Product Info Summary
SKU: | M03625 |
---|---|
Size: | 100ul |
Reactive Species: | Human |
Host: | Mouse |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-ANP32A Monoclonal Antibody
SKU/Catalog Number
M03625
Size
100ul
Form
Liquid
Description
Boster Bio Anti-ANP32A Monoclonal Antibody catalog # M03625. Tested in ELISA, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ANP32A Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03625)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
8D2
Isotype
IgG
Immunogen
Purified recombinant fragment of human ANP32A expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M03625 is reactive to ANP32A in Human
Applications
M03625 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
28.585kDa
Background of ANP32A
Anti Mesothelin Antibody recognizes Mesothelin that is a glycosyl-phosphatidylinositol–anchored glycoprotein present on the cell surface of various human solid tumors. The mesothelin (MSLN) gene encodes a 71-kDa precursor protein that is processed to a 40-kDa glycosylphosphatidylinositol–anchored protein that composes the mature portion and an NH2 terminal 31-kDa fragment called megakaryocyte-potentiating factor that is released from the cell. Mesothelin is a tumor differentiation antigen present at low levels on a restricted set of normal adult tissues, such as mesothelium, but aberrantly over expressed in mesotheliomas, ovarian, and pancreatic cancers. The biological functions of mesothelin remain elusive. A recent study showed that mesothelin binds to MUC16/CA125, and that this interaction mediates cell adhesion, suggesting that there may be an important role for MUC16/CA125 and mesothelin in the metastatic spread of ovarian cancer.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:200-1:1000
ELISA 1:10000
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunohistochemistry validation of ANP32A using Anti-ANP32A Monoclonal Antibody (M03625).
Immunohistochemistry (IHC) analysis of paraffin-embedded Human Kidney tissue with DAB staining using ANP32A Monoclonal Antibody.For more protocol information of IHC
Click image to see more details
Figure 1. Western blotting validation for Anti-ANP32A Monoclonal Antibody M03625
Western Blot (WB) analysis using ANP32A Monoclonal Antibody against HeLa (1) cell lysate.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For ANP32A (Source: Uniprot.org, NCBI)
Gene Name
ANP32A
Full Name
Acidic leucine-rich nuclear phosphoprotein 32 family member A
Weight
28.585kDa
Superfamily
ANP32 family
Alternative Names
acidic (leucine-rich) nuclear phosphoprotein 32 family, member A; acidic leucine-rich nuclear phosphoprotein 32 family member A; Acidic nuclear phosphoprotein pp32; cerebellar leucine rich acidic nuclear protein; I1PP2A; inhibitor-1 of protein phosphatase-2A; LANPPutative HLA-DR-associated protein I; Leucine-rich acidic nuclear protein; MAPMC15orf1; mapmodulin; PHAP1MGC150373; PHAPIMGC119787; Potent heat-stable protein phosphatase 2A inhibitor I1PP2A; PP32; putative human HLA class II associated protein I ANP32A C15orf1, HPPCn, I1PP2A, LANP, MAPM, PHAP1, PHAPI, PP32 acidic nuclear phosphoprotein 32 family member A acidic leucine-rich nuclear phosphoprotein 32 family member A|acidic (leucine-rich) nuclear phosphoprotein 32 family, member A|acidic nuclear phosphoprotein pp32|cerebellar leucine rich acidic nuclear protein|epididymis secretory sperm binding protein|hepatopoietin Cn|inhibitor-1 of protein phosphatase-2A|leucine-rich acidic nuclear protein|mapmodulin|potent heat-stable protein phosphatase 2A inhibitor I1PP2A|putative HLA-DR-associated protein I|putative human HLA class II associated protein I
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ANP32A, check out the ANP32A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ANP32A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ANP32A Monoclonal Antibody (M03625)
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No publications found for M03625
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Customer Reviews
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