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Product Info Summary
| SKU: | A00333 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IP, IHC, WB |
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Product info
Product Name
Anti-Anti-DDB1 Antibody
SKU/Catalog Number
A00333
Size
100uL
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-Anti-DDB1 Antibody (Catalog # A00333). Tested in IP, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store Anti-DDB1 at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-Anti-DDB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00333)
Host
Rabbit
Contents
0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
Antiserum
Immunogen
DDB1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 198-213 of Human DDB1 (internal) coupled to KLH.
Reactive Species
A00333 is reactive to DDB1 in Human, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
127.0 kDa
Background of DDB1
DDB1 is also known as damage-specific DNA binding protein 1, DDB p127 subunit, DDBa, UV-damaged DNA-binding protein 1, UV-DDB 1, Xeroderma pigmentosum group E complementing protein, XPCe, X-associated protein 1 and XAP-1. The DDB1 gene encodes the large subunit (p127) of DNA damage-binding protein, which is a heterodimer, composed of a large and a small subunit (p48 DDB2). This nuclear protein functions in nucleotide-excision repair resulting from UV-damaged DNA by binding to pyrimidine dimers. Its defective activity causes the repair defect in the patients with xeroderma pigmentosum complementation group E (XPE). XP-E is a rare human autosomal recessive disease characterized by solar sensitivity, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. DDB1 antibody is involved in Epigenetic and Cancer / DNA Damage research.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00333 is guaranteed for ELISA, IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:2,000 - 1:10,000
IHC: User optimized
IP: 1:500
WB: 1:500 - 1:1,000
Anti-DDB1 antibody reacts with human and mouse DDB1 tested by western blot and immunoprecipitation. The antibody immunoprecipitates in vitro translated protein and protein from cell lysates (using HeLa, NIH-3T3, and others). Coimmunoprecipitation of related proteins has not been tested. A 127.0 kDa band corresponding to human DDB1 is detected. Most cell lines expressing DDB1 can be used as a positive control. Researchers should determine optimal titers for other applications.
Validation Images & Assay Conditions
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Most modifiers mature by proteolytic processing from inactive precursors (a; amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S.Jentsch.
Specific Publications For Anti-Anti-DDB1 Antibody (A00333)
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