Product Info Summary
SKU: | M00853 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Angiopoietin 1 ANGPT1 Rabbit Monoclonal Antibody
View all Angiopoietin-1 Antibodies
SKU/Catalog Number
M00853
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Angiopoietin 1 ANGPT1 Rabbit Monoclonal Antibody catalog # M00853. Tested in WB application. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Angiopoietin 1 ANGPT1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00853)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
FGC-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Angiopoietin 1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00853 is reactive to ANGPT1 in Human, Rat
Applications
M00853 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
64 kDa
Calculated molecular weight
57.513kDa
Background of Angiopoietin-1
Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Angiopoietin 1 expression in HeLa cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For ANGPT1 (Source: Uniprot.org, NCBI)
Gene Name
ANGPT1
Full Name
Angiopoietin-1
Weight
57.513kDa
Alternative Names
AGP1; AGPT; Ang1; ANG-1; angiopoietin 1; Angiopoietin-1; ANGPT1; KIAA0003 ANGPT1 AGP1, AGPT, ANG1 angiopoietin 1 angiopoietin-1|ANG-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ANGPT1, check out the ANGPT1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ANGPT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Angiopoietin 1 ANGPT1 Rabbit Monoclonal Antibody (M00853)
Hello CJ!
M00853 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Ruan Y,Dong W,Kang L,Lei X,Zhang R,Wang F,Zhu X.The Changes of Twist1 Pathway in Pulmonary Microvascular Permeability in a Newborn Rat Model of Hyperoxia-Induced Acute Lung Injury.Front Pediatr.2020 Apr 23;8:190.doi:10.3389/fped.2020.00190.PMID:32391293;P
Species: Rat
M00853 usage in article: APP:WB, SAMPLE:LUNG TISSUE, DILUTION:1:1000
Hypoxic response elements and Tet-On advanced double-controlled systems regulate?hVEGF165and?angiopoietin-1?gene expression?in vitro
Sun Fl, Wang W, Cheng H, Wang Y, Li L, Xue Jl, Wang Xf, Ai Hx, Zhang L, Xu Jd, Wang Xm, Ji Xm. Plos One. 2014 Jun 30;9(6):E101194. Doi: 10.1371/Journal.Pone.0101194. Ecollection 2014. Morroniside Improves Microvascular Functional Integrity Of The ...
Huang S, Yang N, Liu Y, Gao J, Huang T, Hu L, Zhao J, Li Y, Li C, Zhang X. Int J Mol Med. 2012 Dec;30(6):1410-6. Doi: 10.3892/Ijmm.2012.1147. Epub 2012 Oct 1. Grape Seed Proanthocyanidins Inhibit Colon Cancer-Induced Angiogenesis Throu...
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-Angiopoietin 1 ANGPT1 Rabbit Monoclonal Antibody
Question
Is this M00853 anti-Angiopoietin 1 Rabbit Monoclonal antibody reactive to the isotypes of ANGPT1?
Verified Customer
Verified customer
Asked: 2020-04-01
Answer
The immunogen of M00853 anti-Angiopoietin 1 Rabbit Monoclonal antibody is A synthesized peptide derived from human Angiopoietin 1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-01
Question
Does M00853 anti-Angiopoietin 1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-01-09
Answer
You can see on the product datasheet, M00853 anti-Angiopoietin 1 Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-01-09
Question
Does anti-Angiopoietin 1 Rabbit Monoclonal antibody M00853 work on monkey WB with cauda epididymis?
L. Moore
Verified customer
Asked: 2013-12-23
Answer
Our lab technicians have not validated anti-Angiopoietin 1 Rabbit Monoclonal antibody M00853 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-Angiopoietin 1 Rabbit Monoclonal antibody M00853 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey cauda epididymis in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-12-23