Anti-Anti-DDB1 Antibody

Product Name

Anti-Anti-DDB1 Antibody

View all DDB1 Antibodies

SKU/Catalog Number

A00333

Size

100uL

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Anti-DDB1 Antibody (Catalog # A00333). Tested in IP, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store Anti-DDB1 at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-Anti-DDB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00333)

Host

Rabbit

Contents

0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Clone Number

2B1

Isotype

Antiserum

Immunogen

DDB1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 198-213 of Human DDB1 (internal) coupled to KLH.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A00333 is reactive to DDB1 in Human, Mouse

Applications

A00333 is guaranteed for IP, WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

126.968kDa

Background of DDB1

DDB1 is also known as damage-specific DNA binding protein 1, DDB p127 subunit, DDBa, UV-damaged DNA-binding protein 1, UV-DDB 1, Xeroderma pigmentosum group E complementing protein, XPCe, X-associated protein 1 and XAP-1. The DDB1 gene encodes the large subunit (p127) of DNA damage-binding protein, which is a heterodimer, composed of a large and a small subunit (p48 DDB2). This nuclear protein functions in nucleotide-excision repair resulting from UV-damaged DNA by binding to pyrimidine dimers. Its defective activity causes the repair defect in the patients with xeroderma pigmentosum complementation group E (XPE). XP-E is a rare human autosomal recessive disease characterized by solar sensitivity, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. DDB1 antibody is involved in Epigenetic and Cancer / DNA Damage research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:2,000 - 1:10,000
IHC: User optimized
IP: 1:500
WB: 1:500 - 1:1,000

Validation Images & Assay Conditions

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Most modifiers mature by proteolytic processing from inactive precursors (a; amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin.Data contributed by S.Jentsch.

Gene/Protein Information For DDB1 (Source: Uniprot.org, NCBI)

Alternative Names

damage-specific DNA binding protein 1 (127kD); damage-specific DNA binding protein 1, 127kDa; Damage-specific DNA-binding protein 1; DDB p127 Subunit; DDB1; DDBA; DNA damage-binding protein 1; DNA damage-binding protein a; HBV X-associated protein 1; UV-damaged DNA-binding factor; UV-damaged DNA-binding protein 1; UV-DDB 1; UV-DDB1; XAP1; XAP-1; Xeroderma pigmentosum group E-complementing protein; XPCE; XPE; XPE-BF; XPE-binding factor DDB1 DDBA, UV-DDB1, XAP1, XPCE, XPE, XPE-BF damage specific DNA binding protein 1 DNA damage-binding protein 1|DDB p127 subunit|DNA damage-binding protein a|HBV X-associated protein 1|UV-DDB 1|UV-damaged DNA-binding factor|UV-damaged DNA-binding protein 1|XAP-1|XPE-binding factor|damage-specific DNA binding protein 1, 127kDa|xeroderma pigmentosum group E-complementing protein

For more info on DDB1, check out the DDB1 Infographic

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In this infographic, you will see the following information for DDB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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