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Product Info Summary
| SKU: | A00520-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-AKT3 Antibody (Center)
SKU/Catalog Number
A00520-1
Size
80 µl
Form
Liquid
Description
Boster Bio Anti-AKT3 Antibody (Center) (Catalog # A00520-1). Tested in WB, IHC-P application(s). This antibody reacts with Human, Mouse, Monkey, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-AKT3 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00520-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This AKT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 88-118 amino acids from the Central region of human AKT3.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00520-1 is reactive to AKT3 in Human, Monkey, Mouse, Rat
Calculated molecular weight
55.8 kDa
Background of AKT3
AKT3 is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00520-1 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:50-1:100
Validation Images & Assay Conditions
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Western blot analysis of lysate from Jurkat cell line,using AKT3 Antibody (Q103).A00520-1 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug per lane.
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Specific Publications For Anti-AKT3 Antibody (Center) (A00520-1)
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