM/Adrenomedullin antibody A00594 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine kidney in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-26
Question
I was wanting to use to test anti-ADM/Adrenomedullin antibody A00594 on human liver for research purposes, then I may be interested in using anti-ADM/Adrenomedullin antibody A00594 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-02
Answer
The products we sell, including anti-ADM/Adrenomedullin antibody A00594, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-02
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-ADM/Adrenomedullin antibody A00594. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-12-11
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-12-11
Question
Is a blocking peptide available for product anti-ADM/Adrenomedullin antibody (A00594)?
J. Lewis
Verified customer
Asked: 2018-06-18
Answer
We do provide the blocking peptide for product anti-ADM/Adrenomedullin antibody (A00594). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-06-18
Question
I see that the anti-ADM/Adrenomedullin antibody A00594 works with WB, what is the protocol used to produce the result images on the product page?
A. Carter
Verified customer
Asked: 2016-04-04
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2016-04-04
Question
Is there a BSA free version of anti-ADM/Adrenomedullin antibody A00594 available?
F. Zhao
Verified customer
Asked: 2016-02-15
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ADM/Adrenomedullin antibody A00594 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-02-15

