Product Info Summary
SKU: | A00520-1 |
---|---|
Size: | 80 µl |
Reactive Species: | Human, Monkey, Mouse, Rat |
Host: | Rabbit |
Application: | IHC-P, WB |
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Product info
Product Name
Anti-AKT3 Antibody (Center)
SKU/Catalog Number
A00520-1
Size
80 µl
Description
Boster Bio Anti-AKT3 Antibody (Center) (Catalog # A00520-1). Tested in WB, IHC-P application(s). This antibody reacts with Human, Mouse, Monkey, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-AKT3 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00520-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This AKT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 88-118 amino acids from the Central region of human AKT3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00520-1 is reactive to AKT3 in Human, Monkey, Mouse, Rat
Applications
A00520-1 is guaranteed for IHC-P, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
55.775kDa
Background of AKT3
AKT3 is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:50-1:100
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of lysate from Jurkat cell line,using AKT3 Antibody (Q103).A00520-1 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug per lane.
Click image to see more details
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Protein Target Info & Infographic
Gene/Protein Information For AKT3 (Source: Uniprot.org, NCBI)
Gene Name
AKT3
Full Name
RAC-gamma serine/threonine-protein kinase
Weight
55.775kDa
Superfamily
protein kinase superfamily
Alternative Names
Akt3; Akt-3; EC 2.7.11; EC 2.7.11.1; PKB gamma; PKBGDKFZp434N0250; PRKBG; RAC-gamma; v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma) AKT3 MPPH, MPPH2, PKB-GAMMA, PKBG, PRKBG, RAC-PK-gamma, RAC-gamma, STK-2 AKT serine/threonine kinase 3 RAC-gamma serine/threonine-protein kinase|PKB gamma|RAC-gamma serine/threonine protein kinase|v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AKT3, check out the AKT3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AKT3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AKT3 Antibody (Center) (A00520-1)
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No publications found for A00520-1
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