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Product Info Summary
| SKU: | A01757 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-AES Antibody
SKU/Catalog Number
A01757
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-AES Antibody (Catalog # A01757). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
AES antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-AES Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01757)
Host
Rabbit
Contents
AES Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
AES antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human AES. The immunogen is located within the last 50 amino acids of AES.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
TFF3 antibody is predicted to not cross-react with other TFF family members.
Reactive Species
A01757 is reactive to TLE5 in Human, Mouse, Rat
Applications
A01757 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
21.97kDa
Background of TLE5
Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment. Amino-terminal enhancer of split (AES) is a member of the Groucho/ transducin-like enhancer of split (TLE) family of transcriptional regulators, a group of transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1), a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis. It has been suggested that this complex turns off a survival-promoting gene transcription program controlled by the TLE protein family. Interestingly, apoptosis of cells transfected with AES and Bit1 could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
AES antibody can be used for the detection of AES by Western blot at 1 - 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of AES in 293 cell lysate with AES antibody at (A) 1
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Immunocytochemistry of AES in HeLa cells with AES antibody at 10 μg/mL.
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Immunofluorescence of AES in Hela cells with AES antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For TLE5 (Source: Uniprot.org, NCBI)
Gene Name
TLE5
Full Name
TLE family member 5
Weight
21.97kDa
Superfamily
WD repeat Groucho/TLE family
Alternative Names
AES-1; AES-2; Amino enhancer of split; amino-terminal enhancer of split; ESP1; Gp130-associated protein GAM; GRG; GRG5; Protein ESP1; Protein GRG; TLE5 TLE5 AES, AES-1, AES-2, ESP1, GRG, GRG5, Grg-5 TLE family member 5, transcriptional modulator TLE family member 5|amino-terminal enhancer of split|gp130-associated protein GAM|groucho-related protein 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TLE5, check out the TLE5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TLE5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AES Antibody (A01757)
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No publications found for A01757
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